Publication

Digital holographic microscopy for study cellular dynamics

Christian Depeursinge, Pierre Magistretti, Etienne Cuche, Benjamin Rappaz, Pierre Marquet
2005
Conference Papers

Abstract

Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate noninvasively cellular structures and dynamics. DHM images of living cells in culture are presented. They represent the distribution of the optical path length over the cell, which contains information concerning both the cellular morphometry and the intracellular refractive index, and which has been measured with a sub-wavelength accuracy.

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https://infoscience.epfl.ch/entities/publication/38db94ff-c50e-4076-9e25-f12c230b204a

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Christian Depeursinge, Pierre Magistretti, Etienne Cuche, Benjamin Rappaz, Pierre Marquet
2005
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/38db94ff-c50e-4076-9e25-f12c230b204a

About this result

Related concepts (11)

Microscopy

Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image.

Optical microscope

The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope.

Holography

Holography is a technique that enables a wavefront to be recorded and later re-constructed. Holography is best known as a method of generating real , but it also has a wide range of other applications. In principle, it is possible to make a hologram for any type of wave. A hologram is made by superimposing a second wavefront (normally called the reference beam) on the wavefront of interest, thereby generating an interference pattern which is recorded on a physical medium.

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