Publications associées à Romanowsky stain

Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes

Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures i ...

2023

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Demetri Psaltis, Joowon Lim, Daniele Pirone

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

Nature Publishing Group2022

HISTOBREAST, a collection of brightfield microscopy images of Haematoxylin and Eosin stained breast tissue

Tiberiu Totu

Modern histopathology workflows rely on the digitization of histology slides. The quality of the resulting digital representations, in the form of histology slide image mosaics, depends on various specific acquisition conditions and on the image processing ...

2020

Dual-Activatable Cell Tracker for Controlled and Prolonged Single-Cell Labeling

Pablo Rivera Fuentes, Salome Riccarda Püntener

Cell trackers are fluorescent chemical tools that facilitate imaging and tracking cells within live organisms. Despite their versatility, these dyes lack specificity, tend to leak outside of the cell, and stain neighboring cells. Here, we report a dual-act ...

AMER CHEMICAL SOC2020

High-content, cell-by-cell assessment of HER2 overexpression and amplification: a tool for intratumoral heterogeneity detection in breast cancer

Martinus Gijs, Daniel Migliozzi, Huu Tuan Nguyen

Immunohistochemistry and fluorescence in situ hybridization are the two standard methods for human epidermal growth factor receptor 2 (HER2) assessment. However, they have severe limitations to assess quantitatively intratumoral heterogeneity (ITH) when mu ...

NATURE PUBLISHING GROUP2019

Design and Synthesis of Gatekeeper Coated Dendritic Silica/Titania Mesoporous Nanoparticles with Sustained and Controlled Drug Release Properties for Targeted Synergetic Chemo-Sonodynamic Therapy

Saba Rezakhani, Samira Malekmohammadi

New dendritic silica/titania mesoporous nanoparticles (DSTNs) loaded with curcumin (CUR) were synthesized and coated with polyethylenimine-folic acid groups (PEI-FA) for an ultrasound (US)-triggered drug release and combined chemo-sonodynamic therapy. The ...

2019

Development of Sample-Adaptable Holders for Lightsheet Microscopy

Thierry Laroche, Olivier Burri, Arne Seitz, Lalit Kumar Dubey

Multi-user core microscopy facilities are often faced with the challenge to adapt or modify existing instruments. This is essential in order to fulfill the requirements of the user community, who wants to image a wide range of model organisms with varying ...

2019

Gap-Plasmon-Enhanced High-Spatial-Resolution Imaging by Photothermal-Induced Resonance in the Visible Range

Giovanni Dietler, Serguei Sekatski, Anton Smirnov, Jiangtao Zhou

Chemical characterization at the nanoscale is of significant importance for many applications in physics, analytical chemistry, material science, and biology. Despite the intensive studies in the infrared range, high-spatial resolution and high-sensitivity ...

AMER CHEMICAL SOC2019

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Stewart Cole, Charlotte Avanzi, Philippe Busso

Background ...

PUBLIC LIBRARY SCIENCE2018

Recent Advances of Malaria Parasites Detection Systems Based on Mathematical Morphology

Michel Kocher

Malaria is an epidemic health disease and a rapid, accurate diagnosis is necessary for proper intervention. Generally, pathologists visually examine blood stained slides for malaria diagnosis. Nevertheless, this kind of visual inspection is subjective, err ...

2018

Cu-decorated Raschig-TiO2 rings inducing MB repetitive discoloration without release of Cu-ions under solar light

César Pulgarin, Michaël Bensimon, Juan Kiwi, Rosendo Sanjines, Laura Victoria Suárez Murillo, Helena Luisa Teixido Pedarros

The methylene blue (MB) discoloration on innovative photocatalysts made up of Raschig-rings coated by pre-formed TiO2 nanoparticles decorated with CuOx (RR@TiO2-Cu) is presented. The MB-discoloration was drastically accelerated by the subsequent addition o ...

