Publication

Protein-Functionalized Polymer Brushes

Harm-Anton Klok, Kai Johnsson, Stefano Tugulu, India Christina Sielaff, Anke Arnold
2005
Journal Articles

Abstract

A new strategy for the prepn. of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymn. (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O6-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O6-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface d. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/d0b6cebb-fb8d-4ab8-9a16-8ad950fe93a9

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Harm-Anton Klok, Kai Johnsson, Stefano Tugulu, India Christina Sielaff, Anke Arnold
2005
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/d0b6cebb-fb8d-4ab8-9a16-8ad950fe93a9

About this result

Related concepts (9)

Polymer

A polymer (ˈpɒlᵻmər; Greek poly-, "many" + -mer, "part") is a substance or material consisting of very large molecules called macromolecules, composed of many repeating subunits. Due to their broad spectrum of properties, both synthetic and natural polymers play essential and ubiquitous roles in everyday life. Polymers range from familiar synthetic plastics such as polystyrene to natural biopolymers such as DNA and proteins that are fundamental to biological structure and function.

Protein microarray

A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel. The chip consists of a support surface such as a glass slide, nitrocellulose membrane, bead, or microtitre plate, to which an array of capture proteins is bound. Probe molecules, typically labeled with a fluorescent dye, are added to the array.

Hair conditioner

Hair conditioner is a hair care cosmetic product used to improve the feel, texture, appearance and manageability of hair. Its main purpose is to reduce friction between strands of hair to allow smoother brushing or combing, which might otherwise cause damage to the scalp. Various other benefits are often advertised, such as hair repair, strengthening, or a reduction in split ends. Conditioners are available in a wide range of forms, including viscous liquids, gels and creams, as well as thinner lotions and sprays.

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