Publications by Chan Cao | EPFL Graph Search

Uses of aerolysin nanopores

Hilal Lashuel, Matteo Dal Peraro, Chan Cao

The invention relates to use of an aerolysin nanopore for the discrimination of different protein isoforms bearing different post-translational modifications (PTMs) and to methods for detecting adverse medical conditions associated with the presence of pep ...

2025

Mutant aerolysin and uses thereof

Matteo Dal Peraro, Chan Cao

Provided herein are aerolysin polypeptides and/or mutant aerolysin monomers comprising modified amino acid sequences that could have improved substrate analyte, such as (poly)nucleotide and peptide, improved reading properties such as enhanced substrate an ...

2024

A humoral stress response protects Drosophila tissues from antimicrobial peptides

Jean Philippe Boquete, Bruno Lemaitre, Matteo Dal Peraro, Alexia Laurie Carboni, Samuel Rommelaere, Chan Cao, Fernando Augusto Teixeira Pinto Meireles

An efficient immune system must provide protection against a broad range of pathogens without causing excessive collateral tissue damage. While immune effectors have been well characterized, we know less about the resilience mechanisms protecting the host ...

Elsevier BV2024

Single-molecule evidence of Entropic Pulling by Hsp70 chaperones

Paolo De Los Rios, Chan Cao, Mathieu Rebeaud

Hsp70 chaperones are central components of the cellular network that ensures the structural quality of proteins. Despite crucial roles in processes such as protein disaggregation and protein translocation into organelles, their physical mechanism of action ...

Nature Research2024

Deep Learning-Assisted Single-Molecule Detection of Protein Post-translational Modifications with a Biological Nanopore

Hilal Lashuel, Anass Chiki, Juan Francisco Bada Juarez, Matteo Dal Peraro, Lucien Fabrice Krapp, Chan Cao, Simon Finn Mayer, Pedro José Santana Ribeiro Magalhães

Protein post-translational modifications (PTMs) play a crucial role in countless biological processes, profoundly modulating protein properties on both spatial and temporal scales. Protein PTMs have also emerged as reliable biomarkers for several diseases. ...

Amer Chemical Soc2023

Systems and methods for digital information decoding and data storage in hybrid macromolecules

Aleksandra Radenovic, Matteo Dal Peraro, Lucien Fabrice Krapp, Chan Cao

Systems and methods for data storage and readout using hybrid nucleic acid- polymeric molecules are herein disclosed. A molecular data storage medium is presented comprising a header and a footer, each comprising or consisting of at least one unit of a fir ...

2022

Bacterial nanopores open the future of data storage

Aleksandra Radenovic, Matteo Dal Peraro, Lucien Fabrice Krapp, Chan Cao, Alissa Agerova

In the era of "big data" finding solutions for data storage alternative to those based on silicon or magnetic tapes is an urgent need for our society. The development of polymers that can store information at the molecular level has opened up new opportuni ...

IEEE2022

Biological nanopores for single-molecule sensing

Matteo Dal Peraro, Chan Cao, Simon Finn Mayer

Evolution has found countless ways to transport material across cells and cellular compartments separated by membranes. Protein assemblies are the cornerstone for the formation of channels and pores that enable this regulated passage of molecules in and ou ...

2022

Ultrasensitive Label-Free Detection of Protein–Membrane Interaction Exemplified by Toxin-Liposome Insertion

Mircea Ioan Iacovache, Matteo Dal Peraro, Sylvie Roke, Halil Ibrahim Okur, Chan Cao, Tereza Schönfeldová

Measuring the high-affinity binding of proteins to liposome membranes remains a challenge. Here, we show an ultrasensitive and direct detection of protein binding to liposome membranes using high throughput second harmonic scattering (SHS). Perfringolysin ...

2022

Mutant aerolysin and uses thereof

Matteo Dal Peraro, Chan Cao

Aerolysin polypeptides and/or mutant aerolysin monomers include modified amino acid sequences that could have improved substrate analyte, such as (poly)nucleotide and peptide, improved reading properties such as enhanced substrate analyte capture and impro ...

2022

The emerging landscape of single-molecule protein sequencing technologies

Michael Mayer, Sonja Schmid, Chan Cao

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe ...

2021

Decoding Digital Information Stored in Polymer by Nanopore

Aleksandra Radenovic, Matteo Dal Peraro, Lucien Fabrice Krapp, Chan Cao

CELL PRESS2021

Single-molecule studies of amyloid proteins: from biophysical properties to diagnostic perspectives

Chan Cao

In neurodegenerative diseases, a wide range of amyloid proteins or peptides such as amyloid-beta and alpha-synuclein fail to keep native functional conformations, followed by misfolding and self-assembling into a diverse array of aggregates. The aggregates ...

CAMBRIDGE UNIV PRESS2020

Aerolysin nanopores decode digital information stored in tailored macromolecular analytes

Aleksandra Radenovic, Matteo Dal Peraro, Lucien Fabrice Krapp, Nuria Cirauqui Diaz, Chan Cao

Digital data storage is a growing need for our society and finding alternative solutions than those based on silicon or magnetic tapes is a challenge in the era of “big data.” The recent development of polymers that can store information at the molecular l ...

2020

Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores

Aleksandra Radenovic, Matteo Dal Peraro, Alice Duperrex, Nuria Cirauqui Diaz, Chan Cao, Elena Buglakova

Nanopore sensing is a powerful single-molecule approach for the detection of biomolecules. Recent studies have demonstrated that aerolysin is a promising candidate to improve the accuracy of DNA sequencing and to develop novel single-molecule proteomic str ...

Nature Research2019

Mapping the sensing spots of aerolysin for single oligonucleotides analysis

Matteo Dal Peraro, Nuria Cirauqui Diaz, Chan Cao

Nature Publishing Group2018