Publications associées à Fate mapping

Unequal cleavage of the C. elegans zygote ensures robust embryogenesis

Asymmetric cell divisions play key roles in generating cellular diversity during embryogenesis, and in tissue homeostasis during postembryonic life. Particular asymmetric cell divisions producing differentially sized daughter cells were described in many s ...

EPFL2020

Probing the distribution of cortical components, including PtdIns(4,5)P2, during asymmetric division of C. elegans embryos

Melina Jasmin Scholze

Asymmetric cell division is crucial for embryonic development and stem cell lineages. In the one-cell C. elegans embryo, a contractile cortical actomyosin network contributes to asymmetric division by segregating PAR proteins to discrete cortical domains. ...

EPFL2018

8-Channel Single Embryo Pipette For Accurate C-Elegans Bioassays

Martinus Gijs, Thomas Lehnert, Li Dong, Jingwei Zhang

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or bioch ...

IEEE2018

Polarity-Dependent Asymmetric Distribution and MEX-5/6-Mediated Translational Activation of the Era-1 mRNA in C. elegans Embryos

Pierre Gönczy

The early C. elegans embryo is an attractive model system to investigate fundamental developmental processes. With the exception of mex-3 mRNA, maternally contributed mRNAs are thought to be distributed uniformly in the one-cell embryo. Here, we report and ...

Public Library of Science2015

Quantitative Analysis and Modeling Probe Polarity Establishment in C. elegans Embryos

Pierre Gönczy, Felix Naef, Coralie Busso, Simon Blanchoud

Cell polarity underlies many aspects of metazoan development and homeostasis, and relies notably on a set of PAR proteins located at the cell cortex. How these proteins interact in space and time remains incompletely understood. We performed a quantitative ...

Cell Press2015

Polarity establishment, asymmetric division and segregation of fate determinants in early C. elegans embryos

Pierre Gönczy

Polarity establishment, asymmetric division, and acquisition of cell fates are critical steps during earlydevelopment. In this review, we discuss processes that set up the embryonic axes, with an emphasis onpolarity establishment and asymmetric division. W ...

WormBook2014

Mutual Inhibition Between the Anaphase-promoting Complex and the Spindle Assembly Checkpoint in C. Elegans Embryos

Alexandra Bezler

The cell cycle orchestrates timely duplication and distribution of cellular components to daughter cells. Checkpoints operate to survey the faithful completion of specific steps during the cell cycle, thus ensuring the equal segregation of genetic material ...

EPFL2011

ASSET: A robust algorithm for the automated segmentation and standardization of early Caenorhabditis elegans embryos

Pierre Gönczy, Felix Naef, Simon Blanchoud, Yemima Suryani Budirahardja

The early Caenorhabditis elegans embryo is an attractive model to investigate evolutionarily conserved cellular mechanisms. However, there is a paucity of automated methods to gather quantitative information with subcellular precision in this system. We de ...

Wiley-Blackwell2010

Temperature gradient stimulation for cell division in C. Elegans Embryos on chip

Philippe Renaud, Pierre Gönczy, Sophie Baranek Delasoie, Alexandra Bezler, Christian Adamczyk

This paper reports on a new microfluidic device for temperature stimulation of cell in in-vitro culture. Micro- electrodes in a meander shape are embedded into the microfluidic channels to generate either a temperature gradient through the culture chamber ...

2010

PLK-1 asymmetry contributes to asynchronous cell division of C. elegans embryos

Pierre Gönczy, Yemima Suryani Budirahardja

Acquisition of lineage-specific cell cycle duration is an important feature of metazoan development. In Caenorhabditis elegans, differences in cell cycle duration are already apparent in two-cell stage embryos, when the larger anterior blastomere AB divide ...

2008

Cortical localization of the Galpha protein GPA-16 requires RIC-8 function during C. elegans asymmetric cell division

Pierre Gönczy

Understanding of the mechanisms governing spindle positioning during asymmetric division remains incomplete. During unequal division of one-cell stage C. elegans embryos, the Galpha proteins GOA-1 and GPA-16 act in a partially redundant manner to generate ...

2005