Publications related to Accelerated autofocusing of off-axis holograms using critical sampling

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Claudio Bruschini, Edoardo Charbon, Arin Can Ülkü, Yichen Feng

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

SPIE Society of Photo-optical Instrumentation Engineers2024

In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera

Claudio Bruschini, Edoardo Charbon, Arin Can Ülkü

Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche diode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM ...

Optica Publishing Group2022

Single molecule fluorescence and phosphorescence studies using a scanning tunneling microscope

Abhishek Grewal

This thesis investigates novel single-molecule luminescence phenomena at their inherent, sub-molecular length scale. The microscopic understanding of luminescence processes will be crucial for the continued improvement of organic optoelectronic and semicon ...

EPFL2022

On the Grothendieck-Serre conjecture for classical groups

Eva Bayer Fluckiger

We prove some new cases of the Grothendieck-Serre conjecture for classical groups. This is based on a new construction of the Gersten-Witt complex for Witt groups of Azumaya algebras with involution on regular semilocal rings, with explicit second residue ...

WILEY2022

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Michael Stefan Daniel Liebling, Christian Jaques

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

2020

Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium

Claudio Bruschini, Edoardo Charbon, Arin Can Ülkü

Fluorescence lifetime imaging (FLI) is increasingly recognized as a powerful tool for biochemical and cellular investigations, including in vivo applications. Fluorescence lifetime is an intrinsic characteristic of any fluorescent dye which, to a large ext ...

AMER CHEMICAL SOC2020

Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells

Karl Gademann, Simon Sieber, Leo Scheller

We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian c ...

2020

Aliasing mitigation in optical microscopy of dynamic biological samples by use of temporally modulated color illumination and a standard RGB camera

Michael Stefan Daniel Liebling, Christian Jaques

Significance: Despite recent developments in microscopy, temporal aliasing can arise when imaging dynamic samples. Modern sampling frameworks, such as generalized sampling, mitigate aliasing but require measurement of temporally overlapping and potentially ...

SPIE Society of Photo-optical Instrumentation Engineers2020

Beyond Cameras: Real-Time High-Resolution 3D and Panoramic MultiView Vision Systems with Their Applications

Bilal Demir

The cameras are invented by imitating the human visual system to capture the scene. The camera technologies have been substantially advanced in recent years. 108 MP resolution with 100x hybrid zoom has become standard features for smartphone flagships. In ...

EPFL2020

Waveguide platform and methods for super-resolution fluorescence microscopy of sub-cellular structures

Anna Archetti

Super-resolution fluorescence microscopy is widespread, owing to its demonstrated ability to resolve dynamical processes within cells and to identify the structure and position of specific proteins in the interior of protein complexes. Nowadays, subcellula ...

EPFL2019

Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Alessandra Antonucci, Benjamin Paul Johanès Gabriel Lambert

Fluorescence microscopy in the second near-infrared optical window (NIR-II, 1000–1350 nm) has become a technique of choice for non-invasive in vivo imaging. The deep penetration of NIR light in living tissue, as well as negligible tissue autofluorescence w ...

Nature Research2018

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

The multi-spectral imaging diagnostic

Basil Duval, Yanis Andrebe, Christian Gabriel Theiler, Kevin Henricus Annemarie Verhaegh, Mirko Wensing

The Multi-Spectral Imaging system is a new diagnostic that captures simultaneous spectrally filtered images from a common line of sight while maintaining a large etendue and high throughput. Imaging several atomic line intensities simultaneously may enable ...

2018

Nanopatterning of a Stimuli-Responsive Fluorescent Supramolecular Polymer by Thermal Scanning Probe Lithography

Jürgen Brugger, Samuel Zimmermann

The miniaturization of nanometer-sized multicolor fluorescent features is of continuous significance for counterfeit security features, data storage, and sensors. Recent advances in engineering of stimuli-responsive supramolecular polymeric materials that ...

2017

Intense fluorescence of Au-20

Harald Brune, Wolfgang Harbich, Chongqi Yu

Ligand-protected Au clusters are non-bleaching fluorescence markers in bio- and medical applications. Here we show that their fluorescence can be an intrinsic property of the Au cluster itself. We find a very intense and sharp fluorescence peak located at ...

American Institute of Physics2017

Embeddings of maximal tori in classical groups over local and global fields

Eva Bayer Fluckiger, Ting-Yu Lee

Embeddings of maximal tori in classical groups over fields of characteristic not 2 are the subject matter of several recent papers. The aim of the present paper is to give necessary and sufficient conditions for such an embedding to exist, when the base fi ...

Turpion Ltd2016

Image processing on reconfigurable hardware for continuous monitoring of fluorescent biomarkers in cell cultures

Julien Michel Ghaye

Fluorescence microscopy is a widespread tool in biological research. It is the primary modality for bioimaging and empowers the study and analysis of multitudes of biological processes. It can be applied to fixed biosamples, that is samples with frozen bio ...

EPFL2015

Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix

Hubert van den Bergh, Georges Wagnières, Matthieu Philippe Emmanuel Zellweger, Blaise Lovisa, Carla Martoccia

Fluorescence cystoscopy enhances detection of early bladder cancer. Water used to inflate the bladder during the procedure rapidly contains urine, which may contain fluorochromes. This frequently degrades fluorescence images. Samples of bladder washout flu ...

SPIE Society of Photo-optical Instrumentation Engineers2014

Optical microscopy for imaging through scattering media

Xin Yang

Optical microscopy has been widely used in the life sciences and biological research for several decades. It possesses many advantages over x-ray radiography, computed tomography, ultrasound imaging and magnetic resonance imaging, in terms of being low cos ...

EPFL2014

Functional and Structural Imaging with Optical Coherence Microscopy : Principles & Applications

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013