Publications by Andrea Capozzi

Long-lived enhanced magnetization-A practical metabolic MRI contrast material

Noninvasive tracking of biochemical processes in the body is paramount in diagnostic medicine. Among the leading techniques is spectroscopic magnetic resonance imaging (MRI), which tracks metabolites with an amplified (hyperpolarized) magnetization signal ...

2024

129Xe Dynamic Nuclear Polarization Demystified: The Influence of the Glassing Matrix on the Radical Properties

Rolf Gruetter, Jean-Noël Hyacinthe, Andrea Capozzi, Thanh Phong Kevin Lê, Emma Linnea Wiström

129Xe dissolution dynamic nuclear polarization (DNP) is a controversial topic. The gold standard technique for hyperpolarized xenon magnetic resonance imaging (MRI) is spin exchange optical pumping, which received FDA approval in 2022. Nevertheless, the ve ...

2024

Multi-sample/multi-nucleus parallel polarization and monitoring enabled by a fluid path technology compatible cryogenic probe for dissolution dynamic nuclear polarization

Jean-Noël Hyacinthe, Andrea Capozzi, Thanh Phong Kevin Lê

Low throughput is one of dissolution Dynamic Nuclear Polarization (dDNP) main shortcomings. Especially for clinical and preclinical applications, where direct 13C nuclei polarization is usually pursued, it takes hours to generate one single hyperpolarized ...

Nature Research2023

How to improve the efficiency of a traditional dissolution dynamic nuclear polarization (dDNP) apparatus: Design and performance of a fluid path compatible dDNP/LOD-ESR probe

Jean-Noël Hyacinthe, Andrea Capozzi, Thanh Phong Kevin Lê

Dissolution Dynamic Nuclear Polarization (dDNP) was invented almost twenty years ago. Ever since, hardware advancement has observed 2 trends: the quest for DNP at higher field and, more recently, the development of cryogen free polarizers. Despite the DNP ...

Academic Press Inc - Elsevier Science2022

Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples

Andrea Capozzi

As of today, dissolution Dynamic Nuclear Polarization (dDNP) is the only clinically available hyperpolarization technique for C-13-MRI. Despite the clear path towards personalized medicine that dDNP is paving as an alternative and/or complement to Positron ...

Nature Portfolio2022

Radical-free hyperpolarized MRI using endogenously occurring pyruvate analogues and UV-induced nonpersistent radicals

Rolf Gruetter, Josefina Adriana Maria Bastiaansen, Lionel Pierre Arn, Andrea Capozzi, Hikari Ananda Infinity Yoshihara, Claudia Christina Zanella, Alice Radaelli

It was recently demonstrated that nonpersistent radicals can be generated in frozen solutions of metabolites such as pyruvate by irradiation with UV light, enabling radical-free dissolution dynamic nuclear polarization. Although pyruvate is endogenous, the ...

WILEY2021

Direct measurement of the triple spin flip rate in dynamic nuclear polarization

Andrea Capozzi

A previous study of the effect of Gadolinium doping on the dynamic polarization (DNP) of C-13 using trityls showed that the rate at which the polarization builds up is almost independent of the Gadolinium concentration, while the electron spin-lattice rela ...

Academic Press Inc - Elsevier Science2021

Measuring Glycolytic Activity with Hyperpolarized [2H7, U-13C6] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions

Yves Pilloud, Bernard Lanz, Rolf Gruetter, Mor-Miri Mishkovsky, Emmanuelle Ines Flatt, Andrea Capozzi

Glucose is the primary fuel for the brain; its metabolism is linked with cerebral function. Different magnetic resonance spectroscopy (MRS) techniques are available to assess glucose metabolism, providing complementary information. Our first aim was to inv ...

2021

Hyperpolarization via dissolution dynamic nuclear polarization: new technological and methodological advances

Andrea Capozzi, Arthur César Pinon

Dissolution-DNP is a method to boost liquid-state NMR sensitivity by several orders of magnitude. The technique consists in hyperpolarizing samples by solid-state dynamic nuclear polarization at low temperature and moderate magnetic field, followed by an i ...

SPRINGER2021

Metabolic contrast agents produced from transported solid C-13-glucose hyperpolarized via dynamic nuclear polarization

Andrea Capozzi, Arthur César Pinon

Magnetic Resonance Imaging combined with hyperpolarized C-13-labelled metabolic contrast agents produced via dissolution Dynamic Nuclear Polarization can, non-invasively and in real-time, report on tissue specific aberrant metabolism. However, hyperpolariz ...

