Publications by Diego Gabriel Dupouy

Efficient AC electrothermal flow (ACET) on-chip for enhanced immunoassays

Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz

Biochemical reaction rates in microfluidic systems are known to be limited by the diffusional transport of reagents, leading often to lowered sensitivity and/or longer detection times in immunoassays. Several methods, including electrically powering electr ...

ROYAL SOC CHEMISTRY2023

Acoustofluidic large-scale mixing for enhanced microfluidic immunostaining for tissue diagnostics

Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz

The usage of microfluidics for automated and fast immunoassays has gained a lot of interest in the last decades. This integration comes with certain challenges, like the reconciliation of laminar flow patterns of micro-scale systems with diffusion-limited ...

ROYAL SOC CHEMISTRY2023

Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections

Martinus Gijs, Diego Gabriel Dupouy, Daniel Migliozzi, Benjamin Pelz

Because of the close interaction between tumors and the immune system, immunotherapies are nowadays considered as the most promising treatment against cancer. In order to define the diagnosis and the subsequent therapy, crucial information about the immune ...

2019

Rapid high-plex staining and simultaneous imaging for immunophenotyping of tissue sections

Martinus Gijs, Diego Gabriel Dupouy, Daniel Migliozzi, Benjamin Pelz

BMC2019

Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study

Martinus Gijs, Diego Gabriel Dupouy, Saska Brajkovic, Fabio Aimi

Breast cancer is a highly heterogeneous disease. The efficacy of tailored therapeutic strategies relies on the precise detection of diagnostic biomarkers by immunohistochemistry (IHC). Therefore, considering the increasing incidence of breast cancer cases, ...

2019

Microfluidic-based automated multiplex immunophenotyping and imaging

Martinus Gijs, Diego Gabriel Dupouy, Giulia Cappi, Daniel Migliozzi, Benjamin Pelz

SPRINGER2018

Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma

Diego Gabriel Dupouy, Saska Brajkovic, Benjamin Pelz

BackgroundAnaplastic lymphoma kinase (ALK) is a key oncogenic driver in lung adenocarcinoma patients and its fusion proteins are routinely assessed. The microfluidic tissue processor (MTP) device is based on a chip-confined low-volume technology allowing f ...

BMC2018

Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections

Martinus Gijs, Diego Gabriel Dupouy, Saska Brajkovic

Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demons ...

Nature Publishing Group2017

Microfluidic network device

Martinus Gijs, Pierre Joris, Ata Tuna Ciftlik, Diego Gabriel Dupouy

Microfluidic network device (2) configured to supply reagents to a biological tissue sampling device (1), comprising a plurality of microfluidic inlet channels (12) connected to respective sources of said reagents, at least one common outlet channel (22), ...

2017

Method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof

Martinus Gijs, Déborah Heintze, Ata Tuna Ciftlik, Diego Gabriel Dupouy

The invention relates to a method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof. In particular, the invention relates to a method which is a useful tool for use in the field of diagnosis, prevention and/or treat ...

2017

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Martinus Gijs, Déborah Heintze, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Maryse Fiche

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Nature Research2016

Microfluidic staining technology and automated image processing for fast and accurate tissue-based diagnostics

Diego Gabriel Dupouy

The development of microfluidics has led to significant improvements in many areas of in vitro diagnostics. This thesis proposes the use of microfluidic device technology in the field of anatomic pathology, leading to faster and more accurate tissue-based ...

EPFL2016

Programming and use of Parylene C fluorescence as a quantitative on-chip reference

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Joan Teixidor

A large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parame ...

Royal Soc Chemistry2014

Programmable parylene-C bonding layer fluorescence for storing information on microfluidic chips

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy

We demonstrate data storage on glass/silicon microfluidic devices fabricated using parylene-C as a bonding layer. In particular, we report intermediate parylene-C bonding layer fluorescence (iPBLF) and its use as an on-chip medium for data storage by dynam ...

Royal Society of Chemistry2013

Use of a Parylene-C bonding layer fluorescence as reference for on-chip imaging and detection applications

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy

2013