Publications associées à Intégrine

Selective integrin targeting with DNA-based nanomaterials

Targeting cells specific to type and state remains a challenge in developing effective therapies, sensitive diagnostics, also robust and versatile tissue engineering. A promising strategy is to focuses on improving the inherent selectivity of the targeting ...

EPFL2022

Inhibition of SRC-mediated integrin signaling in bone marrow niche enhances hematopoietic stem cell function

Jörg Hülsken

Interaction with microenvironmental factors is crucial for the regulation of hematopoietic stem cell (HSC) function. Stroma derived factor (SDF)-1 alpha supports HSCs in the quiescent state and is central to the homing of transplanted HSCs. Here, we show t ...

CELL PRESS2022

Integrin-Mediated Adhesion in the Unicellular Holozoan Capsaspora owczarzaki

Omaya Pierre Dudin

In animals, cell-matrix adhesions are essential for cell migration, tissue organization, and differentiation, which have central roles in embryonic development [1-6]. Integrins are the major cell surface adhesion receptors mediating cell-matrix adhesion in ...

CELL PRESS2020

Standardization and Validation of Fluorescence-Based Quantitative Assay to Study Human Platelet Adhesion to Extracellular-Matrix in a 384-Well Plate

Nikolaos Stergiopoulos, Augusto Martins Lima, Tom Yohan Abraham Lachkar, François Cuenot, Cindy Marie Elodie Khawand, Damian Salim Saint Auguste

Platelets play a crucial role in the immunological response and are involved in the pathological settings of vascular diseases, and their adhesion to the extracellular matrix is important to bring leukocytes close to the endothelial cells and to form and s ...

2020

Adhesion of a tape loop

Harmeet Singh

In this work, we revisit experimentally and theoretically the mechanics of a tape loop. Using primarily elastic materials (polydimethylsiloxane, PDMS, or polycarbonate, PC) and confocal microscopy, we monitor the shape as well as the applied forces during ...

ROYAL SOC CHEMISTRY2020

Ligand Binding to the Collagen VI Receptor Triggers a Talin-to-RhoA Switch that Regulates Receptor Endocytosis

Françoise Gisou van der Goot Grunberg, Matteo Dal Peraro, Laurence Gouzi Abrami, Luciano Andres Abriata, Béatrice Kunz, Shixu Yan

Capillary morphogenesis gene 2 (CMG2/ANTXR2) is a cell surface receptor for both collagen VI and anthrax toxin. Biallelic loss-of-function mutations in CMG2 lead to a severe condition, hyaline fibromatosis syndrome (HFS). We have here dissected a network o ...

Cell Press - Elsevier2020

The Periostin/Integrin-alpha v Axis Regulates the Size of Hematopoietic Stem Cell Pool in the Fetal Liver

Jörg Hülsken

We earlier showed that outside-in integrin signaling through POSTN-ITGAV interaction plays an important role in regulating adult he-matopoietic stem cell (HSC) quiescence. Here, we show that Itgav deletion results in increased frequency of phenotypic HSCs ...

2020

Structural basis of Focal Adhesion Kinase activation on lipid membranes

Henning Paul-Julius Stahlberg, Frauke Gräter

Focal adhesion kinase (FAK) is a key component of the membrane proximal signaling layer in focal adhesion complexes, regulating important cellular processes, including cell migration, proliferation, and survival. In the cytosol, FAK adopts an autoinhibited ...

2020

Phosphoregulated orthogonal signal transduction in mammalian cells

Leo Scheller, Maysam Mansouri

Orthogonal tools for controlling protein function by post-translational modifications open up new possibilities for protein circuit engineering in synthetic biology. Phosphoregulation is a key mechanism of signal processing in all kingdoms of life, but too ...

Nature Research2020

A model for 3D deformation and reconstruction of contractile microtissues

Mahmut Selman Sakar, Erik Mailand, Jaemin Kim, Nikolaos Bouklas

Tissue morphogenesis and regeneration are essentially mechanical processes that involve coordination of cellular forces, production and structural remodeling of extracellular matrix (ECM), and cell migration. Discovering the principles of cell–ECM interact ...

