Publications by Romain Hamelin

Differential role of C-terminal truncations on alpha-synuclein pathology and Lewy body formation

Graham Knott, Mary-Claude Croisier-Coeytaux, Anass Chiki, Nadine Aït Bouziad, Romain Hamelin, Anne-Laure Mahul Mellier, Niran Maharjan, Melek Firat Altay, Jonathan Jean-Pierre Ricci, Somanath Jagannath, Galina Limorenko, Salvatore Novello, Yllza Jasiqi

Alpha-synuclein (aSyn) post-translational modifications (PTM), especially phosphorylation at serine 129 and C-terminal truncations, are highly enriched in Lewy bodies (LB), Lewy neurites, and other pathological aggregates in Parkinson's disease and synucle ...

Springer Science and Business Media LLC2025

Casein Kinases 2-dependent phosphorylation of the placental ligand VAR2CSA regulates Plasmodium falciparum-infected erythrocytes cytoadhesion

Romain Hamelin

Placental malaria is characterized by the massive accumulation and sequestration of infected erythrocytes in the placental intervillous blood spaces, causing severe birth outcomes. The variant surface antigen VAR2CSA is associated with Plasmodium falciparu ...

2025

Conditional essentiality of the 11-subunit complex I-like enzyme in strict anaerobes: the case of Desulfitobacterium hafniense strain DCB-2

Christof Holliger, Julien Maillard, Florence Armand, Romain Hamelin, Mathilde Stéphanie Willemin

In oxidative phosphorylation, respiratory complex I serves as an entry point in the electron transport chain for electrons generated in catabolic processes in the form of NADH. An ancestral version of the complex, lacking the NADH-oxidising module, is enco ...

Frontiers Media SA2024

Organ-specific electrophile responsivity mapping in live C. elegans

Romain Hamelin, Balázs Álmos Novotny, Daniel Ahmet Urul, Amogh Kulkarni

Proximity labeling technologies are limited to indexing localized protein residents. Such data—although valuable—cannot inform on small-molecule responsivity of local residents. We here bridge this gap by demonstrating in live C. elegans how electrophile-s ...

2024

Phosphoproteomics and morphology of stored human red blood cells treated by protein tyrosine phosphatases inhibitor

Gerardo Turcatti, Benjamin Rappaz, Florence Armand, Romain Hamelin

The process of protein phosphorylation is involved in numerous cell functions. In particular, phosphotyrosine (pY) has been reported to play a role in red blood cell (RBC) functions, including the cytoskeleton organization. During their storage before tran ...

2023

Resistance mechanism to Notch inhibition and combination therapy in human T-cell acute lymphoblastic leukemia

Freddy Radtke, Nadine Fournier, Florence Armand, Romain Hamelin, Linlin Cao, Maria Pavlou

Gain-of-function mutations in NOTCH1 are among the most frequent genetic alterations in T-cell acute lymphoblastic leukemia (T-ALL), highlighting the Notch signaling pathway as a promising therapeutic target for personalized medicine. Yet, a major limitati ...

2023

Proteome adaptations of the organohalide-respiring Desulfitobacterium hafniense strain DCB-2 to various energy metabolisms

Christof Holliger, Julien Maillard, Florence Armand, Romain Hamelin, Mathilde Stéphanie Willemin

IntroductionDesulfitobacterium hafniense was isolated for its ability to use organohalogens as terminal electron acceptors via organohalide respiration (OHR). In contrast to obligate OHR bacteria, Desulfitobacterium spp. show a highly versatile energy meta ...

Frontiers Media SA2023

Regulation of Src tumor activity by its N-terminal intrinsically disordered region

Florence Armand, Romain Hamelin

The membrane-anchored Src tyrosine kinase is involved in numerous pathways and its deregulation is involved in human cancer. Our knowledge on Src regulation relies on crystallography, which revealed intramolecular interactions to control active Src conform ...

SPRINGERNATURE2022

Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical locales

Romain Hamelin, Yimon Aye, Yi Zhao, Pierre Alexander Miranda Herrera

Enzyme-assisted posttranslational modifications (PTMs) constitute a major means of signaling across different cellular compartments. However, how nonenzymatic PTMs-despite their direct relevance to covalent drug development-impinge on crosscompartment sign ...

National Academy of Sciences2022

Small intestinal resident eosinophils maintain gut homeostasis following microbial colonization

Florence Armand, Nicola Harris, Romain Hamelin, Kathleen Shah, Mati André Marie Moyat

The intestine harbors a large population of resident eosinophils, yet the function of intestinal eosinophils has not been explored. Flow cytometry and whole-mount imaging identified eosinophils residing in the lamina propria along the length of the intesti ...

