Publications related to A high-content image-based drug screen of clinical compounds against cell transmission of adenovirus

Comparison of Three Viral Nucleic Acid Preamplification Pipelines for Sewage Viral Metagenomics

Viral metagenomics is a useful tool for detecting multiple human viruses in urban sewage. However, more refined protocols are required for its effective use in disease surveillance. In this study, we investigated the performance of three different preampli ...

2024

Inactivation of influenza A virus in expiratory droplets and aerosol particles and the associated physicochemical drivers

Aline Laetitia Schaub

Influenza is an infectious respiratory illness caused by influenza viruses. Every year, it causes up to one billion cases of disease worldwide. Despite its high disease burden, the transmission pathway of influenza remains subject to debate. There is incre ...

EPFL2024

Stimulus-responsive assembly of nonviral nucleocapsids

Angela Steinauer

Controlled assembly of a protein shell around a viral genome is a key step in the life cycle of many viruses. Here we report a strategy for regulating the co-assembly of nonviral proteins and nucleic acids into highly ordered nucleocapsids in vitro. By fus ...

Nature Portfolio2024

Peptide-based virucidal drugs

Francesca Olgiati

Before the COVID-19 pandemic, viruses were not a major priority for the scientific community. Today, many events have changed the world and the importance of studying viruses, vaccines, and antiviral drugs is fully appreciated. The development of effective ...

EPFL2024

Stability of influenza A virus in droplets and aerosols is heightened by the presence of commensal respiratory bacteria

Tamar Kohn, Htet Kyi Wynn, Céline Terrettaz, Aline Laetitia Schaub, Athanasios Nenes, Kalliopi Violaki, Marta Augugliaro, Shannon Christa David, Ghislain Gilles Jean-Michel Motos, Walter Hugentobler, Laura José Costa Henriques, Daniel Scott Nolan

Aerosol transmission remains a major challenge for control of respiratory viruses, particularly those causing recurrent epidemics, like influenza A virus (IAV). These viruses are rarely expelled alone, but instead are embedded in a consortium of microorgan ...

American Society for Microbiology2024

Influence of Amino Acid Substitutions in Capsid Proteins of Coxsackievirus B5 on Free Chlorine and Thermal Inactivation

Tamar Kohn, Shotaro Torii, Aleksandar Antanasijevic, Kiruthika Kumar

The sensitivity of enteroviruses to disinfectants varies among genetically similar variants and coincides with amino acid changes in capsid proteins, although the effect of individual substitutions remains unknown. Here, we employed reverse genetics to inv ...

2024

Ligand concentration determines antiviral efficacy of silica multivalent nanoparticles

Francesco Stellacci, Quy Ong Khac, Paulo Henrique Jacob Silva, Heyun Wang, Xufeng Xu, Rémi Andréa La Polla

We have learned from the recent COVID-19 pandemic that the emergence of a new virus can quickly become a global health burden and kill millions of lives. Antiviral drugs are essential in our fight against viral diseases, but most of them are virus-specific ...

Academic Press Inc Elsevier Science2023

Multivalent Virucidal Antivirals: Synthesis, Mechanisms of Action, and Immune-stimulating Effects

Yong Zhu

Viruses initiate invasion by binding to cell surface glycoproteins. Materials mimicking the carbohydrate motifs of these glycoproteins, such as heparan sulfate (HS) and sialic acid (SA) can block viral attachment and inhibit the infection. Multivalent disp ...

EPFL2023

Viral Genetic Determinants of Prolonged Respiratory Syncytial Virus Infection Among Infants in a Healthy Term Birth Cohort

Thomas Junier, Jacques Fellay, Dylan Lawless, Zhi Ming Xu

Background. Respiratory syncytial virus (RSV) is associated with acute respiratory infection. We sought to identify RSV variants associated with prolonged infection. ...

OXFORD UNIV PRESS INC2023

Inactivation mechanisms of influenza A virus under pH conditions encountered in aerosol particles as revealed by whole-virus HDX-MS

Tamar Kohn, Jonathan Paz Montoya, Aline Laetitia Schaub, Athanasios Nenes, Giovanni D'Angelo, Kalliopi Violaki, Shannon Christa David, Ghislain Gilles Jean-Michel Motos, Walter Hugentobler

Multiple respiratory viruses, including influenza A virus (IAV), can be transmitted via expiratory aerosol particles, and aerosol pH was recently identified as a major factor influencing airborne virus infectivity. Indoors, small exhaled aerosols undergo r ...