2020

Bladder Cancer Photodynamic Therapeutic Agent with Off-On Magnetic Resonance Imaging Enhancement

Jean-Claude Bünzli, Chen Xie

Acting as an extracellular matrix protein receptor, alpha(v)beta(3) integrin becomes an appealing target for photodynamic therapy of bladder cancer. Here, specific alpha(v)beta(3)-integrin-targeting porphyrinato-gadolinium complexes (Gd-PEG-R-n) are demons ...

WILEY2019

Integrin-Mediated Macrophage Adhesion Promotes Lymphovascular Dissemination in Breast Cancer

Michele De Palma, Sina Nassiri, Mario Leonardo Squadrito

Lymphatic vasculature is crucial for metastasis in triple-negative breast cancer (TNBC); however, cellular and molecular drivers controlling lymphovascular metastasis are poorly understood. We define a macrophage-dependent signaling cascade that facilitate ...

2019

Atroxlysin-III, A Metalloproteinase from the Venom of the Peruvian Pit Viper Snake Bothrops atrox (Jergon) Induces Glycoprotein VI Shedding and Impairs Platelet Function

Augusto Martins Lima

Atroxlysin-III (Atr-III) was purified from the venom of Bothrops atrox. This 56-kDa protein bears N-linked glycoconjugates and is a P-III hemorrhagic metalloproteinase. Its cDNA-deduced amino acid sequence reveals a multidomain structure including a propro ...

2019

Adamts18 Function in Mammary Gland Development

Dalya Ataca

Ovarian hormones control mammary gland development and impinge on breast carcinogenesis acting via their cognate receptors in the luminal epithelium. The extracellular matrix has a key role in physiology and disease but how the two are linked is poorly und ...

EPFL2019

Label-Free Quantification Proteomics for the Identification of Mesenchymal Stromal Cell Matrisome Inside 3D Poly(Ethylene Glycol) Hydrogels

Matthias Lütolf, Yannick Robert Devaud, Martin Ehrbar

Cells modulate the functional properties of their environment by depositing extracellular matrix (ECM) proteins during biological processes in vivo and in vitro. Despite the ECMs central role in tissue formation, its quantification in hydrogels like Matrig ...

WILEY2018

Investigating Focal Adhesion Substructures by Localization Microscopy

Aleksandra Radenovic, Daniel Sage, Lely Feletti, Hendrik Georges Deschout

Cells rely on focal adhesions (FAs) to carry out a variety of important tasks, including motion, environmental sensing, and adhesion to the extracellular matrix. Although attaining a fundamental characterization of FAs is a compelling goal, their extensive ...

Elsevier2017

The matricellular protein CYR61 interferes with normal pancreatic islets architecture and promotes pancreatic neuroendocrine tumor progression

Jelena Zaric, Yu-Ting Huang

The significance of matricellular proteins during development and cancer progression is widely recognized. However, how these proteins actively contribute to physiological development and pathological cancer progression is only partially elucidated. In thi ...

Impact Journals Llc2016

Outside-in integrin signalling regulates haematopoietic stem cell function via Periostin-Itgav axis

Jörg Hülsken, Albert Santamaria Martinez

Integrins play an important role in haematopoietic stem cell (HSC) maintenance in the bone marrow niche. Here, we demonstrate that Periostin (Postn) via interaction with Integrin-alpha v (Itgav) regulates HSC proliferation. Systemic deletion of Postn resul ...

Nature Publishing Group2016

The Molecular Mechanism of P2Y 1 Receptor Activation

Horst Vogel, Shuguang Yuan

Human purinergic Gprotein-coupled receptor P2Y(1) (P2Y(1)R) is activated by adenosine 5'-diphosphate (ADP) to induce platelet activation and thereby serves as an important antithrombotic drug target. Crystal structures of P2Y(1)R revealed that one ligand ( ...

Wiley-Blackwell2016

A Gating Mechanism of the Serotonin 5-HT3 Receptor

Horst Vogel, Shuguang Yuan

Our recently solved high-resolution structure of the serotonin 5-HT3 receptor (5-HT3R) delivered the first detailed structural insights for a mammalian pentameric ligand-gated ion channel. Based on this structure, we here performed a total of 2.8-μs all-at ...

Cell Press2016