Publications related to Engineering Stem Cell Self-organization to Build Better Organoids

Accessible and highly observable gastrointestinal organoid model systems for studying host-pathogen interactions

Traditional cell cultures have long been fundamental to biological research, offering an alternative to animal models burdened by ethical constraints and procedural intricacies, often lacking relevance to human physiology and disease. Moreover, their inabi ...

EPFL2024

The impact of different methods of increasing the intensity of compassion in engineering ethics cases

Roland John Tormey, Nihat Kotluk

Despite the growing interest in emotions in engineering education, empirical research on incorporating them into engineering ethics education is limited. Therefore, we designed this experimental study to assess how different methods for integrating compass ...

2024

Bioengineering functional organoid models across scales

Tania Hübscher

The last two decades have seen the development of organoid models for many different tissues and organs. Organoids are three-dimensional organ-mimetics derived from stem or progenitor cells comprising various specialized cell types, resembling the architec ...

EPFL2024

“But of course i’m going to look happy” or “He needed to know I was angry”? Comparing use of emotional labour in teamwork in engineering and hospitality students

Roland John Tormey, Nihat Kotluk

Being able to work effectively in a team is a vital professional skill but how do students in different disciplines, engineering and hospitality, display their emotions when working together? We investigated their self-reported use of emotional labour stra ...

EAPRIL - European Association for Practitioner Research on Improving Learning2024

Engineering hydrogel microenvironments for epithelial organoid culture

Antonius Chrisnandy

Organoids, miniature tissues generated from self-organizing stem cells within three-dimensional (3D) extracellular matrices (ECM), have opened up exciting possibilities for in vitro studies of complex physiological processes. A key factor in the success of ...

EPFL2024

Next-Generation Modeling of Cancer Using Organoids

Wouter Richard Karthaus, Jillian Rose Love

In the last decade, organoid technology has become a cornerstone in cancer research. Organoids are long-term primary cell cultures, usually of epithelial origin, grown in a three-dimensional (3D) protein matrix and a fully defined medium. Organoids can be ...

Cold Spring Harbor Lab Press, Publications Dept2024

Deciphering the nature of cell state transitions in single cells using quantitative modeling of temporal dynamics

Alex Russell Lederer

Cells are the smallest operational units of living systems. Through synthesis of various biomolecules and exchange of signals with the environment, cells tightly regulate their composition to realize a specific functional state. The transformation of a cel ...

EPFL2024

Clinically compliant cryopreservation of differentiated retinal pigment epithelial cells

Cheng Zhao, Gioele La Manno, Alex Russell Lederer

Background aims: Age-related macular degeneration (AMD) is the most common cause of blindness in elderly patients within developed countries, affecting more than 190 million worldwide. In AMD, the retinal pigment epithelial (RPE) cell layer progressively d ...

Elsevier Sci Ltd2024

Characterization of the gut-bone marrow axis through bile acid signaling

Alejandro Alonso Calleja

Communication between the intestine and other organs such as the lungs, brain or bones is mediated by several metabolites, like short-chain fatty acids or bile acids, that relay information about nutritional and microbiota status. Bile acids are endogenous ...

EPFL2024

Multimodal characterization of murine gastruloid development

Matthias Lütolf, Raphaël Ortiz, Stefano Davide Vianello, Alexandre Gauthier Aurèle Mayran

Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development ...

CELL PRESS2023

Bioengineering human mini-intestines with in vivo-like complexity and function

Olga Mitrofanova

Biological research heavily relies on the use of animal models, which has made it difficult to answer specific questions about human biology and disease. However, with the advent of human organoids - miniature versions of tissues generated in 3D human stem ...

EPFL2023

Neuronal migration prevents spatial competition in retinal morphogenesis

Martin Weigert

The concomitant occurrence of tissue growth and organization is a hallmark of organismal development(1-3). This often means that proliferating and differentiating cells are found at the same time in a continuously changing tissue environment. How cells ada ...

NATURE PORTFOLIO2023

Programming of Multicellular Patterning with Mechano-Chemically Microstructured Cell Niches

Pierre Osteil

Multicellular patterning of stem-cell-derived tissue models is commonly achieved via self-organizing activities triggered by exogenous morphogenetic stimuli. However, such tissue models are prone to stochastic behavior, limiting the reproducibility of cell ...

WILEY2023

Educating Engineering Students to Address Bias and Discrimination Within Their Project Teams

Siara Ruth Isaac, Roland John Tormey, Nihat Kotluk

What training should engineering students receive to enable them to contribute to reducing bias, discrimination and the persistent lack of diversity in engineering? Collaboration is central to professional engineering work and, consequently, teamwork and g ...

2023

Engineered substrates incapable of induction of chondrogenic differentiation compared to the chondrocyte imprinted substrates

Philippe Renaud, Shahin Bonakdar, Leila Montazeri, Zahrasadat Ghazali

It is well established that surface topography can affect cell functions. However, finding a reproducible and reliable method for regulating stem cell behavior is still under investigation. It has been shown that cell imprinted substrates contain micro- an ...

IOP Publishing Ltd2023

Self-sustained dynamics and forced resonant oscillations in flows: cross-junction jets and sloshing liquids

Alessandro Bongarzone

In engineering, oscillatory instabilities and resonances are often considered undesirable flow features and measures are taken to avoid them. This may include avoiding certain parametric regions or implementing control and mitigation strategies. However, t ...

EPFL2023

Making Bridges Sustainable

Eugen Brühwiler

Lack of incentives mainly explains why the implementation of novel methods and technologies in bridge engineering practice is generally postponed by the engineering community despite the urgent need to advance also bridge engineering to meet the objectives ...

Taylor and Francis / CRC Press2023

Deciphering the molecular events leading to the global restoration of the chromatin landscape during mitotic exit

Silja Michelle Placzek

Two fundamental properties of embryonic stem cells (ESCs) are their ability to self-renew and differentiate into all somatic cell types. Maintenance of their identity faces major challenges when transitioning through mitosis, as most DNA-binding proteins a ...

EPFL2023

Engineering Materials for Neurotechnology

Diego Ghezzi

Neurotechnology applies methods and devices to mitigate the burden of neurological and mental disorders. In particular, neural interfaces establish a long-term, seamless, symbiotic integration between implants and neural tissue. Materials play a pivotal ro ...

WILEY-V C H VERLAG GMBH2023

Countering T cell exhaustion and senescence for enhanced adoptive T cell therapies against solid tumors

Yang Zhao

CAR-T cell therapy has shown remarkable success in treating hematopoietic malignancies, but its efficacy in solid tumors is limited by T cell dysfunction, including exhaustion and senescence. In this study, we designed metabolically armored CAR-T cells to ...

EPFL2023