Publications related to OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls

Resolving bacterial cell biology: from replisome dynamics to cell wall synthesis

Bacteria are ubiquitous single cellular organisms. Compared to eukaryotic cells, bacteria have two unique characteristics: the membrane-less nucleoid and the cell wall built of peptidoglycan (PG). In most bacteria, a single circular chromosome is compacted ...

EPFL2024

Quantification of Native Lignin Structural Features with Gel-Phase 2D-HSQC0 Reveals Lignin Structural Changes During Extraction

Jeremy Luterbacher, Stefania Bertella

Our ability to study and valorize the lignin fraction of biomass is hampered by the fundamental and still unmet challenge of precisely quantifying native lignin's structural features. Here, we developed a rapid elevated-temperature H-1-C-13 Heteronuclear S ...

Wiley-VCH Verlag GmbH2024

Quantified interface chemistry and biophysics via water imaging

Sylvie Roke

High throughput wide-field second harmonic imaging enables the label-free imaging of interfacial (< 3 nm thick) water, with a spatial resolution of similar to 370 nm using similar to 100 ms acquisition times per image. The obtained interfacial orientationa ...

Spie-Int Soc Optical Engineering2024

Synthesis and application of bifunctional hypervalent iodine reagents for peptide modification

Elija Grinhagena

Protein-protein interactions (PPIs) are responsible for regulating many biological processes in human bodies. PPIs often have a large binding site and often interact through an alpha helical segment. Therefore, the inhibition of PPIs can be difficult using ...

EPFL2023

Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes

Marcos Penedo Garcia

Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures i ...

2023

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

Samuel Mendes Leitão

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

EPFL2023

Phloem water isotopically different to xylem water: Potential causes and implications for ecohydrological tracing

Andrea Rinaldo, Paolo Benettin

The stable isotopes of hydrogen and oxygen in xylem water are often used to investigate tree water sources. But this traditional approach does not acknowledge the contribution of water stored in the phloem to transpiration and how this may affect xylem wat ...

WILEY2022

Carbon nanotube uptake in cyanobacteria for near-infrared imaging and enhanced bioelectricity generation in living photovoltaics

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Alessandra Antonucci, Charlotte Elisabeth Marie Roullier, Nils Schürgers, Benjamin Paul Johanès Gabriel Lambert, Alice Judith Gillen, Melania Reggente, Mohammed Mouhib

Single-walled carbon nanotubes (SWCNTs) have unique optoelectronic properties that make them suitable for applications ranging from phototherapy to imaging and sensing, but their uptake has mainly been explored in eukaryotic cells. Here the authors explore ...

2022

Fluorescent D-Amino Acids for Super-resolution Microscopy of the Bacterial Cell Wall

Luc Reymond, Suliana Manley, Ophélie Rutschmann, Juliette Griffie, Chen Zhang, Willi Leopold Stepp, Jiangtao Qiao, Faustine Ambroisine Charline Ryckebusch

Fluorescent D-amino acids (FDAAs) have previ-ously been developed to enable in situ highlighting of locations of bacterial cell wall growth. Most bacterial cells lie at the edge of the diffraction limit of visible light; thus, resolving the precise details ...

2022

Multivalent Peptide Ligands To Probe the Chromocenter Microenvironment in Living Cells

Nora Guidotti, Maxime Nicolas Mivelaz, Beat Fierz, Pablo Rivera Fuentes, Adam Miklos Eördögh

Chromatin is spatially organized into functional states that are defined by both the presence of specific histone post-translational modifications (PTMs) and a defined set of chromatin-associated “reader” proteins. Different models for the underlying mecha ...

2022

An optimized high throughput platform, from genetic transformation to fermentation, to screen for secreted laccases in Aspergillus niger

Laure Marie Colette Leynaud-Kieffer Curran

Many challenges are faced in the conversion of biomass into advanced biofuels, one of which is finding the correct organism for the job. The filamentous fungus Aspergillus niger has been chosen as a biocatalyst for cellulose, hemicellulose, and lignin degr ...

EPFL2021

Preparation and Analysis of Bacteria Surface-Modified with Polymer Nanoparticles

Lisa Patricia Elisabeth Bader

Cell-based drug carriers present an interesting approach to improve the targeted delivery of therapeutic drugs in vivo. Specifically, bacterial cells possess interesting properties such as motility and sensing that allow some strains to selectively target ...

EPFL2022

RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment Reveals Upregulation in Plant Stress and Defense Pathways

Alan Howling, Ivo Furno, Alexandra Waskow

Not all agricultural practices are sustainable; however, non-thermal plasma treatment of seeds may be an eco-friendly alternative to improve macroscopic plant growth parameters. Despite the numerous successful results of plasma-seed treatments reported in ...

2022

Benthic Biofilms in Glacier-Fed Streams from Scandinavia to the Himalayas Host Distinct Bacterial Communities Compared with the Streamwater

Leïla Ezzat, Vincent Henri De Staercke, Tom Ian Battin, Paraskevi Pramateftaki, Hannes Markus Peter, Emmy Marie Oppliger, Jade Brandani, Matteo Tolosano, Michail Styllas, Tyler Joe Kohler, Stylianos Fodelianakis, Massimo Bourquin, Martina Andrea Schön, Susheel Bhanu Busi, Grégoire Marie Octave Edouard Michoud, Virginia Tadei

Microbial life in glacier-fed streams (GFSs) is dominated by benthic biofilms which fulfill critical ecosystem processes. However, it remains unclear how the bacterial communities of these biofilms assemble in stream ecosystems characterized by rapid turno ...

AMER SOC MICROBIOLOGY2022

Extraction and controlled functionalization of lignin for materials applications

Stefania Bertella

Lignin is a renewable aromatic polymer that due to its abundance and unique chemical structure is a promising candidate to replace aromatic materials that are currently sourced from fossil oil. The same structure of lignin poses however drawbacks for its v ...

EPFL2022

Postnatal expansion of mesenteric lymph node stromal cells towards reticular and CD34(+) stromal cell subsets

Bart Deplancke, Julie Marie Russeil, Wanze Chen, Vincent Roland Julien Gardeux, Maria Litovchenko, Marjan Biocanin, Jörn Pezoldt, Guido Van Mierlo

Gut-draining mesenteric lymph nodes (LN) provide the framework to shape intestinal adaptive immune responses. Based on the transcriptional signatures established by our previous work, the composition and immunomodulatory function of LN stromal cells (SC) v ...

Nature Portfolio2022

Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM

Marcos Penedo Garcia

Atomic force microscopy (AFM) is the only technique that allows label-free imaging of nanoscale biomolecular dynamics, playing a crucial role in solving biological questions that cannot be addressed by other major bioimaging tools (fluorescence or electron ...

AMER ASSOC ADVANCEMENT SCIENCE2021

Quantum Sensing of Free Radicals in Primary Human Dendritic Cells

Mayeul Sylvain Chipaux

Free radicals are crucial indicators for stress and appear in all kinds of pathogenic conditions, including cancer, cardiovascular diseases, and infection. However, they are difficult to detect due to their reactivity and low abundance. We use relaxometry ...

AMER CHEMICAL SOC2022

Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

Marcos Penedo Garcia

Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in par ...

Nature Research2021

New and improved methods for integrated models of metabolism and gene expression

Pierre Guy Rémy Salvy

The beginning of the 21st century was marked by the advent of disruptive technologies, which ushered an era of groundbreaking advances in fundamental sciences, carried by the great pace at which computational capabilities spread and evolved. But the new ce ...

EPFL2021