Publication

Bioanalytical procedure to detect membrane receptor activation

Horst Vogel, Horst Pick
2010
Patents

Abstract

The invention concerns a method for screening compound interaction with transmembrane receptors involved in cellular signal transduction, preferably with G protein coupled receptors, by adding the compound to be analyzed into a medium comprising native inverted vesicles with at least one said transmembrane receptor whose native intracellular side is located on the outer surface of the inverted vesicle.

Official source

https://infoscience.epfl.ch/entities/publication/b8338d3e-9f87-4c48-a843-7c16d4545d24

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Horst Vogel, Horst Pick
2010
Patents

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/b8338d3e-9f87-4c48-a843-7c16d4545d24

About this result

Related concepts (17)

G protein-coupled receptor

G protein-coupled receptors (GPCRs), also known as seven-(pass)-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptors, and G protein-linked receptors (GPLR), form a large group of evolutionarily related proteins that are cell surface receptors that detect molecules outside the cell and activate cellular responses. They are coupled with G proteins.

Cell surface receptor

Cell surface receptors (membrane receptors, transmembrane receptors) are receptors that are embedded in the plasma membrane of cells. They act in cell signaling by receiving (binding to) extracellular molecules. They are specialized integral membrane proteins that allow communication between the cell and the extracellular space. The extracellular molecules may be hormones, neurotransmitters, cytokines, growth factors, cell adhesion molecules, or nutrients; they react with the receptor to induce changes in the metabolism and activity of a cell.

Signal transduction

Signal transduction is the process by which a chemical or physical signal is transmitted through a cell as a series of molecular events. Most commonly, protein phosphorylation is catalyzed by protein kinases, ultimately resulting in a cellular response. Proteins responsible for detecting stimuli are generally termed receptors, although in some cases the term sensor is used. The changes elicited by ligand binding (or signal sensing) in a receptor give rise to a biochemical cascade, which is a chain of biochemical events known as a signaling pathway.

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From Sequence to Dynamics to Function: Computational Design of Allostery and Ligand Selectivity in G-Protein Coupled Receptors

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Structural heterogeneity of the ion and lipid channel TMEM16F

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