Publications by Anton Kozhinov

Adding Color to Mass Spectra of Biopolymers: Charge Determination Analysis (CHARDA) Assigns Charge State to Every Ion Peak

Yury Tsybin, Laure Menin, Anton Kozhinov, Natalia Gasilova, Konstantin Nagornov

Traditionally, mass spectrometry (MS) output is the ion abundance plotted versus the ionic mass-to-charge ratio m/z. While employing only commercially available equipment, Charge Determination Analysis (CHARDA) adds a third dimension to MS, estimating for ...

Amer Chemical Soc2024

Spatially-distributed cyclotron oscillators approach to FT-ICR MS at the true cyclotron frequency: Computational evaluation of sensitivity

Yury Tsybin, Anton Kozhinov, Konstantin Nagornov

Routine Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) with ion detection at the unreduced (true) cyclotron frequency, instead of the reduced cyclotron frequency, has recently been demonstrated experimentally and supported computat ...

ELSEVIER2021

Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody-Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry

Yury Tsybin, Laure Menin, Anton Kozhinov, Natalia Gasilova, Konstantin Nagornov

The therapeutic efficacy and pharmacokinetics of antibody-drug conjugates (ADCs) in general, and antibody-oligonucleotide conjugates (AOCs) in particular, depend on the drug-to-antibody ratio (DAR) distribution and average value. The DAR is considered a cr ...

AMER CHEMICAL SOC2021

Multiplexed Middle-Down Mass Spectrometry as a Method for Revealing Light and Heavy Chain Connectivity in a Monoclonal Antibody

Yury Tsybin, Laure Menin, Luca Fornelli, Anton Kozhinov, Natalia Gasilova, Kristina Srzentic, Konstantin Nagornov, Daniel Ayoub

Pairing light and heavy chains in monoclonal antibodies (mAbs) using top-down (TD) or middle-down (MD) mass spectrometry (MS) may complement the sequence information on single chains provided by high-throughput genomic sequencing and bottom-up proteomics, ...

AMER CHEMICAL SOC2018

Device and Method for Ion Cyclotron Resonance Mass Spectrometry

Yury Tsybin, Anton Kozhinov, Konstantin Nagornov

The present invention relates to a method and device for measuring m/z ratios of ions in ion cyclotron resonance (ICR) mass spectrometry. The described ion traps for ICR mass spectrometry are distinct from the previous configurations by having one or many ...

2017

Ion Trap with Narrow Aperture Detection Electrodes for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Yury Tsybin, Anton Kozhinov, Konstantin Nagornov

The current paradigm in ion trap (cell) design for Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) is the ion detection with wide aperture detection electrodes. Specifically, excitation and detection electrodes are typically 90A deg ...

Springer2015

Producing absorption mode Fourier transform ion cyclotron resonance mass spectra with non-quadratic phase correction functions

Yury Tsybin, Konstantin Zhurov, Anton Kozhinov, Konstantin Nagornov

RATIONALE: Previously described methods for producing absorption mode Fourier transform ion cyclotron resonance (FTICR) mass spectra have all relied on the phase correction function being quadratic. This assumption has been found to be invalid for some ins ...

Wiley-Blackwell2015

Fourier transform mass spectrometry at the uncertainty principle limit for improved qualitative and quantitative molecular analyses

Anton Kozhinov

Nowadays, among the instrumentation park of mass spectrometry, Fourier transform mass spectrometers (FTMS), including ion cyclotron resonance (ICR) and Orbitrap FTMS, provide the highest analytical performance for accurate measurements and mass resolution. ...

EPFL2015

Distinguishing analyte from noise components in mass spectra of complex samples: where to cut the noise?

Yury Tsybin, Konstantin Zhurov, Luca Fornelli, Anton Kozhinov

Fourier transform mass spectrometry (FTMS) enables comprehensive analysis of complex molecular mixtures. Given the broad intensity ranges of components in the mass spectra, it is imperative to accurately determine a noise threshold level above which peak a ...

