Publications associées à Coloration Hoechst

Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes

Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures i ...

2023

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Demetri Psaltis, Joowon Lim, Daniele Pirone

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

Nature Publishing Group2022

Block Copolymer Stabilized Liquid Nanodroplets Facilitate Efficient Triplet Fusion-Based Photon Upconversion in Solid Polymer Matrices

Felipe Andres Saenz Padilla

Sensitized triplet-triplet annihilation-based photon upconversion is a photo-physical process that affords anti-Stokes-shifted emission after annihilation of two metastable triplet excitons of an emitter dye and the formation of a fluorescent singlet state ...

AMER CHEMICAL SOC2021

Using flow cytometry and light-induced fluorescence to characterize the variability and characteristics of bioaerosols in springtime in Metro Atlanta, Georgia

Athanasios Nenes

The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation, and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large ...

Copernicus GmbH2020

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Michelle Susanne Frei

Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...

EPFL2020

A multi-scale time-resolved study of photoactivated dynamics in 5-benzyl uracil, a model for DNA/protein interactions

Majed Chergui, Enrico Pomarico, Mohammadhassan Valadan

We combine fluorescence up-conversion and time correlated single photon counting experiments to investigate the 5-benzyl uracil excited state dynamics in methanol from 100 fs up to several ns. This molecule has been proposed as a model for DNA/protein inte ...

2019

A Cell Atlas for the Mouse Brain

Henry Markram, Daniel Keller, Marc-Oliver Gewaltig

Despite vast numbers of studies of stained cells in the mouse brain, no current brain atlas provides region-by-region neuron counts. In fact, neuron numbers are only available for about 4% of brain of regions and estimates often vary by as much as 3-fold. ...

2018

Microfluidic staining technology and automated image processing for fast and accurate tissue-based diagnostics

Diego Gabriel Dupouy

The development of microfluidics has led to significant improvements in many areas of in vitro diagnostics. This thesis proposes the use of microfluidic device technology in the field of anatomic pathology, leading to faster and more accurate tissue-based ...

EPFL2016

SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

Kai Johnsson, Luc Reymond, Alberto Schena, Grazvydas Lukinavicius

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utilit ...

Nature Research2015

Influence of DNA Binding Dyes on Bare DNA Structure Studied with Atomic Force Microscopy

Giovanni Dietler, Aleksandre Japaridze, Aleksandr Benke, Sylvain Renevey

Fluorescent dyes are widely used for staining and visualization of DNA in optical microscopy based methods. Even though for some dyes the mechanism of binding is known, how this binding affects DNA remains poorly understood. Here we present a novel experim ...

American Chemical Society2015

Pattern-based sensing of aminoglycosides with fluorescent amphiphiles

Rosario Scopelliti, Kay Severin, Ziya Köstereli

A mixture of two amphiphiles with fluorescent head groups can be used as a sensing ensemble for the pattern-based analysis of aminoglycoside antibiotics. In buffered aqueous solution, the amphiphiles form a dynamic mixture of micellar aggregates. In the pr ...

Royal Soc Chemistry2014

Is There a Need for Smooth Muscle Cell Transplantation in Urethral Reconstruction?

Peter Frey

Background: Hypospadias and urethral strictures are conditions requiring additional tissue for reconstruction. Due to a limited source of tissue, autologous skin and oral mucosa are frequently used. However, long-term follow-up studies demonstrated signifi ...

Mary Ann Liebert, Inc2014

Live Intracellular Super-Resolution Imaging Using Site-Specific Stains

Suliana Manley, Lina Carlini

Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological feature ...

American Chemical Society2013

Cell shape-dependent early responses of fibroblasts to cyclic strain

Jürgen Brugger, Mattia Marelli

Randomly spread fibroblasts on fibronectin-coated elastomeric membranes respond to cyclic strain by a varying degree of focal adhesion assembly and actin reorganization. We speculated that the individual shape of the cells, which is linked to cytoskeletal ...

Elsevier2013

Simulated biological cells for receptor counting in fluorescence imaging

Giovanni De Micheli, Sandro Carrara, Julien Michel Ghaye

Digital image processing and epi-fluorescence microscopy provide one of the main and basic tools for living biological cells analysis and studying. Developing, testing and comparing those image processing methods properly require the use of a controlled en ...

2012

Effect of Sensitizer Adsorption Temperature on the Performance of Dye-Sensitized Solar Cells

Michael Graetzel, Mohammad Khaja Nazeeruddin, Shaik Mohammed Zakeeruddin, Jean-David Décoppet, Pascal Comte, Peng Wang

Employing a mesoscopic titania photoanode whose bilayer structure was judiciously selected to fit the optoelectronic characteristics of the Ru-based heteroleptic complex Na-cis-Ru(4,4'-(5-hexyltiophen-2-yl)-2,2'-bipyridine)(4-carboxylic-acid-4'-carboxylate ...

2011

Examining architectures of photoanode-photovoltaic tandem cells for solar water splitting

Michael Graetzel, Kevin Sivula, Jun Ho Yum, Florian Le Formal, Maurin Cornuz, Jérémie Minh-Châu Brillet

Given the limitations of the materials available for photoelectrochemical water splitting, a multiphoton (tandem) approach is required to convert solar energy into hydrogen efficiently and durably. Here we investigate a promising system consisting of a hem ...

Cambridge University Press2010

Compressed collagen gel: a novel scaffold for human bladder cells

Florian Maria Wurm, Jeffrey Alan Hubbell, Peter Frey

Collagen is highly conserved across species and has been used extensively for tissue regeneration; however, its mechanical properties are limited. A recent advance using plastic compression of collagen gels to achieve much higher concentrations significant ...

Wiley-Blackwell2010

Smooth muscle Notch1 mediates neointimal formation after vascular injury

Freddy Radtke, Yiming Li

Notch1 regulates binary cell fate determination and is critical for angiogenesis and cardiovascular development. However, the pathophysiological role of Notch1 in the postnatal period is not known. We hypothesize that Notch1 signaling in vascular smooth mu ...

2009

Vertical arrays of nanofluidic channels fabricated without nanolithography

Klaus Kern, Floriano Traversi, Kannan Balasubramanian

Vertical arrays of sealed nanofluidic channels, in which both cross-sectional dimensions are controllable down to 10 nm, were fabricated by selective side etching of a SiGe heterostructure comprised of layers of alternating Ge fractions. Capillary filling ...

2009