Publications by Chiara Toniolo

Open-source microscope add-on for structured illumination microscopy

Matthias Lütolf, Theo Lasser, Aleksandra Radenovic, John McKinney, Georg Fantner, Olivier François Venzin, Mélanie Thérèse Marie Hannebelle, Tomas Lukes, Samuel Mendes Leitão, Antonius Chrisnandy, Chiara Toniolo, Andrew Charles Oates, Prabhu Prasad Swain, Nathan Cyprien Arsène Ronceray

Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to ...

Nature Portfolio2024

Mechanical morphotype switching as an adaptive response in mycobacteria

John McKinney, Georg Fantner, Chiara Toniolo

Invading microbes face a myriad of cidal mechanisms of phagocytes that inflict physical damage to microbial structures. How intracellular bacterial pathogens adapt to these stresses is not fully understood. Here, we report the discovery of a virulence mech ...

Amer Assoc Advancement Science2024

Uptake-independent killing of macrophages by extracellular Mycobacterium tuberculosis aggregates

John McKinney, Neeraj Dhar, Chiara Toniolo

Mycobacterium tuberculosis (Mtb) infection is initiated by inhalation of bacteria into lung alveoli, where they are phagocytosed by resident macrophages. Intracellular Mtb replication induces the death of the infected macrophages and the release of bacteri ...

WILEY2023

Preexisting Heterogeneity of Inducible Nitric Oxide Synthase Expression Drives Differential Growth of Mycobacterium tuberculosis in Macrophages

John McKinney, Ophélie Rutschmann, Chiara Toniolo

Mycobacterium tuberculosis infection is initiated by the inhalation and implantation of bacteria in the lung alveoli, where they are phagocytosed by macrophages. Even a single bacterium may be sufficient to initiate infection. Thereafter, the clinical outc ...

AMER SOC MICROBIOLOGY2022

A biphasic growth model for cell pole elongation in mycobacteria

Joëlle Xiao Yuan Ven, John McKinney, Gaëlle Madeleine Vuaridel, Georg Fantner, Mélanie Thérèse Marie Hannebelle, Haig Alexander Eskandarian, Chiara Toniolo

Mycobacteria grow by inserting new cell wall material in discrete zones at the cell poles. This pattern implies that polar growth zones must be assembled de novo at each division, but the mechanisms that control the initiation of new pole growth are unknow ...

Nature Research2020

Do chance encounters between heterogeneous cells shape the outcome of tuberculosis infections?

John McKinney, Ophélie Rutschmann, Chiara Toniolo

The sum of all of the interactions between single bacteria and host cells determines if an infection is cleared, controlled, or progresses at the whole host-organism level. These individual interactions have independent trajectories defined by diverse and ...

2020

Time-Lapse Atomic Force Microscopy Reveals New End Take Off (Neto) Dynamics in Mycobacteria

Joëlle Xiao Yuan Ven, John McKinney, Georg Fantner, Adrian Pascal Nievergelt, Mélanie Thérèse Marie Hannebelle, Haig Alexander Eskandarian, Chiara Toniolo

CELL PRESS2019

A microfluidic cell-trapping device to study dynamic host-microbe interactions at the single-cell level

Matthieu Jean-Hubert Delincé, John McKinney, Chiara Toniolo

Single-cell imaging of host-microbe interactions over time is impeded by cellular motility because the cells under scrutiny tend to migrate out of the imaging field. To overcome this technical challenge, we developed a microfluidic platform for imaging hun ...

ELSEVIER ACADEMIC PRESS INC2018