Publications by Hui Yang | EPFL Graph Search

EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization

Determining the spatial organization and morphological characteristics of molecularly defined cell types is a major bottleneck for characterizing the architecture underpinning brain function. We developed Expansion Assisted Iterative Fluorescence In Situ H ...

CELL PRESS2021

Microsphere-based super-resolution scanning optical microscope

Martinus Gijs, Hui Yang, Gergely Huszka

High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...

Optical Soc Amer2017

System for optical detection and imaging of sub-diffraction-limited nano-objects

Martinus Gijs, Hui Yang, Matteo Cornaglia

Nano-objects (8) with feature size smaller than the classical Abbe's diffraction limit can be optically detected via a microlens (4) array configured in a miniaturized microfluidic device. A hydrophilic microwell (3) array in a hydrophobic layer (2) is fir ...

2016

Super-Resolution Imaging of a Dielectric Microsphere Is Governed by the Waist of Its Photonic Nanojet

Martinus Gijs, Hui Yang, Raphaël Etienne Jean Trouillon, Gergely Huszka

Dielectric microspheres with appropriate refractive index can image objects with super-resolution, that is, with a precision well beyond the classical diffraction limit. A microsphere is also known to generate upon illumination a photonic nanojet, which is ...

American Chemical Society2016

Determination of sub-diffraction-limited organelles and biomolecules using microsphere lenses in combination with fluorescence microscopy

Martinus Gijs, Johan Auwerx, Norman Lionel Moullan, Hui Yang

We propose the use of a high-refractive index glass microsphere in combination with a conventional fluorescence microscope for imaging sub-cellular organelles and biomolecules. The microsphere is placed on a sample that is immersed in water, collects the n ...

Spie-Int Soc Optical Engineering2015

Photonic Nanojet Array for Fast Detection of Single Nanoparticles in a Flow

Martinus Gijs, Hui Yang, Matteo Cornaglia

We detect by optical microscopy Au and fluorescent nanopartides (NPs) during their motion in water based medium, using an array of dielectric microspheres that are patterned in a microwell array template. The Microspheres act as lenses focusing the light o ...

American Chemical Society2015

Study of constrained Brownian motion of nanoparticles near an interface using optical tweezers

Thomas Lehnert, Martinus Gijs, Hui Yang, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We demonstrate a method to determine the Brownian motion and the diffusion coefficient of a nanoparticle in water in a plane that is parallel to a solid boundary and as function of the distance normal to that boundary by using an optical tweezers instrumen ...

Spie-Int Soc Optical Engineering2015

Optical microscopy using a glass microsphere for metrology of sub-wavelength nanostructures

Martinus Gijs, Hui Yang

A technique that allows direct optical imaging of nanostructures and determines quantitatively geometric nanofeatures beyond the classical diffraction limit by using high-refractive index glass microspheres is introduced. The glass microsphere is put on a ...

Elsevier2015

Microsphere lenses for microfluidic bio-assays and for super-resolution imaging of biological structures

Hui Yang

EPFL2014

Super-Resolution Biological Microscopy Using Virtual Imaging by a Microsphere Nanoscope

Martinus Gijs, Johan Auwerx, Norman Lionel Moullan, Hui Yang

Wiley-Blackwell2014

Self-assembled melamine microlens arrays for immunofluorescence enhancement

Abdeljalil Sayah, Martinus Gijs, Hüseyin Cumhur Tekin, Hui Yang

Self-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immun ...

2013

Microtextured Substrates and Microparticles Used as in Situ Lenses for On-Chip Immunofluorescence Amplification

Martinus Gijs, Hui Yang

We propose a concept for enhancing the fluorescent detection signal of on-chip immunoassays by using three-dimensional 3 and 8 mu m size microtextured structures as substrates for the assay, which allows exploitation of a large number of fluorophores withi ...

Amer Chemical Soc2013

High-Performance Protein Preconcentrator Using Microchannel-Integrated Nafion Strip

Martinus Gijs, Venkataragavalu Sivagnanam, Meng Shen, Hui Yang

A microfluidic device for protein preconcentration based on the electrokinetic trapping principle is proposed. It comprises a Nafion strip which is simply cut from a commercially available membrane and integrated into a polydimethylsiloxane (PDMS) microflu ...

IEEE2011

Study of Spatio-Temporal Immunofluorescence on Bead Patterns in a Microfluidic Channel

Martinus Gijs, Venkataragavalu Sivagnanam, Hui Yang

We performed a direct immunoassay inside a microfluidic channel on patterned streptavidin-coated beads, which captured fluorescently-labeled biotin target molecules from a continuous flow. We arranged the beads in a dot array at the bottom of the channel a ...

American Institute of Physics2010

On-chip immunoassay using electrostatic assembly of streptavidin-coated bead micropatterns

Martinus Gijs, Caroline Vandevyver, Venkataragavalu Sivagnanam, Bo Song, Hui Yang

We propose an original concept for a sandwich immunoassay that is completely performed on-chip using streptavidin-coated beads as substrate. The latter are electrostatically self-assembled on aminosilane micropatterns at the bottom of a microfluidic channe ...

2009