Publications by Saba Rezakhani

Bioengineered Tubular Biliary Organoids

Matthias Lütolf, Saba Rezakhani, Mikhail Nikolaev, Bilge Sen Elçi

Liver organoids have emerged as promising in vitro models for toxicology, drug discovery, and disease modeling. However, conventional 3D epithelial organoid culture systems suffer from significant drawbacks, including limited culture duration, a nonphysiol ...

Wiley2024

Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice

Matthias Lütolf, Saba Rezakhani, Antonius Chrisnandy

Glandular epithelia, including mammary gland (MG) and prostate, are composed of luminal and basal cells. During embryonic development, glandular epithelia arise from multipotent stem cells (SCs) that are replaced after birth by unipotent basal and unipoten ...

Nature Research2024

Embryo-uterine interaction coordinates mouse embryogenesis during implantation

Matthias Lütolf, Saba Rezakhani, Mikhail Nikolaev

Embryo implantation into the uterus marks a key transition in mammalian development. In mice, implantation is mediated by the trophoblast and is accompanied by a morphological transition from the blastocyst to the egg cylinder. However, the roles of tropho ...

WILEY2023

Deterministic scRNA-seq captures variation in intestinal crypt and organoid composition

Matthias Lütolf, Bart Deplancke, Saba Rezakhani, Esther Amstad, Riccardo Dainese, Julie Marie Russeil, Antonius Chrisnandy, Vincent Roland Julien Gardeux, Johannes Julius Bues, Marjan Biocanin, Rita Sarkis, Jörn Pezoldt

DisCo is a deterministic droplet microfluidics tool for single-cell analysis on low cell input samples, which is demonstrated to profile individual intestinal organoids and in vivo-derived small tissues. ...

NATURE PORTFOLIO2022

Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis

Matthias Lütolf, Delphine Lorraine Perle Blondel, Saba Rezakhani, Antonius Chrisnandy, Nicolas Antoine Vincent Broguiere

Epithelial organoids are most efficiently grown from mouse-tumour-derived, reconstituted extracellular matrix hydrogels, whose poorly defined composition, batch-to-batch variability and immunogenicity limit clinical applications. Efforts to replace such il ...

NATURE PORTFOLIO2022

A functional genetic toolbox for human tissue-derived organoids

Matthias Lütolf, Saba Rezakhani

Human organoid systems recapitulate key features of organs offering platforms for modelling developmental biology and disease. Tissue-derived organoids have been widely used to study the impact of extrinsic niche factors on stem cells. However, they are ra ...

eLIFE SCIENCES PUBL LTD2021

Defined matrices for epithelial organoid culture and disease modelling

Saba Rezakhani

Advances in our understanding of the biology of stem cells and their niches have provided the necessary foundation to create in vitro complex self-organizing three-dimensional (3D) structures called organoids. While organoids represent a highly promising n ...

EPFL2021

Mechano-modulatory synthetic niches for liver organoid derivation

Matthias Lütolf, Kristina Schoonjans, Saba Rezakhani, Ece Yildiz, Giovanni Sorrentino

The recent demonstration that primary cells from the liver can be expanded in vitro as organoids holds enormous promise for regenerative medicine and disease modelling. The use of three-dimensional (3D) cultures based on ill-defined and potentially immunog ...

Nature Publishing Group2020

Low-Defect Thiol-Michael Addition Hydrogels as Matrigel Substitutes for Epithelial Organoid Derivation

Matthias Lütolf, Saba Rezakhani, Nikolche Gjorevski

Due to their selective crosslinking chemistry and well-defined mechano-biochemical characteristics, hydrogels crosslinked by thiol-Michael addition reactions have garnered immense interest for 3D cell culture applications. These hydrogels are typically for ...

2020

Three dimensional models of tissue fibrosis

Matthias Lütolf, Kristina Schoonjans, Saba Rezakhani, Ece Yildiz, Giovanni Sorrentino

The invention provides a method for obtaining a three-dimensional (3D) cellular model of tissue fibrosis, such as liver fibrosis, comprising culturing epithelial cells in a 3D hydrogel in conditions suitable for a three-dimensional cellular cluster formati ...

2020

Design and Synthesis of Gatekeeper Coated Dendritic Silica/Titania Mesoporous Nanoparticles with Sustained and Controlled Drug Release Properties for Targeted Synergetic Chemo-Sonodynamic Therapy

Saba Rezakhani, Samira Malekmohammadi

New dendritic silica/titania mesoporous nanoparticles (DSTNs) loaded with curcumin (CUR) were synthesized and coated with polyethylenimine-folic acid groups (PEI-FA) for an ultrasound (US)-triggered drug release and combined chemo-sonodynamic therapy. The ...

2019