Publication

Electrospray Micromixer Chip for On-Line Derivatization and Kinetic Studies

Hubert Girault, Niels Erik Lion, Loïc Dayon, Mélanie Abonnenc, Brice Perruche
2008
Journal Articles

Abstract

An electrospray microchip for mass spectrometry comprising an integrated passive mixer to carry out on-chip chemical derivatizations is described. The microchip fabricated using UV-photoablation is composed of two microchannels linked together by a liquid junction. Downstream of this liquid junction, a mixing unit made of parallel oblique grooves is integrated to the microchannel in order to create flow perturbations. Several mixer designs are evaluated. The mixer efficiency is investigated both by fluorescence study and mass spectrometric monitoring of the tagging reaction of cysteinyl peptides with 1,4-benzoquinone. The comparisons with a microchip without a mixing unit and a kinetic model are used to assess the efficiency of the mixer showing tagging kinetics close to that of bulk reactions in an ideally mixed reactor. As an ultimate application, the electrospray micromixer is implemented in a LC-MS workflow. Online derivatization of albumin tryptic peptides after a reversed-phase separation and counting of their cysteines drastically enhance the protein identification.

Official source

https://infoscience.epfl.ch/entities/publication/0cb400df-620b-435f-9e12-4664358eb1d6

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Hubert Girault, Niels Erik Lion, Loïc Dayon, Mélanie Abonnenc, Brice Perruche
2008
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/0cb400df-620b-435f-9e12-4664358eb1d6

About this result

Related concepts (31)

Mixing (process engineering)

In industrial process engineering, mixing is a unit operation that involves manipulation of a heterogeneous physical system with the intent to make it more homogeneous. Familiar examples include pumping of the water in a swimming pool to homogenize the water temperature, and the stirring of pancake batter to eliminate lumps (deagglomeration). Mixing is performed to allow heat and/or mass transfer to occur between one or more streams, components or phases. Modern industrial processing almost always involves some form of mixing.

Mixing console

A mixing console or mixing desk is an electronic device for mixing audio signals, used in sound recording and reproduction and sound reinforcement systems. Inputs to the console include microphones, signals from electric or electronic instruments, or recorded sounds. Mixers may control analog or digital signals. The modified signals are summed to produce the combined output signals, which can then be broadcast, amplified through a sound reinforcement system or recorded.

Audio mixing (recorded music)

In sound recording and reproduction, audio mixing is the process of optimizing and combining multitrack recordings into a final mono, stereo or surround sound product. In the process of combining the separate tracks, their relative levels are adjusted and balanced and various processes such as equalization and compression are commonly applied to individual tracks, groups of tracks, and the overall mix. In stereo and surround sound mixing, the placement of the tracks within the stereo (or surround) field are adjusted and balanced.

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