2023

Simultaneous membrane and RNA binding by Tick-Borne Encephalitis Virus capsid protein

Sarah Victoria Barrass

Tick-borne encephalitis virus is an enveloped, pathogenic, RNA virus in the family Flaviviridae, genus Flavivirus. Viral particles are formed when the nucleocapsid, consisting of an RNA genome and multiple copies of the capsid protein, buds through the end ...

PUBLIC LIBRARY SCIENCE2023

Susceptibility of enveloped and non-enveloped viruses to ultraviolet light-emitting diode (UV-LED) irradiation and implications for virus inactivation mechanisms

Shotaro Torii

Ultraviolet (UV) light-emitting diodes (UV-LEDs), a disinfection technology, efficiently inactivate pathogens in water. However, the assessment of UV-LED treatment and the mechanisms of UV inactivation on viruses (especially enveloped viruses) are limited. ...

ROYAL SOC CHEMISTRY2023

Expiratory Aerosol pH is a Driver of the Persistence of Airborne Influenza A Virus

Aline Laetitia Schaub

To mitigate the spread of a viral disease, it is crucial to understand the factors that influence airborne virus transmission. However, the micro-environment to which the virus is exposed in expiratory aerosol particles is highly complex. The relative humi ...

SWISS CHEMICAL SOC2023

PSGL-1 is an evolutionarily conserved antiviral restriction factor

Chao Jiang, Ying Liu

The arms race between viruses and their hosts shaped the evolutionary history and the genome composition of both parties. Restriction factors are the first-line antiviral effectors encoded by the host genomes and are often conserved through evolution to pr ...

AMER SOC MICROBIOLOGY2023

Antiviral Mechanism of Virucidal Sialic Acid Modified Cyclodextrin

Francesco Stellacci, Paulo Henrique Jacob Silva, Marine Batista, Yong Zhu, Andrey Sysoev

We have reported that CD-6'SLN [6-sialyllactosamine (6'SLN)-modified beta-cyclodextrin (CD)] can be a potential anti-influenza drug because it irreversibly deactivates virions. Indeed, in vivo, CD-6'SLN improved mice survival in an H1N1 infection model eve ...

MDPI2023

CSF-contacting neurons respond to Streptococcus pneumoniae and promote host survival during central nervous system infection

Laura Desban, Ludovic Pierre Gustave Keiser

The pathogenic bacterium Streptococcus pneumoniae (S. pneumoniae) can invade the cerebrospinal fluid (CSF) and cause meningitis with devastating consequences. Whether and how sensory cells in the central nervous system (CNS) become activated during bacteri ...

CELL PRESS2023

Practical considerations of diffusion-weighted MRS with ultra-strong diffusion gradients

André Döring

IntroductionDiffusion-weighted magnetic resonance spectroscopy (DW-MRS) offers improved cellular specificity to microstructure-compared to water-based methods alone-but spatial resolution and SNR is severely reduced and slow-diffusing metabolites necessita ...

Frontiers Media Sa2023

Polyanionic Amphiphilic Dendritic Polyglycerols as Broad-Spectrum Viral Inhibitors with a Virucidal Mechanism

Francesco Stellacci, Valeria Cagno, Matteo Gasbarri

Heparin has been known to be a broad-spectrum inhibitor of viral infection for almost 70 years, and it has been used as a medication for almost 90 years due to its anticoagulant effect. This nontoxic biocompatible polymer efficiently binds to many types of ...

American Chemical Society2022

High-content, arrayed compound screens with rhinovirus, influenza A virus and herpes simplex virus infections

Gerardo Turcatti, Fabien Kuttler

Viruses are genetically and structurally diverse, and outnumber cells by orders of magnitude. They can cause acute and chronic infections, suppress, or exacerbate immunity, or dysregulate survival and growth of cells. To identify chemical agents with pro- ...

2022

Phylogenetic estimation of the viral fitness landscape of HIV-1 set-point viral load

Jacques Fellay, Luca Ferretti

Set-point viral load (SPVL), a common measure of human immunodeficiency virus (HIV)-1 virulence, is partially determined by viral genotype. Epidemiological evidence suggests that this viral property has been under stabilising selection, with a typical opti ...

OXFORD UNIV PRESS2022