Publications associées à Nematoda

Microfluidic culture platforms for C. elegans bacterial interaction and digestion studies at single-organism resolution

Increasing interest in understanding fundamental biological processes, among which aging and nutrition, has led to the study of simple and powerful model organisms, as the round-worm Caenorhabditis elegans (C. elegans). The nematode, selected by Sydney Bre ...

EPFL2021

Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform

Martinus Gijs, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hüseyin Baris Atakan, Tunç Alkanat

The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the sev ...

2020

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Rongrong Xiang, Elena Katsyuba, Hüseyin Baris Atakan

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

Microfluidic and micromechanical in vivo studies of Caenorhabditis elegans

Roger Philippe Krenger

Caenorhabditis elegans is one of the most attractive model organisms in biomedical research for understanding human diseases and for drug testing at a whole-organism level, since many biological pathways and genes have been conserved between itself and hum ...

EPFL2019

Diet modulates cecum bacterial diversity and physiological phenotypes across the BXD mouse genetic reference population

Johan Auwerx, Evan Graehl Williams

The BXD family has become one of the preeminent genetic reference populations to understand the genetic and environmental control of phenotypic variation. Here we evaluate the responses to different levels of fat in the diet using both chow diet (CD, 13-18 ...

2019

Long-term imaging, automated high-content phenotyping and sorting of C. elegans larvae and embryos using microfluidic platforms

Hüseyin Baris Atakan

C. elegans is frequently studied to address fundamental biological questions motivated by its short life cycle, small size, hermaphrodite behavior and well-annotated genome. In this work, we present novel microfluidic platforms that support long-term cultu ...

EPFL2019

Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment

Pierre Gönczy, Alexandra Bezler, Frédéric Schütz, Laurent Keller, Raffaella Conconi

RNA interference (RNAi) related pathways are essential for germline development and fertility in metazoa and can contribute to inter- and trans-generational inheritance. In the nematode Caenorhabditis elegans, environmental double-stranded RNA provided by ...

2019

Automated high-content phenotyping from the first larval stage till the onset of adulthood of the nematode Caenorhabditis elegans

Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan

The nematode Caenorhabditis elegans is increasingly used as a model for human biology. However, in vivo culturing platforms for C. elegans allowing high-content phenotyping during their life cycle in an automated fashion are lacking so far. Here, a multipl ...

ROYAL SOC CHEMISTRY2019

8-Channel Single Embryo Pipette For Accurate C-Elegans Bioassays

Martinus Gijs, Thomas Lehnert, Li Dong, Jingwei Zhang

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or bioch ...

IEEE2018

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Li Dong

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

EPFL2018

C. Elegans Immobilization Using Deformable Microfluidics For In Vivo Studies Of Early Embryogenesis And Intestinal Microbiota

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong, Xiaopeng Huang, Jingwei Zhang

The genome of the nematode Caenorhabditis elegans shows far-reaching similarities to that of humans. C. elegans is therefore considered as a powerful model organism in biological research related to humans, for instance for the study of human diseases. Suc ...

Ieee2017

Versatile size-dependent sorting of C-elegans nematodes and embryos using a tunable microfluidic filter structure

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

The roundworm Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Its life cycle consists of an embryo stage, four larval stages that can be clearly distinguis ...

Royal Soc Chemistry2016

On-chip microfluidic biocommunication assay for studying male-induced demise in C. elegans hermaphrodites

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

Like other animals, C. elegans nematodes have the ability to socially interact and to communicate through exchange and sensing of small soluble signaling compounds that help them cope with complex environmental conditions. For the time being, worm biocommu ...

Royal Soc Chemistry2016

Size-dependent sorting of C. elegans nematodes through an adjustable microfluidic filter structure

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

We introduce a new and simple microfluidic sorting device based on adjustable filter structures that can be easily tuned by external pressure for a specific size-dependent C. elegans worm sorting experiment. A single device can be used for selecting and so ...

2016

Hydrology and density feedbacks control the ecology of intermediate hosts of schistosomiasis across habitats in seasonal climates

Andrea Rinaldo, Enrico Bertuzzo, Lorenzo Mari, Natalie Claire Ceperley, Theophile Mande, Francisco Javier Perez Saez, Marino Gatto

We report about field and theoretical studies on the ecology of the aquatic snails (Bulinus spp. and Biomphalaria pfeifferi) that serve as obligate intermediate hosts in the complex life cycle of the parasites causing human schistosomiasis. Snail abundance ...

Natl Acad Sciences2016

Exploiting Old Pathogens to Create New Therapeutics

Nicola Harris, Tiffany Babette Angélique Bouchery

Intestinal worms are well known for their potent immuno-modulatory capacity. In a recent study, Navarro et al. (2016) identify a secreted hookworm protein that can suppress allergic responses in both mice and humans. This represents an exciting strategy fo ...

Cell Press2016

Merging microfluidics and micro-array concepts: from molecular to nematode-based bioassays

Matteo Cornaglia

Essential in biomedical research is the necessity of gathering statistically relevant data about large populations of specific biological entities, e.g. organisms, cells or molecules, while preserving detailed information about each single entity under in ...

EPFL2015

The Intestinal Microbiota Contributes to the Ability of Helminths to Modulate Allergic Inflammation

Jacques Rougemont, Laure Menin, Nicola Harris, Mario Michael Zaiss, Ilaria Mosconi, Alessandra Piersigilli, Benjamin John Marsland, Lalit Kumar Dubey, Alexis Rapin, Luc Xavier Marie Lebon, Andrew J. Macpherson

Intestinal helminths are potent regulators of their host's immune system and can ameliorate inflammatory diseases such as allergic asthma. In the present study we have assessed whether this anti-inflammatory activity was purely intrinsic to helminths, or w ...

Elsevier2015

Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry

Pierre Gönczy, Virginie Hamel, Georgios Hatzopoulos, Paul Guichard, Isabelle Fluckiger

Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas ...

2013

Centrosome duplication and nematodes: recent insights from an old relationship

Pierre Gönczy

Centrosome duplication is required for proper cell division, and centriole formation is a key step in this process. This review discusses recent studies in C. elegans that have identified five core proteins required for centriole formation, thus shedding l ...

Cell Press - Elsevier2005