Amer Chemical Soc2014

High-Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry with Increased Throughput for Biomolecular Analysis

Yury Tsybin, Anton Kozhinov, Konstantin Nagornov

A multielectrode ion cyclotron resonance (ICR) cell, herein referred to as the 4X cell, for signal detection at the quadruple frequency multiple was implemented and characterized on a commercial 10 T Fourier transform ICR mass spectrometer (FT-ICR MS). Not ...

Amer Chemical Soc2014

Least-Squares Fitting of Time-Domain Signals for Fourier Transform Mass Spectrometry

Tagir Aushev, Yury Tsybin, Anton Kozhinov

To advance Fourier transform mass spectrometry (FTMS)-based molecular structure analysis, corresponding development of the FTMS signal processing methods and instrumentation is required. Here, we demonstrate utility of a least-squares fitting (LSF) method ...

Springer2014

Evaluation of high-field Orbitrap Fourier transform mass spectrometer for petroleomics

Yury Tsybin, Konstantin Zhurov, Anton Kozhinov

The performance of the high-field Orbitrap Fourier transform mass spectrometer (FTMS) is evaluated in the context of petroleum sample analysis. Pertinent characteristics including resolving power, mass accuracy, and spectral dynamic range are tested with r ...

Amer Chemical Soc2013

Practical Considerations for Improving the Productivity of Mass Spectrometry-based Proteomics

Yury Tsybin, Luca Fornelli, Anton Kozhinov, Oxana Upir, Unige Anna Laskay, Kristina Srzentic

Mass spectrometry (MS) is currently the most sensitive and selective analytical technique for routine peptide and protein structure analysis. Top-down proteomics is based on tandem mass spectrometry (MS/MS) of intact proteins, where multiply charged precur ...

Schweizerische Chemische Gesellschaft2013

Iterative Method for Mass Spectra Recalibration via Empirical Estimation of the Mass Calibration Function for Fourier Transform Mass Spectrometry-Based Petroleomics

Yury Tsybin, Konstantin Zhurov, Anton Kozhinov

We describe a mass spectra recalibration method, which enables analysis of petroleum samples with Orbitrap FTMS. In this method, the mass calibration function is estimated on the basis of mass-to-charge ratios and abundances of internal calibrants without ...

Amer Chemical Soc2013

Hexagonal Class Representation for Fingerprinting and Facile Comparison of Petroleomic Samples

Yury Tsybin, Konstantin Zhurov, Anton Kozhinov

Because of the high complexity of petroleomic-type samples, there is a need for efficient ways of visualizing and interpreting the resulting data in mass spectrometry-based petroleomics. Over the years, several graphing approaches have become widespread, y ...

Amer Chemical Soc2013

Sidebands in Fourier transform ion cyclotron resonance mass spectra

Yury Tsybin, Anton Kozhinov, Sasa Miladinovic

Sidebands in mass spectra are an intrinsic feature of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Appearance of the sidebands there is detrimental for the analytical performance, especially in case of complex mixtures analyzed ...

Elsevier2012

On the Utility of Isotopic Fine Structure Mass Spectrometry in Protein Identification

Yury Tsybin, Anton Kozhinov, Sasa Miladinovic

Modern mass spectrometry (MS)-based protein identification and characterization relies upon accurate mass measurements of the C-13 isotopic distributions of the enzymatically produced peptides. Interestingly, obtaining peptide elemental composition informa ...

2012

Filter Diagonalization Method-Based Mass Spectrometry for Molecular and Macromolecular Structure Analysis

Yury Tsybin, Anton Kozhinov

Molecular and macromolecular structure analysis by high resolution and accurate mass spectrometry (MS) is indispensable for a number of fundamental and applied research areas, including health and energy domains. Comprehensive structure analysis of molecul ...

2012

High-Resolution and Tandem Mass Spectrometry – the Indispensable Tools of the XXI Century

Yury Tsybin, Aleksey Vorobyev, Luca Fornelli, Anton Kozhinov, Sasa Miladinovic

Mass spectrometry-based qualitative and quantitative (bio)molecular analysis is a corner stone in the state-of-the-art pipelines in systems biology and environmental sciences. High-resolution and efficient tandem mass spectrometry methods and techniques ar ...

Schweizerische Chemische Gesellschaft2011