Publications by Leo Scheller | EPFL Graph Search

Targeting protein-ligand neosurfaces with a generalizable deep learning tool

Luc Reymond, Sebastian Maerkl, Philippe Schwaller, Sandrine Madeleine Suzanne Georgeon, Anthony Marchand, Bruno Emanuel Ferreira De Sousa Correia, Pablo Gainza Cirauqui, Leo Scheller, Stephen Michael Buckley, Arne Schneuing, Martin Pacesa

Molecular recognition events between proteins drive biological processes in living systems1. However, higher levels of mechanistic regulation have emerged, in which protein-protein interactions are conditioned to small molecules2, 3, 4-5. Despite recent ad ...

NATURE PORTFOLIO2025

Dual ON/OFF-switch chimeric antigen receptor controlled by two clinically approved drugs

Sandrine Madeleine Suzanne Georgeon, Bruno Emanuel Ferreira De Sousa Correia, Sailan Shui, Leo Scheller

The ability to remotely control the activity of chimeric antigen receptors (CARs) with small molecules can improve the safety and efficacy of gene-modified T cells. Split ON- or OFF-switch CARs involve the dissociation of tumor–antigen binding from T cell ...

National Academy of Sciences2024

Rational Design of Chemically Controlled Antibodies and Protein Therapeutics

Sandrine Madeleine Suzanne Georgeon, Anthony Marchand, Bruno Emanuel Ferreira De Sousa Correia, Pablo Gainza Cirauqui, Li Tang, Sailan Shui, Stéphane Rosset, Lucia Bonati, Leo Scheller

Protein-based therapeutics, such as monoclonal antibodies and cytokines, are important therapies for various pathophysiological conditions such as oncology, autoimmune disorders, and viral infections. However, the wide application of such protein therapeut ...

2023

Rational design of small-molecule responsive protein switches

Bruno Emanuel Ferreira De Sousa Correia, Sailan Shui, Leo Scheller, Stephen Michael Buckley

Small-molecule responsive protein switches are powerful tools for controlling cellular processes. These switches are designed to respond rapidly and specifically to their inducer. They have been used in numerous applications, including the regulation of ge ...

2023

Protein-based bandpass filters for controlling cellular signaling with chemical inputs

Bruno Emanuel Ferreira De Sousa Correia, Sailan Shui, Leo Scheller

Biological signal processing is vital for cellular function. Similar to electronic circuits, cells process signals via integrated mechanisms. In electronics, bandpass filters transmit frequencies with defined ranges, but protein-based counterparts for cont ...

Nature Portfolio2023

Programmable DARPin-based receptors for the detection of thrombotic markers

Haifeng Ye, Leo Scheller, Yujia Wang

Cellular therapies remain constrained by the limited availability of sensors for disease markers. Here we present an integrated target-to-receptor pipeline for constructing a customizable advanced modular bispecific extracellular receptor (AMBER) that comb ...

NATURE PORTFOLIO2022

Computational design of bioactive protein switches with multi-logics for cell-based therapeutics

Bruno Emanuel Ferreira De Sousa Correia, Che Yang, Pablo Gainza Cirauqui, Sailan Shui, Leo Scheller

WILEY2021

Bottom-up de novo design of functional proteins with complex structural features

Sandrine Madeleine Suzanne Georgeon, Luciano Andres Abriata, Bruno Emanuel Ferreira De Sousa Correia, Jaume Bonet Martinez, Fabian Sesterhenn, Che Yang, Stéphane Rosset, Eva Anna van Aalen, Leo Scheller

De novo protein design has enabled the creation of new protein structures. However, the design of functional proteins has proved challenging, in part due to the difficulty of transplanting structurally complex functional sites to available protein structur ...

2021

Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells

Karl Gademann, Simon Sieber, Leo Scheller

We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian c ...

2020

Calcium-Mediated Liposome Fusion to Engineer Giant Lipid Vesicles with Cytosolic Proteins and Reconstituted Mammalian Proteins

Leo Scheller

Giant unilamellar lipid vesicles (GUVs) are widely used as model membrane systems and provide an excellent basis to construct artificial cells. To construct more sophisticated artificial cells, proteins-in particular membrane proteins-need to be incorporat ...

2020

Phosphoregulated orthogonal signal transduction in mammalian cells

Maysam Mansouri, Leo Scheller

Orthogonal tools for controlling protein function by post-translational modifications open up new possibilities for protein circuit engineering in synthetic biology. Phosphoregulation is a key mechanism of signal processing in all kingdoms of life, but too ...

Nature Research2020