NATURE RESEARCH2021

C-13 Dynamic Nuclear Polarization using SA-BDPA at 6.7 T and 1.1 K: Coexistence of Pure Thermal Mixing and Well-Resolved Solid Effect

Rolf Gruetter, Andrea Capozzi, Hikari Ananda Infinity Yoshihara, Alice Radaelli

SA-BDPA is a water-soluble, narrow-line width radical previously used for dynamic nuclear polarization (DNP) signal enhancement in solid-state magic angle spinning NMR spectroscopy. Here, we report the first study using SA-BDPA under dissolution DNP condit ...

AMER CHEMICAL SOC2020

UV-Irradiated 2-Keto-(1-C-13)Isocaproic Acid for High-Performance C-13 Hyperpolarized MR

Andrea Capozzi, Arthur César Pinon

Enhancing the sensitivity of magnetic resonance spectroscopy/imaging (MRS/MRI) by dissolution dynamic nuclear polarization (dDNP) has expanded the scope of MRS applications to new fields of research. Most importantly, it has paved the way toward noninvasiv ...

AMER CHEMICAL SOC2020

Probing cardiac metabolism by hyperpolarized 13C MR using an exclusively endogenous substrate mixture and photo-induced nonpersistent radicals

Arnaud Comment, Rolf Gruetter, Josefina Adriana Maria Bastiaansen, Andrea Capozzi, Hikari Ananda Infinity Yoshihara

Purpose To probe the cardiac metabolism of carbohydrates and short chain fatty acids simultaneously in vivo following the injection of a hyperpolarized 13C-labeled substrate mixture prepared using photo-induced nonpersistent radicals. Methods Droplets of m ...

Wiley2018

Thermal annihilation of photo-induced radicals following dynamic nuclear polarization to produce transportable frozen hyperpolarized 13C-substrates

Arnaud Comment, Giovanni Boero, Christophe Roussel, Tian Cheng, Andrea Capozzi

Hyperpolarization via dynamic nuclear polarization (DNP) is pivotal for boosting magnetic resonance imaging (MRI) sensitivity and dissolution DNP can be used to perform in vivo real-time 13C MRI. The type of applications is however limited by the relativel ...

Nature Publishing Group2017

Methods to hyperpolarize nuclear spins via dissolution and sublimation DNP at high magnetic field

Andrea Capozzi

Dynamic Nuclear Polarization (DNP) is, nowadays, the most well-established and widespread Nuclear Magnetic Resonance (NMR) hyperpolarization technique. With respect to other methods, the main advantage of DNP is, with no doubt, its versatility. In theory e ...

EPFL2016

Optimal Glass-Forming Solvent Brings Sublimation Dynamic Nuclear Polarization to 129Xe Hyperpolarization Biomedical Imaging Standards

Arnaud Comment, Christophe Roussel, Jean-Noël Hyacinthe, Andrea Capozzi

In addition to the dedicated spin exchange optical pumping (SEOP) technique, an alternative method to produce hyperpolarized xenon gas using dynamic nuclear polarization (DNP) followed by a sublimation procedure was proposed. The challenge associated with ...

American Chemical Society2015

Photoinduced Nonpersistent Radicals as Polarizing Agents for X-Nuclei Dissolution Dynamic Nuclear Polarization

Arnaud Comment, Jacques Van Der Klink, Giovanni Boero, Christophe Roussel, Tian Cheng, Tim Rolf Eichhorn, Jean-Noël Hyacinthe, Andrea Capozzi

Hyperpolarization via dissolution dynamic nuclear polarization (DNP) is a versatile method to dramatically enhance the liquid-state NMR signal of X-nuclei and can be used for performing metabolic and molecular imaging. It was recently demonstrated that ins ...

American Chemical Society2015

Radical-free mixture of co-polarized 13C metabolites for probing separate biochemical pathways in vivo by hyperpolarized 13C MR

Arnaud Comment, Rolf Gruetter, Josefina Adriana Maria Bastiaansen, Andrea Capozzi, Hikari Ananda Infinity Yoshihara

2015

In vivo real-time metabolic imaging using frequency selective bSSFP and hyperpolarized compounds

Arnaud Comment, Jean-Noël Hyacinthe, Najat Salameh, Andrea Capozzi

2014

Hyperpolarization of 129Xe by sublimation DNP using photo-activated radical precursors

Arnaud Comment, Christophe Roussel, François Lazeyras, Tim Rolf Eichhorn, Jean-Noël Hyacinthe, Najat Salameh, Andrea Capozzi

2013