Publication

Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane

Sebastian Maerkl, Jose Luis Garcia Cordero
2013
Journal Articles

Abstract

We report the flow-based in situ patterning of multiple proteins in microfluidic channels by simply tuning the actuation pressure of a microfluidic button-membrane. We show that a single button-membrane can pattern several concentric protein annuli, and we apply this patterning approach to a multiplexed immunoassay.

Official source

https://infoscience.epfl.ch/entities/publication/fd9ced6b-8c65-4d12-91ba-0ec75f2fcfae

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Sebastian Maerkl, Jose Luis Garcia Cordero
2013
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/fd9ced6b-8c65-4d12-91ba-0ec75f2fcfae

About this result

Related concepts (14)

Microfluidics

Microfluidics refers to a system that manipulates a small amount of fluids ((10−9 to 10−18 liters) using small channels with sizes ten to hundreds micrometres. It is a multidisciplinary field that involves molecular analysis, biodefence, molecular biology, and microelectronics. It has practical applications in the design of systems that process low volumes of fluids to achieve multiplexing, automation, and high-throughput screening.

Droplet-based microfluidics

Droplet-based microfluidics manipulate discrete volumes of fluids in immiscible phases with low Reynolds number and laminar flow regimes. Interest in droplet-based microfluidics systems has been growing substantially in past decades. Microdroplets offer the feasibility of handling miniature volumes (μl to fl) of fluids conveniently, provide better mixing, encapsulation, sorting, sensing and are suitable for high throughput experiments.

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Paper-based microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that transport fluid from an inlet through the porous medium to a desired outlet or region of the device, by means of capillary action. This technology builds on the conventional lateral flow test which is capable of detecting many infectious agents and chemical contaminants. The main advantage of this is that it is largely a passively controlled device unlike more complex microfluidic devices.

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