Publications by Vitalijs Zubkovs

Micropreparative Gel Electrophoresis for Purification of Nanoscale Bioconjugates

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Sayyed Hashem Sajjadi, Shang-Jung Wu, Yahya Rabbani

Conventional techniques for purifying macromolecular conjugates often require complex and costly installments that are inaccessible to most laboratories. In this work, we develop a one-step micropreparative method based on a trilayered polyacrylamide gel e ...

2024

Carbon nanotube uptake in cyanobacteria for near-infrared imaging and enhanced bioelectricity generation in living photovoltaics

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Alessandra Antonucci, Charlotte Elisabeth Marie Roullier, Nils Schürgers, Benjamin Paul Johanès Gabriel Lambert, Alice Judith Gillen, Melania Reggente, Mohammed Mouhib

Single-walled carbon nanotubes (SWCNTs) have unique optoelectronic properties that make them suitable for applications ranging from phototherapy to imaging and sensing, but their uptake has mainly been explored in eukaryotic cells. Here the authors explore ...

2022

Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Nils Schürgers, Hanxuan Wang

Single-walled carbon nanotubes (SWCNTs) emit photostable near-infrared fluorescence that is conducive for optical glucose monitoring. Such SWCNT-based optical sensors often require the immobilization of proteins that can confer glucose selectivity and reac ...

2022

Sensing platform

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Nils Schürgers

Sensors comprising B-L-S; wherein: B is a biomolecule comprising one or more surface-exposed thiol moieties, L is a thiol reactive linking group, and S is a substrate; wherein L is covalently linked to B via the one or more surface-exposed thiol moieties. ...

2020

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Shang-Jung Wu, Nils Schürgers

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

2020

Protein Bioconjugation to Carbon Nanotubes for Near-Infrared Sensing

Vitalijs Zubkovs

Single-walled carbon nanotubes (SWCNTs) are a promising material for electrochemical and optical biosensing applications. Their small dimensions, large surface area, indefinite photostability, and remarkable electronic properties make SWCNTs an ideal trans ...

EPFL2019

Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Alessandra Antonucci, Benjamin Paul Johanès Gabriel Lambert

Fluorescence microscopy in the second near-infrared optical window (NIR-II, 1000–1350 nm) has become a technique of choice for non-invasive in vivo imaging. The deep penetration of NIR light in living tissue, as well as negligible tissue autofluorescence w ...

Nature Research2018

Mediatorless, Reversible Optical Nanosensor Enabled through Enzymatic Pocket Doping

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Esra Ahunbay, Nils Schürgers, Benjamin Paul Johanès Gabriel Lambert

This study presents a reversible, mediatorless, near-infrared glucose sensor based on glucose oxidase-wrapped SWCNTs. A combination of fluorescence, absorption, and Raman spectroscopy measurements suggest a fluorescence enhancement mechanism based on local ...

Wiley-Blackwell2017

Wide-field medium-repetition-rate multiphoton microscopy reduces photodamage of living cells

Sylvie Roke, Carlos Alberto Macias Romero, Vitalijs Zubkovs, Siyuan Wang

Demands of higher spatial and temporal resolutions in linear and nonlinear imaging keep pushing the limits of optical microscopy. We showed recently that a multiphoton microscope with 200 kHz repetition rate and wide-field illumination has a 2–3 orders of ...

2016

Probing Rotational and Translational Diffusion of Nanodoublers in Living Cells on Microsecond Time Scales

Aleksandra Radenovic, Fabrizia Dutto, Lucas Delannoy, Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Vitalijs Zubkovs

Nonlinear microscopes have seen an increase in popularity in the life sciences due to their molecular and structural specificity, high resolution, large penetration depth, and volumetric imaging capability. Nonetheless, the inherently weak optical signals ...

American Chemical Society2014

High throughput second harmonic imaging for label-free biological applications

Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Aleksandra Radenovic, Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Vitalijs Zubkovs, Orly Bagunu Tarun

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging th ...

Optical Society of America2014