Publications by Nicolas Antoine Vincent Broguiere

Accessible homeostatic gastric organoids reveal secondary cell type-specific host-pathogen interactions in Helicobacter pylori infections

Matthias Lütolf, Moritz Hofer, Nicolas Antoine Vincent Broguiere

Despite the high prevalence of gastric diseases like gastric cancer and peptic ulcer disease attributed to Helicobacter pylori infections, there is still only a limited understanding of the underlying mechanisms. Existing in vitro models are either two-dim ...

NATURE PORTFOLIO2025

Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids

Matthias Lütolf, Damien Saugy, Nathalie Brandenberg, François Réal Rivest, Sylke Höhnel, Krisztian Homicsko, Nicolas Antoine Vincent Broguiere, Devanjali Dutta

Immunotherapy has emerged as a new standard of care for certain cancer patients with specific cellular and molecular makeups. However, there is still an unmet need for ex vivo models able to readily assess the effectiveness of immunotherapeutic treatments ...

2024

Bioengineered human colon organoids with in vivo-like cellular complexity and function

Nicolas Antoine Vincent Broguiere

Organoids and organs-on-a-chip have emerged as powerful tools for modeling human gut physiology and disease in vitro. Although physiologically relevant, these systems often lack the environmental milieu, spatial organization, cell type diversity, and matur ...

2024

Patient-derived mini-colons enable long-term modeling of tumor–microenvironment complexity

Matthias Lütolf, Nicolas Antoine Vincent Broguiere

Existing organoid models fall short of fully capturing the complexity of cancer because they lack sufficient multicellular diversity, tissue-level organization, biological durability and experimental flexibility. Thus, many multifactorial cancer processes, ...

2024

Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo

Matthias Lütolf, Freddy Radtke, Tania Hübscher, Amber Dawn Bowler, Nicolas Antoine Vincent Broguiere, Luis Francisco Lorenzo Martin

Three-dimensional organoid culture technologies have revolutionized cancer research by allowing for more realistic and scalable reproductions of both tumour and microenvironmental structures 1-3 . This has enabled better modelling of low-complexity cancer ...

NATURE PORTFOLIO2024

Nanoplasmonic Single-Tumoroid Microarray for Real-Time Secretion Analysis

Hatice Altug, Yen-Cheng Liu, Nicolas Antoine Vincent Broguiere, Saeid Ansaryan, Luis Francisco Lorenzo Martin

Organoid tumor models have emerged as a powerful tool in the fields of biology and medicine as such 3D structures grown from tumor cells recapitulate better tumor characteristics, making these tumoroids unique for personalized cancer research. Assessment o ...

2024

Dynamic Hydrogel Design For Spatiotemporal Control Of Morphogenesis

Matthias Lütolf, Antonius Chrisnandy, Nicolas Antoine Vincent Broguiere

MARY ANN LIEBERT, INC2023

A New Approach to Generate Gastruloids to Develop Anterior Neural Tissues

Matthias Lütolf, Nicolas Antoine Vincent Broguiere

Embryonic development is a complex process integrating cell fate decisions and morphogenesis in a spatiotemporally controlled manner. Previous studies with model organisms laid the foundation of our knowledge on post-implantation development; however, stud ...

BIO-PROTOCOL2023

Next-generation cancer organoids

Matthias Lütolf, Nicolas Antoine Vincent Broguiere

Organotypic models of patient-specific tumours are revolutionizing our understanding of cancer heterogeneity and its implications for personalized medicine. These advancements are, in part, attributed to the ability of organoid models to stably preserve ge ...

NATURE PORTFOLIO2022

Dissecting mechanisms of chamber-specific cardiac differentiation and its perturbation following retinoic acid exposure

Matthias Lütolf, Giuliana Rossi, Nicolas Antoine Vincent Broguiere

The specification of distinct cardiac lineages occurs before chamber formation and acquisition of bona fide atrial or ventricular identity. However, the mechanisms underlying these early specification events remain poorly understood. Here, we performed sin ...

COMPANY BIOLOGISTS LTD2022

Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis

Matthias Lütolf, Delphine Lorraine Perle Blondel, Saba Rezakhani, Antonius Chrisnandy, Nicolas Antoine Vincent Broguiere

Epithelial organoids are most efficiently grown from mouse-tumour-derived, reconstituted extracellular matrix hydrogels, whose poorly defined composition, batch-to-batch variability and immunogenicity limit clinical applications. Efforts to replace such il ...

NATURE PORTFOLIO2022

Capturing Cardiogenesis in Gastruloids

Matthias Lütolf, Romain Guiet, Mehmet Ugur Girgin, Giuliana Rossi, Andrea Boni, Nicolas Antoine Vincent Broguiere

Organoids are powerful models for studying tissue development, physiology, and disease. However, current culture systems disrupt the inductive tissue-tissue interactions needed for the complex morphogenetic processes of native organogenesis. Here, we show ...

CELL PRESS2021

Bioprinting of Cartilaginous Auricular Constructs Utilizing an Enzymatically Crosslinkable Bioink

Philipp Fisch, Nicolas Antoine Vincent Broguiere

Bioprinting of functional tissues could overcome tissue shortages and allow a more rapid response for treatments. However, despite recent progress in bioprinting, and its outstanding ability to position cells and biomaterials in a precise 3D manner, its su ...

WILEY-V C H VERLAG GMBH2021

Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

Matthias Lütolf, Mehmet Ugur Girgin, Nicolas Antoine Vincent Broguiere

When stimulated with a pulse from an exogenous WNT pathway activator, small aggregates of mouse embryonic stem cells (ESCs) can undergo embryo-like axial morphogenesis and patterning along the three major body axes. However, these structures, called gastru ...

2021

Bioengineered embryoids mimic post-implantation development in vitro

Matthias Lütolf, Mehmet Ugur Girgin, Nicolas Antoine Vincent Broguiere

The difficulty of studying post-implantation development in mammals has sparked a flurry of activity to develop in vitro models, termed embryoids, based on self-organizing pluripotent stem cells. Previous approaches to derive embryoids either lack the phys ...

Nature Portfolio2021

PEG/HA Hybrid Hydrogels for Biologically and Mechanically Tailorable Bone Marrow Organoids

Martin Ehrbar, Queralt Vallmajó Martín, Nicolas Antoine Vincent Broguiere

Bone marrow (BM) organoids provide powerful tools to study the vital interplay between the BM microenvironment and resident cells. Current biomaterials, however, are limited in terms of versatility for independently studying the biochemical and biophysical ...

WILEY-V C H VERLAG GMBH2020

Morphogenesis Guided by 3D Patterning of Growth Factors in Biological Matrices

Matthias Lütolf, Nicolas Antoine Vincent Broguiere, Riccardo Rizzo

Three-dimensional (3D) control over the placement of bioactive cues is fundamental to understand cell guidance and develop engineered tissues. Two-photon patterning (2PP) provides such placement at micro- to millimeter scale, but nonspecific interactions b ...

Wiley-V C H Verlag Gmbh2020

Homeostatic mini-intestines through scaffold-guided organoid morphogenesis

Matthias Lütolf, Yoji Tabata, Nathalie Brandenberg, Nikolche Gjorevski, Mikhail Nikolaev, Olga Mitrofanova, Nicolas Antoine Vincent Broguiere, Bilge Sen Elçi, Devanjali Dutta

Epithelial organoids, such as those derived from stem cells of the intestine, have great potential for modelling tissue and disease biology(1-4). However, the approaches that are used at present to derive these organoids in three-dimensional matrices(5,6)r ...

NATURE PUBLISHING GROUP2020