Publications associées à Levure de boulanger

Quantitative characterization of the inorganic phosphate gene regulatory networks in S. cerevisiae and bottom up engineering an orthogonal gene network

Gene regulatory networks (GRNs) play a crucial role in an organism's response to changing environmental conditions. Cellular behaviors typically result from the integration of multiple gene outputs, and their regulation often demands precise control of num ...

EPFL2024

Modulation of the nanoscale motion rate of Candida albicans by X-rays

Sandor Kasas, María Inés Villalba

IntroductionPatients undergoing cancer treatment by radiation therapy commonly develop Candida albicans infections (candidiasis). Such infections are generally treated by antifungals that unfortunately also induce numerous secondary effects in the patient. ...

Frontiers Media SA2023

Mitochondrial nanomotion measured by optical microscopy

Sandor Kasas, María Inés Villalba, Priyanka Parmar

Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and de ...

Frontiers Media SA2023

SLC25A51 is a mammalian mitochondrial NAD(+) transporter

Kai Johnsson, Liang Wang

Mitochondria require nicotinamide adenine dinucleotide (NAD(+)) to carry out the fundamental processes that fuel respiration and mediate cellular energy transduction. Mitochondrial NAD(+) transporters have been identified in yeast and plants(1,2), but thei ...

NATURE RESEARCH2020

Modeling, predicting and mining metabolism at atom-level resolution

Jasmin Maria Hafner

Living organisms can catalyze many thousands of biochemical reactions that they use to convert energy and matter, which provides them with the essentials for life. The sum of these chemical reactions happening in an organism is called metabo-lism. Understa ...

EPFL2020

Adaptive damage retention mechanism enables healthier yeast population

Riccardo Dainese

During cytokinesis in budding yeast (Saccharomyces cerevisiae) damaged proteins are distributed asymmetrically between the daughter and the mother cell. Retention of damaged proteins is a crucial mechanism ensuring a healthy daughter cell with full replica ...

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD2019

The yeast C/D box snoRNA U14 adopts a "weak" K-turn like conformation recognized by the Snu13 core protein in solution

Benjamin Marcel Daniel Rothé

Non-coding RNAs associate with proteins to form ribonucleoproteins (RNPs), such as ribosome, box C/D snoRNPs, H/ACA snoRNPs, ribonuclease P, telomerase and spliceosome to ensure cell viability. The assembly of these RNA-protein complexes relies on the abil ...

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER2019

Atomic Force Microscopy of Biological Systems: Quantitative Imaging and Nanomotion Detection

Petar Stupar

The core of this thesis is the application of different modalities of atomic force microscopy to study living systems. After a brief introduction, quantitative imaging state of the art is presented and the applications for mammalian, plant, bacterial, and ...

EPFL2018

Modulation of the endogenous production of protoporphyrin IX in a yeast - based model organism

Georges Wagnières, Emmanuel Louis Arthur Gerelli, Jaroslava Joniová

The main aim of this study was to assess conditions at which simple yeast-based model organism produces maximal levels of protoporphyrin IX (PpIX) after an exogenous administration of its precursor, 5-aminolevulinic acid (ALA), and the ferrous-ion chelator ...

Spie-Int Soc Optical Engineering2017

An automated microreactor for semi-continuous biosensor measurements

Philippe Renaud, Harald Van Lintel, Nina Buffi, Jan Roelof van der Meer

Living bacteria or yeast cells are frequently used as bioreporters for the detection of specific chemical analytes or conditions of sample toxicity. In particular, bacteria or yeast equipped with synthetic gene circuitry that allows the production of a rel ...

Royal Society of Chemistry2016

Optimization and characterization of the endogenous production of protoporphyrin IX in a yeast model

Georges Wagnières, Emmanuel Louis Arthur Gerelli, Jaroslava Joniová, Matthieu Philippe Emmanuel Zellweger

The availability of reproducible, convenient, and inexpensive model organisms able to generate predictable levels of endogenous porphyrins, including protoporphyrin IX (PpIX), is essential in photomedicine research. Saccharomyces cerevisiae produces endoge ...

SPIE Society of Photo-optical Instrumentation Engineers2016

Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces cerevisiae by photo-Fenton at near-neutral pH

César Pulgarin, Sami Rtimi, Stefanos Giannakis, Cristina del Socorro Lonfat

In this work, the antimicrobial effects of the photo-Fenton process on yeast cells were tested (Saccharomyces cerevisiae), an essential eukaryotic unicellular model of living cells. Near-neutral pH was used in all studies, while iron sulfate and iron citra ...

Elsevier2016

Live yeasts enhance fibre degradation in the cow rumen through an increase in plant substrate colonization by fibrolytic bacteria and fungi

AimsTo monitor the effect of a live yeast additive on feedstuff colonization by targeted fibrolytic micro-organisms and fibre degradation in the cow rumen. Methods and ResultsAbundance of adhering fibrolytic bacteria and fungi on feedstuffs incubated in sa ...

Wiley-Blackwell2016

Adaptation on a genomic scale

Jacques Fellay, Istvan Bartha

Sequencing the genome of Candida albicans as it evolves in a patient reveals the genetic changes that allow the yeast to adapt to its environment. ...

2015

Efficient Shape Priors for Spline-Based Snakes

Michaël Unser, Daniel Andreas Schmitter, Ricard Delgado Gonzalo, Virginie Sophie Uhlmann

Parametric active contours are an attractive approach for image segmentation, thanks to their computational efficiency. They are driven by application-dependent energies that reflect the prior knowledge on the object to be segmented. We propose an energy i ...

2015

Molecular Mechanism of Flocculation Self-Recognition in Yeast and Its Role in Mating and Survival

Sandor Kasas, Livan Bladimir Alonso Sarduy, Jens Nielsen

We studied the flocculation mechanism at the molecular level by determining the atomic structures of N-Flo1p and N-Lg-Flo1p in complex with their ligands. We show that they have similar ligand binding mechanisms but distinct carbohydrate specificities and ...

American Society for Microbiology2015

Merging microfluidics and micro-array concepts: from molecular to nematode-based bioassays

Matteo Cornaglia

Essential in biomedical research is the necessity of gathering statistically relevant data about large populations of specific biological entities, e.g. organisms, cells or molecules, while preserving detailed information about each single entity under in ...

EPFL2015

Mathematical models and computational methods for the analysis of genome-scale protein synthesis

Julien Racle

Proteins are a ubiquitous and indispensable element for every living organism, from simple bacteria to mammals. Already in the simplest organisms, there exist some thousands of different protein species that take up a great variety of structures, and thus ...

EPFL2014

Expression in Yeast Links Field Polymorphisms in PfATP6 to in Vitro Artemisinin Resistance and Identifies New Inhibitor Classes

Serena Pulcini

Background. The mechanism of action of artemisinins against malaria is unclear, despite their widespread use in combination therapies and the emergence of resistance. Results. Here, we report expression of PfATP6 (a SERCA pump) in yeast and demonstrate its ...

Oxford University Press2013

Structure and properties of La-modified Na0.5Bi0.5TiO3 at ambient and elevated temperatures

Dragan Damjanovic

The crystal structure and property changes of sodium bismuth titanate (Na0.5Bi0.5TiO3, NBT) piezoelectric ceramics are reported as a function of La modification (0.5-2.0 at. %) and increasing temperature using high resolution x-ray diffraction, permittivit ...

American Institute of Physics2012