Publications associées à Matériel péricentriolaire

Dissecting centriole fate in C. elegans development

The presence of centrioles is critical for fundamental cell and developmental processes, including polarity, migration and division. Although centrioles are present in most proliferating cells, they are thought to vanish after cell cycle exit in some diffe ...

EPFL2022

Architecture of the centriole cartwheel‐containing region revealed by cryo‐electron tomography

Henning Paul-Julius Stahlberg, Paul Guichard, Virginie Hamel

Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture ...

2020

A helical inner scaffold provides a structural basis for centriole cohesion

Henning Paul-Julius Stahlberg, Davide Gambarotto, Paul Guichard, Virginie Hamel

The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary ...

2020

Multicolor single-particle reconstruction of protein complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

2018

Centriole Biogenesis: From Identifying the Characters to Understanding the Plot

Pierre Gönczy, Niccolo Banterle

The centriole is a beautiful microtubule-based organelle that is critical for the proper execution of many fundamental cellular processes, including polarity, motility, and division. Centriole biogenesis, the making of this miniature architectural wonder, ...

Annual Reviews2017

Purification of centrosomes from mammalian cell lines

Paul Guichard

Centrosomes act as the main microtubule-organizing centre of animal cells and play critical roles in the cell, such as mitotic spindle organization, cell polarity, and motility. They are composed of two barrel-shaped structures, the centrioles, surrounded ...

Elsevier Academic Press Inc2015

Mechanisms of differential centriole inheritance in C. elegans

Lukas von Tobel

Centrosomes are the principal microtubule organizing center (MTOC) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated to ensure bipolar spindle formation and thus fait ...

EPFL2014

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

Pierre Gönczy, Davide Demurtas, Paul Guichard, Isabelle Fluckiger, Aitana Neves da Silva, Norbert Majubu

Centrioles are cylindrical microtubule-based structures whose assembly is critical for the formation of cilia, flagella, and centrosomes. The centriole proximal region harbors a cartwheel that dictates the 9-fold symmetry of centrioles. Although the cartwh ...

2013

Analysis of centriole elimination during C. elegans oogenesis

Pierre Gönczy, Graham Knott, Petr Strnad, Lukas von Tobel, Tamara Mikeladze-Dvali

Centrosomes are the principal microtubule organizing centers (MTOCs) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated, notably to ensure bipolar spindle formation an ...

2012

Towards a molecular architecture of centriole assembly

Pierre Gönczy

The centriole is an evolutionarily conserved macromolecular structure that is crucial for the formation of flagella, cilia and centrosomes. The ultrastructure of the centriole was first characterized decades ago with the advent of electron microscopy, reve ...

2012

Functional characterization of the SAS-4-related protein CPAP in centrosome biology of human cells

Gregor Kohlmaier

The centrosome is an organelle that resides at the center of most animal cells and comprises two microtubule-based centriole cylinders surrounded by pericentriolar material (PCM). The centrosome plays a fundamental role for nucleating and organizing a radi ...

EPFL2009

Phosphorylation of SAS-6 by ZYG-1 is critical for centriole formation in C. elegans embryos

Pierre Gönczy, Coralie Busso, Isabelle Fluckiger

Despite being essential for proper cell division, the mechanisms governing centrosome duplication are incompletely understood and represent an important open question in cell biology. Formation of a new centriole next to each existing one is critical for c ...

Cell Press - Elsevier2009

Overly long centrioles and defective cell division upon excess of the SAS-4-related protein CPAP

Pierre Gönczy, Gregor Kohlmaier

The centrosome is the principal microtubule organizing center (MTOC) of animal cells. Accurate centrosome duplication is fundamental for genome integrity and entails the formation of one procentriole next to each existing centriole, once per cell cycle. Th ...

2009

Mechanisms of procentriole formation

Pierre Gönczy, Petr Strnad

The centrosome comprises a pair of centrioles and associated pericentriolar material, and it is the principal microtubule-organizing centre of most animal cells. Like the genetic material, the centrosome is duplicated once and only once during the cell cyc ...

2008