Publication

Early Cell Death Detection with Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Pierre Marquet, Nicolas Pavillon, Pascal Jourdain, Corinne Moratal
2012
Journal Articles

Abstract

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it relates to ionic homeostasis, is crucially involved in the activation/inactivation of the cell death processes. We thus present here an application of digital holographic microscopy (DHM) dedicated to early and label-free detection of cell death.

Official source

https://infoscience.epfl.ch/entities/publication/c09c85f8-0133-417c-844c-5cb72f631ea6

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Pierre Magistretti, Christian Depeursinge, Pierre Marquet, Nicolas Pavillon, Pascal Jourdain, Corinne Moratal
2012
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/c09c85f8-0133-417c-844c-5cb72f631ea6

About this result

Related concepts (16)

Quantitative phase-contrast microscopy

FORCETOC Quantitative phase contrast microscopy or quantitative phase imaging are the collective names for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object. Translucent objects, like a living human cell, absorb and scatter small amounts of light. This makes translucent objects much easier to observe in ordinary light microscopes. Such objects do, however, induce a phase shift that can be observed using a phase contrast microscope.

Holography

Holography is a technique that enables a wavefront to be recorded and later re-constructed. Holography is best known as a method of generating real , but it also has a wide range of other applications. In principle, it is possible to make a hologram for any type of wave. A hologram is made by superimposing a second wavefront (normally called the reference beam) on the wavefront of interest, thereby generating an interference pattern which is recorded on a physical medium.

Cell death

Cell death is the event of a biological cell ceasing to carry out its functions. This may be the result of the natural process of old cells dying and being replaced by new ones, as in programmed cell death, or may result from factors such as diseases, localized injury, or the death of the organism of which the cells are part. Apoptosis or Type I cell-death, and autophagy or Type II cell-death are both forms of programmed cell death, while necrosis is a non-physiological process that occurs as a result of infection or injury.

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