Publications by Kyle Michael Douglass

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Pierre Gönczy, Virginie Hamel, Suliana Manley, Paul Guichard, Dora Mahecic, Kyle Michael Douglass, Denis Fortun, Niccolo Banterle, Khalid Ashraf Mohie Ibrahim, Davide Gambarotto

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

2020

Koehler integrator device and application thereof in a multi-focal confocal microscope

Suliana Manley, Dora Mahecic, Kyle Michael Douglass

A Koehler integrator device (10) comprises a collimating lens (11) being arranged for collimating a light field created by an incoherent or partially coherent light source, a pair of planar first and second micro-lens arrays (12, 13) being arranged for rel ...

2020

Multicolor Single-Particle Reconstruction of Protein Complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

CELL PRESS2019

EZH2 oncogenic mutations drive epigenetic, transcriptional, and structural changes within chromatin domains

Suliana Manley, Marco Mina, Kyle Michael Douglass, Elisa Oricchio, Natalya Katanayeva, Maria Christine Donaldson, Elena Battistello, Timo Henry René Rey, Stephanie Jocelyne Sungalee, Giovanni Ciriello, Daniele Tavernari

Chromatin is organized into topologically associating domains (TADs) enriched in distinct histone marks. In cancer, gain-of-function mutations in the gene encoding the enhancer of zeste homolog 2 protein (EZH2) lead to a genome-wide increase in histone-3 L ...

2019

Autonomous illumination control for localization microscopy

Suliana Manley, Baptiste Romain Samuel Ottino, Kyle Michael Douglass, Marcel Stefko

Super-resolution fluorescence microscopy improves spatial resolution, but this comes at a loss of image throughput and presents unique challenges in identifying optimal acquisition parameters. Microscope automation routines can offset these drawbacks, but ...

OPTICAL SOC AMER2018

Super-resolution microscopy to decipher multi-molecular assemblies

Suliana Manley, Paul Guichard, Kyle Michael Douglass, Christian Sieben

2018

Multicolor single-particle reconstruction of protein complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

2018

The telomeric DNA damage response occurs in the absence of chromatin decompaction

Joachim Lingner, Suliana Manley, Verena Pfeiffer, Aleksandra Vancevska, Kyle Michael Douglass

Telomeres are specialized nucleoprotein structures that protect chromosome ends from DNA damage response (DDR) and DNA rearrangements. The telomeric shelterin protein TRF2 suppresses the DDR, and this function has been attributed to its abilities to trigge ...

Cold Spring Harbor Laboratory2017

Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

Suliana Manley, Kyle Michael Douglass, Christian Sieben, Anna Archetti, Ambroise Justin Lambert

Biological processes are inherently multi-scale, and supramolecular complexes at the nanoscale determine changes at the cellular scale and beyond. Single-molecule localizationmicroscopy (SMLM)(1-3) techniques have been established as important tools for st ...

Nature Publishing Group2016

Nanoscale Spatial Organization of Chromatin in its Cellular Context, from Telomeres to Hox

Denis Duboule, Joachim Lingner, Suliana Manley, Verena Pfeiffer, Aleksandr Benke, Pierre Raymond Fabre, Aleksandra Vancevska, Kyle Michael Douglass

Cell Press2016

Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Suliana Manley, Seamus John Holden, Lina Carlini, Kyle Michael Douglass

Three-dimensional (3D) localization-based super-resolution microscopy (SR) requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, whi ...

Public Library of Science2015