Publications by Christian Sieben

Nanoscale organization of the endogenous ASC speck

The NLRP3 inflammasome is a central component of the innate immune system. Its activation leads to for-mation of the ASC speck, a supramolecular assembly of the inflammasome adaptor protein ASC. Different models, based on ASC overexpression, have been prop ...

Cell Press2023

3D Structure From 2D Microscopy Images Using Deep Learning

Suliana Manley, Christian Sieben

Understanding the structure of a protein complex is crucial in determining its function. However, retrieving accurate 3D structures from microscopy images is highly challenging, particularly as many imaging modalities are two-dimensional. Recent advances i ...

Frontiers Media Sa2021

Nanoscale Pattern Extraction from Relative Positions of Sparse 3D Localizations

Suliana Manley, Christian Sieben

Inferring the organization of fluorescently labeled nanosized structures from single molecule localization microscopy (SMLM) data, typically obscured by stochastic noise and background, remains challenging. To overcome this, we developed a method to extrac ...

AMER CHEMICAL SOC2021

Influenza A viruses use multivalent sialic acid clusters for cell binding and receptor activation

Suliana Manley, Christian Sieben

Influenza A virus (IAV) binds its host cell using the major viral surface protein hemagglutinin (HA). HA recognizes sialic acid, a plasma membrane glycan that functions as the specific primary attachment factor (AF). Since sialic acid alone cannot fulfill ...

2020

Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans

Laurent Gillet, Christian Sieben

Virus infection is an intricate process that requires the concerted action of both viral and host cell components. Entry of viruses into cells is initiated by interactions between viral proteins and cell-surface receptors. Various cell-surface glycans func ...

ANNUAL REVIEWS2020

Multicolor Single-Particle Reconstruction of Protein Complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

CELL PRESS2019

Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

Suliana Manley, Aleksandr Benke, Beat Fierz, Andrea Callegari, David Michael Suter, Christian Sieben

Transcription factors (TFs) regulate gene expression in both prokaryotes and eukaryotes by recognizing and binding to specific DNA promoter sequences. In higher eukaryotes, it remains unclear how the duration of TF binding to DNA relates to downstream tran ...

2019

Inhibition of influenza virus activity by the bovine seminal plasma protein PDC-109

Christian Sieben

A number of viruses causing sexually transmissible diseases are transmitted via mammalian seminal plasma. Several components of seminal plasma have been shown to influence those viruses and their physiological impact. To unravel whether components of semin ...

SPRINGER2019

Super-resolution microscopy to decipher multi-molecular assemblies

Suliana Manley, Paul Guichard, Kyle Michael Douglass, Christian Sieben

2018

Multicolor single-particle reconstruction of protein complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

2018

Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

Suliana Manley, Kyle Michael Douglass, Christian Sieben, Anna Archetti, Ambroise Justin Lambert

Biological processes are inherently multi-scale, and supramolecular complexes at the nanoscale determine changes at the cellular scale and beyond. Single-molecule localizationmicroscopy (SMLM)(1-3) techniques have been established as important tools for st ...

Nature Publishing Group2016

Genetic characterization of an adapted pandemic 2009 H1N1 influenza virus that reveals improved replication rates in human lung epithelial cells

Christian Sieben

The 2009 influenza pandemic originated from a swine-origin H1N1 virus, which, although less pathogenic than anticipated, may acquire additional virulence-associated mutations in the future. To estimate the potential risk, we sequentially passaged the isola ...

Academic Press Inc Elsevier Science2016

Viral RNA Degradation and Diffusion Act as a Bottleneck for the Influenza A Virus Infection Efficiency

Christian Sieben

After endocytic uptake, influenza viruses transit early endosomal compartments and eventually reach late endosomes. There, the viral glycoprotein hemagglutinin (HA) triggers fusion between endosomal and viral membrane, a critical step that leads to release ...

Public Library Science2016

Influenza a Viruses use Multivalent Sialic Acid Clusters for Cell Binding and Receptor Activation

Suliana Manley, Christian Sieben

Cell Press2016

Heterogeneity of AMPA receptor trafficking and molecular interactions revealed by superresolution analysis of live cell imaging

Suliana Manley, Christian Sieben

Simultaneous tracking of many thousands of individual particles in live cells is possible now with the advent of high-density superresolution imaging methods. We present an approach to extract local biophysical properties of cell-particle interaction from ...

Natl Acad Sciences2012