Publication

Harmonic holography for three-dimensional cellular imaging

Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh
2009
Conference Papers

Abstract

Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and a broad flexibility in the choice of excitation wavelength. We use the coherent SHG signal from BaTiO3 nanoparticles for three-dimensional (3D) imaging without scanning. We built a harmonic holographic (H2) microscope which records digital holograms at the second harmonic frequency. Highresolution 3D distributions of these SHG markers in mammalian cells are successfully captured and interpreted by the H2 microscope. © 2009 SPIE.

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https://infoscience.epfl.ch/entities/publication/9d105b7b-0c20-4e61-8828-01e3f4d6853a

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Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh
2009
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/9d105b7b-0c20-4e61-8828-01e3f4d6853a

About this result

Related concepts (22)

Second-harmonic generation

Second-harmonic generation (SHG, also called frequency doubling) is a nonlinear optical process in which two photons with the same frequency interact with a nonlinear material, are "combined", and generate a new photon with twice the energy of the initial photons (equivalently, twice the frequency and half the wavelength), that conserves the coherence of the excitation. It is a special case of sum-frequency generation (2 photons), and more generally of harmonic generation.

Nonlinear optics

Nonlinear optics (NLO) is the branch of optics that describes the behaviour of light in nonlinear media, that is, media in which the polarization density P responds non-linearly to the electric field E of the light. The non-linearity is typically observed only at very high light intensities (when the electric field of the light is >108 V/m and thus comparable to the atomic electric field of ~1011 V/m) such as those provided by lasers. Above the Schwinger limit, the vacuum itself is expected to become nonlinear.

High harmonic generation

High harmonic generation (HHG) is a non-linear process during which a target (gas, plasma, solid or liquid sample) is illuminated by an intense laser pulse. Under such conditions, the sample will emit the high harmonics of the generation beam (above the fifth harmonic). Due to the coherent nature of the process, high harmonics generation is a prerequisite of attosecond physics. Perturbative harmonic generation is a process whereby laser light of frequency ω and photon energy ħω can be used to generate new frequencies of light.

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