Elsevier Sci Ltd2017

Polystyrene CuO/Cu2O uniform films inducing MB-degradation under sunlight

César Pulgarin, Juan Kiwi, Sami Rtimi, Oualid Baghriche

This study reports on a Cu-sputtered film on polystyrene (PS) leading to the discoloration/degradation of methylene blue (MB) under low intensity solar simulated irradiation. Direct current magnetron sputtering (DCMS) was used to graft uniform, adhesive Cu ...

Elsevier2017

Innovative photo-Fenton catalysis by PE-FeOx films leading to methylene blue (MB) degradation: Kinetics, surface properties and mechanism

César Pulgarin, Juan Kiwi, Laura Victoria Suárez Murillo, Rosendo Sanjines, Huiyu Dong

This study reports an innovative preparation polyethylene-FeOx (PE-FeOx films) leading to the degradation of the methylene blue (MB) dye under low intensity sunlight/visible light (>400 nm). The intermediate highly oxidative radicals generated by PE-FeOx l ...

Elsevier2016

Preparation, kinetics, mechanism and properties of semi-transparent photocatalytic stable films active in dye degradation

César Pulgarin, Juan Kiwi, Laura Victoria Suárez Murillo, Christophe Roussel

The photocatalytic activity, mechanism and characterization of polyethylene-TiO2 films (PE-TiO2) during the discoloration/degradation of methylene blue (MB) are reported in this study. PE-TiO2 films were prepared at moderate temperatures (similar to 90 deg ...

Elsevier2016

Scalable Unsupervised Domain Adaptation for Electron Microscopy

Pascal Fua, Mathieu Salzmann, Carlos Joaquin Becker, Róger Bermúdez Chacón

While Machine Learning algorithms are key to automating organelle segmentation in large EM stacks, they require annotated data, which is hard to come by in sufficient quantities. Furthermore, images acquired from one part of the brain are not always repres ...

2016

Influence of DNA Binding Dyes on Bare DNA Structure Studied with Atomic Force Microscopy

Giovanni Dietler, Aleksandre Japaridze, Aleksandr Benke, Sylvain Renevey

Fluorescent dyes are widely used for staining and visualization of DNA in optical microscopy based methods. Even though for some dyes the mechanism of binding is known, how this binding affects DNA remains poorly understood. Here we present a novel experim ...

American Chemical Society2015

Kinetics and mechanism for transparent polyethylene-TiO2 films mediated self-cleaning leading to MB dye discoloration under sunlight irradiation

César Pulgarin, Juan Kiwi, Sami Rtimi, Rosendo Sanjines

Polyethylene-TiO2 (from now on PE-TiO2) transparent, non-scattering sputtered films have been found effective in the discoloration of methylene blue (MB) under low intensity solar simulated light. By Fourier transform attenuated infrared spectroscopy (ATR- ...

Elsevier Science Bv2015

Effect of fuels on combustion synthesis of TiO2 - Towards efficient photocatalysts for methylene blue oxidation and Cr (VI) reduction under natural sunlight

Nicolas Xanthopoulos

A facile synthesis of carbon and nitrogen co-doped nano-TiO2 with pure anatase phase and high surface area was achieved by using combustion synthesis without the need of surfactants in a single step approach. Organic fuels such as hexamine, triethyl amine ...

Elsevier Science Sa2013

Contrast Enhancement in Optical Coherence Microscopy

Christophe Pache

Optical Coherence Microscopy (OCM) is a three-dimensional imaging technique that provides cross-sectional views of the subsurface microstructure of biological tissue, with a high axial and lateral resolution. In OCM, a low time coherence light source is sp ...

EPFL2012

A Study of Synthesis & [and] Performance of Flame-Made Semiconducting BiVO4 Nanoparticles for the Photocatalytic Degradation of Aqueous Organic Pollutants

Nikola Castillo

Semiconductor photocatalysis is an Advanced Oxidation Technology that uses light as a source of energy to accelerate the degradation of harmful organic pollutants. Current research addresses the development of photocatalysts capable of harvesting visible l ...

EPFL2011