CELL PRESS2022

Hypoxic Incubation Conditions for Optimized Manufacture of Tenocyte-Based Active Pharmaceutical Ingredients of Homologous Standardized Transplant Products in Tendon Regenerative Medicine

Diego Chiappe, Florence Armand, Romain Hamelin

Human fetal progenitor tenocytes (hFPT) produced in defined cell bank systems have recently been characterized and qualified as potential therapeutic cell sources in tendon regenerative medicine. In view of further developing the manufacture processes of s ...

MDPI2021

Analysis of the S. pombe Meiotic Proteome Reveals a Switch from Anabolic to Catabolic Processes and Extensive Post-transcriptional Regulation

Marc Moniatte, Diego Chiappe, Viesturs Simanis, Andrea Krapp, Elena Cano, Florence Armand, Romain Hamelin, Lucien Fabrice Krapp

Meiotic progression in S. pombe is regulated by stage-specific gene expression and translation, changes in RNA stability, expression of anti-sense transcripts, and targeted proteolysis of regulatory proteins. We have used SILAC labeling to examine the rela ...

2019

Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients

Melody Swartz, Davide Demurtas, Lambert Charles François Potin, Patricia Corthésy Henrioud, Léa Maillat, Romain Hamelin, Natacha Anne-Sophie Bordry, Maria Anna Sofia Broggi, Alexandre Harari

Liquid biopsies allow monitoring of cancer progression and detection of relapse, but reliable biomarkers in melanoma are lacking. Because secreted factors preferentially drain to lymphatic vessels before dilution in the blood, we hypothesized that lymph sh ...

ROCKEFELLER UNIV PRESS2019

Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles

Tim Beltraminelli, Romain Hamelin, Sina Nassiri, Michele De Palma, Chiara Cianciaruso, Bruno Torchia

Extracellular vesicles (EVs), including exosomes, modulate multiple aspects of cancer biology. Tumor-associated macrophages (TAMs) secrete EVs, but their molecular features and functions are poorly characterized. Here, we report methodology for the enrichm ...

2019

Phosphorylation of the VAR2CSA extracellular region is associated with enhanced adhesive properties to the placental receptor CSA

Marc Moniatte, Florence Armand, Romain Hamelin

Plasmodium falciparum is the main cause of disease and death from malaria. P. falciparum virulence resides in the ability of infected erythrocytes (IEs) to sequester in various tissues through the interaction between members of the polymorphic P. falciparu ...

2019

Phosphorylation Of The Var2Csa Extracellular Region Is Associated With Enhanced Adhesive Properties To The Placental Receptor Csa

Marc Moniatte, Florence Armand, Romain Hamelin, Zeineb Fourati

AMER SOC TROP MED & HYGIENE2019

Lysozyme-Induced Transcriptional Regulation of TNF-alpha Pathway Genes in Cells of the Monocyte Lineage

Paul Joseph Dyson, Florence Armand, Romain Hamelin, Gianni Sava, Isabelle Mireille Claude Schepens

Lysozyme is one of the most important anti-bacterial effectors in the innate immune system of animals. Besides its direct antibacterial enzymatic activity, lysozyme displays other biological properties, pointing toward a significant anti-inflammatory effec ...

2019

The membrane-bound C subunit of reductive dehalogenases: topology analysis and reconstitution of the FMN-binding domain of PceC

Julien Maillard, Aamani Rupakula Boyanapalli, Romain Hamelin, Géraldine Florence Buttet, Mathilde Stéphanie Willemin

Organohalide respiration (OHR) is the energy metabolism of anaerobic bacteria able to use halogenated organic compounds as terminal electron acceptors. While the terminal enzymes in OHR, so-called reductive dehalogenases, are well-characterized, the identi ...

Frontiers Media SA2018

The Membrane-Bound C Subunit of Reductive Dehalogenases: Topology Analysis and Reconstitution of the FMN-Binding Domain of PceC

Julien Maillard, Aamani Rupakula Boyanapalli, Romain Hamelin, Géraldine Florence Buttet, Mathilde Stéphanie Willemin

Organohalide respiration (OHR) is the energy metabolism of anaerobic bacteria able to use halogenated organic compounds as terminal electron acceptors. While the terminal enzymes in OHR, so-called reductive dehalogenases, are well-characterized, the identi ...

Frontiers Media SA2018

Differential signaling networks of Bcr-Abl p210 and p190 kinases in leukemia cells defined by functional proteomics

Romain Hamelin, Oliver Hantschel, Sandrine Madeleine Suzanne Georgeon, Sina Maren Reckel

The two major isoforms of the oncogenic Bcr-Abl tyrosine kinase, p210 and p190, are expressed upon the Philadelphia chromosome translocation. p210 is the hallmark of chronic myelogenous leukemia, whereas p190 occurs in the majority of B-cell acute lymphobl ...

Nature Publishing Group2017