Publications by Tania Hübscher

Establishment of a microwell-array-based miniaturized thymic organoid model suitable for high-throughput applications

T cell development depends critically on the thymic stroma—in particular, the diverse array of functionally distinct thymic epithelial cell (TEC) types. However, a robust in vitro thymus model mimicking the native thymus and compatible with medium-/high-th ...

2025

Thymic epithelial organoids mediate T-cell development

Matthias Lütolf, Tania Hübscher

Although the advent of organoids has opened unprecedented perspectives for basic and translational research, immune system-related organoids remain largely underdeveloped. Here, we established organoids from the thymus, the lymphoid organ responsible for T ...

2024

Bioengineering functional organoid models across scales

Tania Hübscher

The last two decades have seen the development of organoid models for many different tissues and organs. Organoids are three-dimensional organ-mimetics derived from stem or progenitor cells comprising various specialized cell types, resembling the architec ...

EPFL2024

Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo

Matthias Lütolf, Freddy Radtke, Tania Hübscher, Amber Dawn Bowler, Nicolas Antoine Vincent Broguiere, Luis Francisco Lorenzo Martin

Three-dimensional organoid culture technologies have revolutionized cancer research by allowing for more realistic and scalable reproductions of both tumour and microenvironmental structures 1-3 . This has enabled better modelling of low-complexity cancer ...

NATURE PORTFOLIO2024

Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution

Matthias Lütolf, Tania Hübscher, Sonja Giger, Giuliana Rossi

Gastruloids are three-dimensional embryonic organoids that reproduce key features of early mammalian development in vitro with unique scalability, accessibility, and spatiotemporal similarity to real embryos. Recently, we adapted the gastruloid culture con ...

Nature Portfolio2022

Developmental dynamics of the neural crest-mesenchymal axis in creating the thymic microenvironment

Matthias Lütolf, Tania Hübscher

The thymic stroma is composed of epithelial and nonepithelial cells providing separate microenvironments controlling homing, differentiation, and selection of hematopoietic precursor cells to functional T cells. Here, we explore at single-cell resolution t ...

AMER ASSOC ADVANCEMENT SCIENCE2022

3D extrusion bioprinting

Matthias Lütolf, Tania Hübscher

Three-dimensional (3D) bioprinting strategies use computer-aided processes to enable automated simultaneous spatial patterning of cells and/or biomaterials. These technologies are suitable for a broad range of biomedical applications owing to their capabil ...

SPRINGERNATURE2021