Publication

A Ca2+ ion selective electrode biosensor in microfluidics to monitor hepatocyte’s activities

Philippe Renaud, Harald Van Lintel, Robert Meissner
2010
Conference Papers

Abstract

Ca2+ plays a crucial role in cell communication. For example, periodical changes in Ca2+ concentration act as cell sig- nals. Intracellular Ca2+ signals have been measured by Ca2+ sensitive fluorescent dye [1]. In this study, extracellular Ca2+ variation was monitored by a potentiometry using a solid-state type of Ca2+ Ion Selective Electrode (ISE). Ca2+ ISE had high sensitivity detecting down to 10-10M of calcium concentration, as well as high selectivity against K+. After KCl sti- mulus of cells in a microchannel, we observed an oscillating increase in Ca2+ concentration, associated with a periodic oscillation of cell activity (Hepatocyte, Hep2G).!

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https://infoscience.epfl.ch/entities/publication/0a71a9ce-8a8c-4dea-9980-d85e61f44ecc

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Philippe Renaud, Harald Van Lintel, Robert Meissner
2010
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/0a71a9ce-8a8c-4dea-9980-d85e61f44ecc

About this result

Related concepts (16)

Calcium in biology

Calcium ions (Ca2+) contribute to the physiology and biochemistry of organisms' cells. They play an important role in signal transduction pathways, where they act as a second messenger, in neurotransmitter release from neurons, in contraction of all muscle cell types, and in fertilization. Many enzymes require calcium ions as a cofactor, including several of the coagulation factors. Extracellular calcium is also important for maintaining the potential difference across excitable cell membranes, as well as proper bone formation.

Calcium signaling

Calcium signaling is the use of calcium ions (Ca2+) to communicate and drive intracellular processes often as a step in signal transduction. Ca2+ is important for cellular signalling, for once it enters the cytosol of the cytoplasm it exerts allosteric regulatory effects on many enzymes and proteins. Ca2+ can act in signal transduction resulting from activation of ion channels or as a second messenger caused by indirect signal transduction pathways such as G protein-coupled receptors.

Cell signaling

In biology, cell signaling (cell signalling in British English) or cell communication is the ability of a cell to receive, process, and transmit signals with its environment and with itself. Cell signaling is a fundamental property of all cellular life in prokaryotes and eukaryotes. Signals that originate from outside a cell (or extracellular signals) can be physical agents like mechanical pressure, voltage, temperature, light, or chemical signals (e.g., small molecules, peptides, or gas).

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