Publications by Samuel Rommelaere

An updated proteomic analysis of Drosophila haemolymph after bacterial infection

Bruno Lemaitre, Fanny Schüpfer, Samuel Rommelaere

Using an in-depth Mass Spectrometry-based proteomics approach, we provide a comprehensive characterization of the hemolymphatic proteome of adult flies upon bacterial infection. We detected and quantified changes in abundance of several known immune regula ...

2025

The secreted Nimrod protein NimB1 negatively regulates early steps of apoptotic cell phagocytosis in Drosophila

Bruno Lemaitre, Samuel Rommelaere, Bianca Marie Petrignani

Efferocytosis, the efficient clearance of apoptotic cells (ACs) by phagocytes, is vital for maintaining tissue homeostasis. Here, we reveal the role of the secreted protein NimB1 in reducing apoptotic cell recognition and binding in the early stages of eff ...

2025

A humoral stress response protects Drosophila tissues from antimicrobial peptides

Jean Philippe Boquete, Bruno Lemaitre, Matteo Dal Peraro, Alexia Laurie Carboni, Samuel Rommelaere, Chan Cao, Fernando Augusto Teixeira Pinto Meireles

An efficient immune system must provide protection against a broad range of pathogens without causing excessive collateral tissue damage. While immune effectors have been well characterized, we know less about the resilience mechanisms protecting the host ...

Elsevier BV2024

Protein target highlights in CASP15: Analysis of models by structure providers

Bruno Lemaitre, Luciano Andres Abriata, Kuan-Lin Wu, Samuel Rommelaere, Han Xiao

We present an in-depth analysis of selected CASP15 targets, focusing on their biological and functional significance. The authors of the structures identify and discuss key protein features and evaluate how effectively these aspects were captured in the su ...

WILEY2023

CERT1 mutations perturb human development by disrupting sphingolipid homeostasis

Paolo De Los Rios, Bruno Lemaitre, Evelyne Ruchti, Sylvia Ho, Anthony Vocat, Samuel Rommelaere, Greta Limoni, Stefano Zamuner, Giovanni D'Angelo, Laura Capolupo, Charlotte Julie Caroline Gehin, Huixin Guo

Neural differentiation, synaptic transmission, and action potential propagation depend on membrane sphingolipids, whose metabolism is tightly regulated. Mutations in the ceramide transporter CERT (CERT1), which is involved in sphingolipid biosynthesis, are ...

American Society for Clinical Investigation2023

Disproportionate investment in Spiralin B production limits in-host growth and favors the vertical transmission of Spiroplasma insect endosymbionts

Jean Philippe Boquete, Bruno Lemaitre, Fanny Schüpfer, Samuel Rommelaere, Florent François Masson

Insects frequently harbor endosymbionts, which are bacteria housed within host tissues. These associations are stably maintained over evolutionary timescales through vertical transmission of endosymbionts from host mothers to their offspring. Some endosymb ...

National Academy of Sciences2022

A secreted factor NimrodB4 promotes the elimination of apoptotic corpses by phagocytes in Drosophila

Bruno Lemaitre, Samuel Rommelaere, Elodie Julie Virginie Ramond, Bianca Marie Petrignani, Florent François Masson

Programmed cell death plays a fundamental role in development and tissue homeostasis. Professional and non-professional phagocytes achieve the proper recognition, uptake, and degradation of apoptotic cells, a process called efferocytosis. Failure in effero ...

2021

Dual proteomics of Drosophila melanogaster hemolymph infected with the heritable endosymbiont Spiroplasma poulsonii

Bruno Lemaitre, Fanny Schüpfer, Samuel Rommelaere, Florent François Masson, Alice Marra

Insects are frequently infected with heritable bacterial endosymbionts. Endosymbionts have a dramatic impact on their host physiology and evolution. Their tissue distribution is variable with some species being housed intracellularly, some extracellularly ...

2021

Renal Purge of Hemolymphatic Lipids Prevents the Accumulation of ROS-Induced Inflammatory Oxidized Lipids and Protects Drosophila from Tissue Damage

Bruno Lemaitre, Samuel Rommelaere, Xiaoxue Li

Animals require complex metabolic and physiological adaptations to maintain the function of vital organs in response to environmental stresses and infection. Here, we found that infection or injury in Drosophila induced the excretion of hemolymphatic lipid ...

2020

Blind killing of both male and female Drosophila embryos by a natural variant of the endosymbiotic bacterium Spiroplasma poulsonii

Bruno Lemaitre, Juan Camilo Paredes Escobar, Mario Gonzalo Garcia Arraez, Samuel Rommelaere, Florent François Masson

Spiroplasma poulsonii is a vertically transmitted endosymbiont of Drosophila melanogaster that causes male-killing, that is the death of infected male embryos during embryogenesis. Here, we report a natural variant of S. poulsonii that is efficiently verti ...

WILEY2020

The Exchangeable Apolipoprotein Nplp2 Sustains Lipid Flow and Heat Acclimation in Drosophila

Jean Philippe Boquete, Bruno Lemaitre, Jérémie Piton, Samuel Rommelaere

In ectotherms, increased ambient temperature requires the organism to consume substantial amounts of energy to sustain a higher metabolic rate, prevent cellular damage, and respond to heat stress. Here, we identify a heat-inducible apolipoprotein required ...

2019

Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps

Bruno Lemaitre, Anna Dostalova, Samuel Rommelaere

Background: Members of the thioester-containing protein (TEP) family contribute to host defence in both insects and mammals. However, their role in the immune response of Drosophila is elusive. In this study, we address the role of TEPs in Drosophila immun ...

2017