Publication

Microfluidic Single-Cell Analysis with Affinity Beads

Horst Vogel, Rudolf Hovius, Michael Werner, Loïc Arvind Roger Arm
2015
Journal Articles

Abstract

A micrometer-sized affinity bead (red) is (i) taken up into a cell by phagocytosis, (ii) photochemically released from phagosomes, (iii) optically trapped by the cell, and (iv) isolated by cell lysis for subsequent analysis of captured intracellular analyte (green). © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Official source

https://infoscience.epfl.ch/entities/publication/e98f41b3-f978-4c1a-8d2d-173f3acce9c1

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Horst Vogel, Rudolf Hovius, Michael Werner, Loïc Arvind Roger Arm
2015
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/e98f41b3-f978-4c1a-8d2d-173f3acce9c1

About this result

Related concepts (12)

Droplet-based microfluidics

Droplet-based microfluidics manipulate discrete volumes of fluids in immiscible phases with low Reynolds number and laminar flow regimes. Interest in droplet-based microfluidics systems has been growing substantially in past decades. Microdroplets offer the feasibility of handling miniature volumes (μl to fl) of fluids conveniently, provide better mixing, encapsulation, sorting, sensing and are suitable for high throughput experiments.

Magneto-optical trap

In condensed matter physics, a magneto-optical trap (MOT) is an apparatus which uses laser cooling and a spatially-varying magnetic field to create a trap which can produce samples of cold, neutral atoms. Temperatures achieved in a MOT can be as low as several microkelvin, depending on the atomic species, which is two or three times below the photon recoil limit. However, for atoms with an unresolved hyperfine structure, such as , the temperature achieved in a MOT will be higher than the Doppler cooling limit.

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Optical tweezers (originally called single-beam gradient force trap) are scientific instruments that use a highly focused laser beam to hold and move microscopic and sub-microscopic objects like atoms, nanoparticles and droplets, in a manner similar to tweezers. If the object is held in air or vacuum without additional support, it can be called optical levitation. The laser light provides an attractive or repulsive force (typically on the order of piconewtons), depending on the relative refractive index between particle and surrounding medium.

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