MicroscopyMicroscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image.
Confocal microscopyConfocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a sample enables the reconstruction of three-dimensional structures (a process known as optical sectioning) within an object.
Super-resolution microscopySuper-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, which is due to the diffraction of light. Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy as well as those that use the Pendry Superlens and near field scanning optical microscopy) or on the far-field.
Transverse waveIn physics, a transverse wave is a wave whose oscillations are perpendicular to the direction of the wave's advance. This is in contrast to a longitudinal wave which travels in the direction of its oscillations. Water waves are an example of transverse wave. A simple example is given by the waves that can be created on a horizontal length of string by anchoring one end and moving the other end up and down. Another example is the waves that are created on the membrane of a drum.
Nonlinear opticsNonlinear optics (NLO) is the branch of optics that describes the behaviour of light in nonlinear media, that is, media in which the polarization density P responds non-linearly to the electric field E of the light. The non-linearity is typically observed only at very high light intensities (when the electric field of the light is >108 V/m and thus comparable to the atomic electric field of ~1011 V/m) such as those provided by lasers. Above the Schwinger limit, the vacuum itself is expected to become nonlinear.
CarA car, or an automobile is a motor vehicle with wheels. Most definitions of cars say that they run primarily on roads, seat one to eight people, have four wheels, and mainly transport people, not cargo. French inventor Nicolas-Joseph Cugnot built the first steam-powered road vehicle in 1769, while French-born-Swiss inventor François Isaac de Rivaz designed and constructed the first internal combustion powered automobile in 1808. The modern car—a practical, marketable automobile for everyday use—was invented in 1886, when German inventor Carl Benz patented his Benz Patent-Motorwagen.
Sine waveA sine wave, sinusoidal wave, or sinusoid is a mathematical curve defined in terms of the sine trigonometric function, of which it is the graph. It is a type of continuous wave and also a smooth periodic function. It occurs often in mathematics, as well as in physics, engineering, signal processing and many other fields. Its most basic form as a function of time (t) is: where: A, amplitude, the peak deviation of the function from zero. f, ordinary frequency, the number of oscillations (cycles) that occur each second of time.
Square waveA square wave is a non-sinusoidal periodic waveform in which the amplitude alternates at a steady frequency between fixed minimum and maximum values, with the same duration at minimum and maximum. In an ideal square wave, the transitions between minimum and maximum are instantaneous. The square wave is a special case of a pulse wave which allows arbitrary durations at minimum and maximum amplitudes. The ratio of the high period to the total period of a pulse wave is called the duty cycle.
ImageAn image is a visual representation of something. An image can be a two-dimensional (2D) representation, such as a drawing, painting, or photograph, or a three-dimensional (3D) object, such as a carving or sculpture. An image may be displayed through other media, including projection on a surface, activation of electronic signals, or digital displays. Two-dimensional images can be still or animated. Still images can usually be reproduced through mechanical means, such as photography, printmaking or photocopying.
Network layerIn the seven-layer OSI model of computer networking, the network layer is layer 3. The network layer is responsible for packet forwarding including routing through intermediate routers. The network layer provides the means of transferring variable-length network packets from a source to a destination host via one or more networks. Within the service layering semantics of the OSI (Open Systems Interconnection) network architecture, the network layer responds to service requests from the transport layer and issues service requests to the data link layer.
Link layerIn computer networking, the link layer is the lowest layer in the Internet protocol suite, the networking architecture of the Internet. The link layer is the group of methods and communications protocols confined to the link that a host is physically connected to. The link is the physical and logical network component used to interconnect hosts or nodes in the network and a link protocol is a suite of methods and standards that operate only between adjacent network nodes of a network segment.
Session layerIn the seven-layer OSI model of computer networking, the session layer is layer 5. The session layer provides the mechanism for opening, closing and managing a session between end-user application processes, i.e., a semi-permanent dialogue. Communication sessions consist of requests and responses that occur between applications. Session-layer services are commonly used in application environments that make use of remote procedure calls (RPCs). An example of a session-layer protocol is the OSI protocol suite session-layer protocol, also known as X.
French New WaveThe New Wave (Nouvelle Vague), also called the French New Wave, is a French art film movement that emerged in the late 1950s. The movement was characterized by its rejection of traditional filmmaking conventions in favor of experimentation and a spirit of iconoclasm. New Wave filmmakers explored new approaches to editing, visual style, and narrative, as well as engagement with the social and political upheavals of the era, often making use of irony or exploring existential themes.
Boundary layerIn physics and fluid mechanics, a boundary layer is the thin layer of fluid in the immediate vicinity of a bounding surface formed by the fluid flowing along the surface. The fluid's interaction with the wall induces a no-slip boundary condition (zero velocity at the wall). The flow velocity then monotonically increases above the surface until it returns to the bulk flow velocity. The thin layer consisting of fluid whose velocity has not yet returned to the bulk flow velocity is called the velocity boundary layer.
Central AmericaCentral America is a subregion of the Americas. Its political boundaries are defined as bordering Mexico to the north, Colombia to the south, the Caribbean Sea to the east, and the Pacific Ocean to the west. Central America usually consists of seven countries: Belize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, and Panama. Within Central America is the Mesoamerican biodiversity hotspot, which extends from northern Guatemala to central Panama.
Presentation layerIn the seven-layer OSI model of computer networking, the presentation layer is layer 6 and serves as the data translator for the network. It is sometimes called the syntax layer. Within the service layering semantics of the OSI network architecture, the presentation layer responds to service requests from the application layer and issues service requests to the session layer through a unique presentation service access point (PSAP).
Transport layerIn computer networking, the transport layer is a conceptual division of methods in the layered architecture of protocols in the network stack in the Internet protocol suite and the OSI model. The protocols of this layer provide end-to-end communication services for applications. It provides services such as connection-oriented communication, reliability, flow control, and multiplexing. The details of implementation and semantics of the transport layer of the Internet protocol suite, which is the foundation of the Internet, and the OSI model of general networking are different.
Two-photon excitation microscopyTwo-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness. Unlike traditional fluorescence microscopy, where the excitation wavelength is shorter than the emission wavelength, two-photon excitation requires simultaneous excitation by two photons with longer wavelength than the emitted light. The laser is focused onto a specific location in the tissue and scanned across the sample to sequentially produce the image.
ImagingImaging is the representation or reproduction of an object's form; especially a visual representation (i.e., the formation of an ). Imaging technology is the application of materials and methods to create, preserve, or duplicate images. Imaging science is a multidisciplinary field concerned with the generation, collection, duplication, analysis, modification, and visualization of images, including imaging things that the human eye cannot detect.
North AmericaNorth America is a continent in the Northern Hemisphere almost entirely within the Western Hemisphere. North America is bordered to the north by the Arctic Ocean, to the east by the Atlantic Ocean, to the southeast by South America and the Caribbean Sea, and to the west and south by the Pacific Ocean. The region includes Canada, the Caribbean, Central America, Greenland, Mexico, and the United States. North America covers an area of about , representing approximately 16.5% of the Earth's land area and 4.
