Publication

Characterization of flat-fielding systems for quantitative microscopy

Suliana Manley, Dora Mahecic, Khalid Ashraf Mohie Ibrahim
2020
Journal Articles

Abstract

Optical flat-fielding systems, such as field-mapping or integration-based beam shapers, are used to transform nonuniform illumination into uniform illumination. Thus, flat-fielding paves the way for imaging that is independent of position within a field of view and enables more quantitative analysis. Here, we characterize and compare three systems for homogenizing both widefield and multitbcal illumination. Our analysis includes two refractive field-mapping beam shapers: PiShaper and TopShape, as well as one integration-based: Kohler integrator. The comparison is based on figures of merit including ISO-standard values, such as the plateau uniformity and edge steepness, transmission efficiency, stability of the beams along propagation and multifocal intensity, pitch, and point width. By characterizing and comparing existing beam shapers, we facilitate the choice of the appropriate flat-fielding solution and increase their accessibility for different applications. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Official source

https://infoscience.epfl.ch/entities/publication/13333528-cdb4-4581-aea1-38a7d51bdb4e

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Suliana Manley, Dora Mahecic, Khalid Ashraf Mohie Ibrahim
2020
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/13333528-cdb4-4581-aea1-38a7d51bdb4e

About this result

Related concepts (25)

Field (mathematics)

In mathematics, a field is a set on which addition, subtraction, multiplication, and division are defined and behave as the corresponding operations on rational and real numbers do. A field is thus a fundamental algebraic structure which is widely used in algebra, number theory, and many other areas of mathematics. The best known fields are the field of rational numbers, the field of real numbers and the field of complex numbers.

Magnetic field

A magnetic field is a vector field that describes the magnetic influence on moving electric charges, electric currents, and magnetic materials. A moving charge in a magnetic field experiences a force perpendicular to its own velocity and to the magnetic field. A permanent magnet's magnetic field pulls on ferromagnetic materials such as iron, and attracts or repels other magnets.

Quadratic field

In algebraic number theory, a quadratic field is an algebraic number field of degree two over , the rational numbers. Every such quadratic field is some where is a (uniquely defined) square-free integer different from and . If , the corresponding quadratic field is called a real quadratic field, and, if , it is called an imaginary quadratic field or a complex quadratic field, corresponding to whether or not it is a subfield of the field of the real numbers.

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