Publications associées à Organogenèse

Puckered and JNK signaling in pioneer neurons coordinates the motor activity of the Drosophila embryo

Central nervous system organogenesis is a complex process that obeys precise architectural rules. The impact that nervous system architecture may have on its functionality remains, however, relatively unexplored. To clarify this problem, we analyze the dev ...

Nature Portfolio2023

An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates

Matthias Lütolf, Sebastian Maerkl, Hao-An Hsiung, Zoe Newell Swank, Stefano Davide Vianello, Julia Tischler

We present a low-cost, do-it-yourself system for complexmammalian cell culture under dynamically changing medium formulations by integrating conventional multi-well tissue culture plates with simple microfluidic control and system automation. We demonstrat ...

2022

Preparation of Single-Somite Explants from Zebrafish Embryos

Andrew Charles Oates, Sundar Ram Naganathan

The body axis of vertebrate embryos is periodically subdivided into 3D multicellular units called somites. While genetic oscillations and molecular prepatterns determine the initial length-scale of somites, mechanical processes have been implicated in sett ...

JOURNAL OF VISUALIZED EXPERIMENTS2022

Reaction wavefront theory of notochord segment patterning

Andrew Charles Oates

The vertebrate axis is segmented into repetitive structures, the vertebrae. In fish, these segmented structures are thought to form from the paraxial mesoderm and the adjacent notochord. Recent work revealed an autonomous patterning mechanism in the zebraf ...

FRONTIERS MEDIA SA2022

In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture

Stefano Davide Vianello

In humans, mice, and other mammals key internal organs such as the gut, the lungs, the pancreas, and the liver all derive from the same embryonic tissue: the endoderm. The development of all of these structures thus depends on a same set of early cells, an ...

EPFL2022

Differential impact of TGF beta/SMAD signaling activity elicited by Activin A and Nodal on endoderm differentiation of epiblast stem cells

Pierre Osteil

Allocation of cells to an endodermal fate in the gastrulating embryo is driven by Nodal signaling and consequent activation of TGF beta pathway. In vitro methodologies striving to recapitulate the process of endoderm differentiation, however, use TGF beta ...

WILEY2022

Organoids as tools for fundamental discovery and translation-A Keystone Symposia report

Matthias Lütolf

Complex three-dimensional in vitro organ-like models, or organoids, offer a unique biological tool with distinct advantages over two-dimensional cell culture systems, which can be too simplistic, and animal models, which can be too complex and may fail to ...

WILEY2022

Defined matrices for epithelial organoid culture and disease modelling

Saba Rezakhani

Advances in our understanding of the biology of stem cells and their niches have provided the necessary foundation to create in vitro complex self-organizing three-dimensional (3D) structures called organoids. While organoids represent a highly promising n ...

EPFL2021

Towards engineering an artificial neural tube

Jisoo Park

In this thesis, I present a self-organizing neural tube organoid that is strikingly similar in morphology, cell-type composition, and patterning to the mouse embryonic neural tube. When exposed to a sequence of epiblast culture conditions and neural differ ...

EPFL2021

Extracellular matrix requirements for gastrointestinal organoid cultures

Organoids are a new class of biological model systems that have garnered significant interest in the life sciences. When provided with the proper 3D matrix and biochemical factors, stem cells can self-organize and form tissue-specific organoids. Thus far, ...

2021

What are you synching about? Emerging complexity of Notch signaling in the segmentation clock

Andrew Charles Oates, Olivier François Venzin

The Segmentation clock is a population of cellular genetic oscillators, located in the posterior of the elongating vertebrate embryo, that governs the rhythmic and sequential segmentation of the body axis into somites. Somites are blocks of cells that give ...

ACADEMIC PRESS INC ELSEVIER SCIENCE2020

High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays

Matthias Lütolf, Gerardo Turcatti, Nathalie Brandenberg, George Coukos, Fabien Kuttler, Krisztian Homicsko, Camilla Carla Alma Ceroni, Nikolche Gjorevski

Stem-cell-derived epithelial organoids are routinely used for the biological and biomedical modelling of tissues. However, the complexity, lack of standardization and quality control of stem cell culture in solid extracellular matrices hampers the routine ...

NATURE PUBLISHING GROUP2020

Low-Defect Thiol-Michael Addition Hydrogels as Matrigel Substitutes for Epithelial Organoid Derivation

Matthias Lütolf, Nikolche Gjorevski, Saba Rezakhani

Due to their selective crosslinking chemistry and well-defined mechano-biochemical characteristics, hydrogels crosslinked by thiol-Michael addition reactions have garnered immense interest for 3D cell culture applications. These hydrogels are typically for ...

2020

Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids

Denis Duboule, Matthias Lütolf, Leonardo Beccari, Mehmet Ugur Girgin, Anne-Catherine Cossy, Alfonso Martinez Arias

The emergence of multiple axes is an essential element in the establishment of the mammalian body plan. This process takes place shortly after implantation of the embryo within the uterus and relies on the activity of gene regulatory networks that coordina ...

NATURE PUBLISHING GROUP2018

Mutations in Bcl9 and Pygo genes cause congenital heart defects by tissue-specific perturbation of Wnt/beta-catenin signaling

Michel Aguet

Bcl9 and Pygopus (Pygo) are obligate Wnt/beta-catenin cofactors in Drosophila, yet their contribution to Wnt signaling during vertebrate development remains unresolved. Combining zebrafish and mouse genetics, we document a conserved, beta-catenin-associate ...

COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT2018

Essential role of the TFIID subunit TAF4 in murine embryogenesis and embryonic stem cell differentiation

Daniel Alpern

TAF4 (TATA-binding protein-associated factor 4) and its paralogue TAF4b are components of the TFIID core module. We inactivated the murine Taf4a gene to address Taf4 function during embryogenesis. Here we show that Taf4a(-/-) embryos survive until E9.5 whe ...

Nature Publishing Group2016

Faster embryonic segmentation through elevated Delta-Notch signalling

Andrew Charles Oates

An important step in understanding biological rhythms is the control of period. A multicellular, rhythmic patterning system termed the segmentation clock is thought to govern the sequential production of the vertebrate embryo's body segments, the somites. ...

Nature Publishing Group2016

Concise Reviews: In Vitro-Produced Pancreas Organogenesis Models in Three Dimensions: Self-Organization From Few Stem Cells or Progenitors

Filippo De Franceschi, Anne Grapin-Botton, Chiara Greggio

Three-dimensional models of organ biogenesis have recently flourished. They promote a balance between stem/progenitor cell expansion and differentiation without the constraints of flat tissue culture vessels, allowing for autonomous self-organization of ce ...

Wiley-Blackwell2015

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

Filippo De Franceschi, Anne Grapin-Botton, Chiara Greggio

The pancreas is an essential organ that regulates glucose homeostasis and secretes digestive enzymes. Research on pancreas embryogenesis has led to the development of protocols to produce pancreatic cells from stem cells. The whole embryonic organ can be c ...

Journal Of Visualized Experiments2014

3D Reconstitution of the Patterned Neural Tube from Embryonic Stem Cells

Matthias Lütolf, Adrian Ranga

Inducing organogenesis in 3D culture is an important aspect of stem cell research. Anterior neural structures have been produced from large embryonic stem cell (ESC) aggregates, but the steps involved in patterning such complex structures have been ill def ...

Cell Press2014