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Publications associées (28)

Middle-down analysis of monoclonal antibodies with electron transfer dissociation Orbitrap FTMS

Yury Tsybin, Luca Fornelli, Daniel Ayoub

The rapid growth of approved biotherapeutics, e.g., monoclonal antibodies or immunoglobulins G (IgGs), demands improved techniques for their quality control. Traditionally, proteolysis-based bottom-up mass spectrometry (MS) has been employed. However, the ...

Amer Chemical Soc2014

Extended Bottom-Up Proteomics with Secreted Aspartic Protease Sap9

Yury Tsybin, Kristina Srzentic, Unige Anna Laskay

We investigate the benefits and experimental feasibility of approaches enabling the shift from short (1.7 kDa on average) peptides in bottom-up proteomics to about twice longer (similar to 3.2 kDa on average) peptides in the so-called extended bottom-up pr ...

Elsevier Science Bv2014

Two-phase flow operational maps for multi-microchannel evaporators

John Richard Thome, Navid Borhani, Sylwia Szczukiewicz

The current paper presents new operational maps for several different multi-microchannel evaporators, with and without any inlet restrictions (micro-orifices), for the two-phase flow of refrigerants R245fa, R236fa, and R1234ze(E). The test fluids flowed in ...

Elsevier2013

Practical Considerations for Improving the Productivity of Mass Spectrometry-based Proteomics

Yury Tsybin, Anton Kozhinov, Luca Fornelli, Kristina Srzentic, Unige Anna Laskay, Oxana Upir

Mass spectrometry (MS) is currently the most sensitive and selective analytical technique for routine peptide and protein structure analysis. Top-down proteomics is based on tandem mass spectrometry (MS/MS) of intact proteins, where multiply charged precur ...

Schweizerische Chemische Gesellschaft2013

Biochemical Membrane Lipidomics during Drosophila Development

Françoise Gisou van der Goot Grunberg, Xue Li Guan, Ernst Hafen

Lipids play critical roles in energy homeostasis, membrane structure, and signaling. Using liquid chromatography and mass spectrometry, we provide a comprehensive semiquantification of lipids during the life cycle of Drosophila melanogaster (230 glyceropho ...

Cell Press - Elsevier2013

On the utility of predictive chromatography to complement mass spectrometry based intact protein identification

Yury Tsybin, Hisham Ben Hamidane

The amino acid sequence determines the individual protein three-dimensional structure and its functioning in an organism. Therefore, "reading" a protein sequence and determining its changes due to mutations or post-translational modifications is one of the ...

Springer-Verlag2012

Proteolysis in microfluidic droplets: an approach to interface protein separation and peptide mass spectrometry

Hubert Girault, Liang Qiao

A versatile microreactor protocol based on microfluidic droplets has been developed for on-line protein digestion. Proteins separated by liquid chromatography are fractionated in water-in-oil droplets and digested in sequence. The microfluidic reactor acts ...

Royal Society of Chemistry2012

Robust algorithm for aligning two-dimensional chromatograms

Jeremy Samuel Arey, Jonas Gros, Petros Dimitriou Christidis, Rebecca Rutler, Deedar Nabi

Comprehensive, two-dimensional gas chromatography (GC x GC) chromatogram's typically exhibit run-to-run retention time variability. Chromatogram alignment is often a desirable step prior to further analysis of the data, for example, in studies of environme ...

Amer Chemical Soc2012

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