Publications associées à Cytométrie

Portable impedance-sensing device for microorganism characterization in the field

A variety of biosensors have been proposed to quickly detect and measure the properties of individual microorganisms among heterogeneous populations, but challenges related to cost, portability, stability, sensitivity, and power consumption limit their app ...

Nature Portfolio2023

Portable Multi-Frequency Impedance-Sensing Device for Bacteria Classification in a Flowing Liquid

Sandro Carrara, Karim Bouzid

This paper presents a novel impedance-sensing device and its related microfluidics system developed to characterize the macroscopical properties of bacteria and microparticles. The system is based on a phase-sensitive detector with square-waves excitation ...

IEEE2022

Simplified Instrument Calibration forWide-FieldFluorescence Resonance Energy Transfer (FRET) Measured by the Sensitized Emission Method

Forster (or fluorescence) resonance energy transfer (FRET) is a quantifiable energy transfer in which a donor fluorophore nonradiatively transfers its excitation energy to an acceptor fluorophore. A change in FRET efficiency indicates a change of proximity ...

2021

Impedance-Based Single-Cell Pipetting

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Jean-Baptiste Bureau, Georges Muller, Nicolas Jean Bernard Beuchat, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here ...

SAGE PUBLICATIONS INC2020

How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study

Philippe Renaud, Jonathan Bastien Cottet, Harald Van Lintel

This paper describes a comprehensive analysis of the geometrical parameters in uencing the sensitivity of a coplanar elec- trode layout for electrical impedance ow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3 ...

2019

Impedance Spectroscopy for Single Cell Analysis and Dispensing

David Vincent Bonzon

Flow cytometry is an essential tool in biology and medicine with applications in immunodeficiency diagnostic, immunosurveillence, organ transplantation in addition to its basic research usage. Before the widespread use of the Fluorescent-Activated Cell Sor ...

EPFL2017

Microfluidic-integrated vertical electrodes employed in impedance-based cytometry : potential application in immunotherapies

Enrica Rollo

During the last decades, the growing interest for single-cell analysis has led to the creation of a number of microfluidic and lab-on-a chip (LOC) platforms for characterizing cellular samples. In that context label-free based platforms are minimally invas ...

EPFL2016

Digital microfluidics for time-resolved cytotoxicity studies on single non-adherent yeast cells

Martinus Gijs, Matteo Cornaglia

Single cell analysis (SCA) has gained increased popularity for elucidating cellular heterogeneity at genomic, proteomic and cellular levels. Flow cytometry is considered as one of the most widely used techniques to characterize single cell responses; howev ...

Royal Soc Chemistry2015

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Gerardo Turcatti, Benjamin Rappaz, Marc Chambon, Jonas Kühn, Etienne Shaffer, Jérôme Parent, Julien Mena

We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screenin ...

Mary Ann Liebert2013

Automated analysis of single stem cells in microfluidic traps

Matthias Lütolf, Alessandra Griffa, Arne Seitz, Olivier Burri, Stefan Kobel, Mukul Girotra

We report a reliable strategy to perform automated image cytometry of single (non-adherent) stem cells captured in microfluidic traps. The method rapidly segments images of an entire microfluidic chip based on the detection of horizontal edges of microflui ...

2012

Cell viability assessment by flow cytometry using yeast as cell model

Philippe Renaud, Nina Buffi, Niccolo Piacentini, Raphaël Tornay

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of ...

2011

Microfluidic array cytometer based on refractive optical tweezers for parallel trapping, imaging and sorting of individual cells

René Salathé, Horst Vogel, Fabrice Merenda, Joachim Piguet, Michael Werner

Analysis of genetic and functional variability in populations of living cells requires experimental techniques capable of monitoring cellular processes such as cell signaling of many single cells in parallel while offering the possibility to sort interesti ...

Royal Society of Chemistry2011

Optical Trapping in Microfluidic Channels for Functional and Chemical Analysis of Single Cells

Michael Werner

The physiological properties of cells are typically investigated in ensembles yielding averaged data that mask heterogeneities present in any cell population. Single-cell analysis techniques based on microfluidic "lab on a chip" (LOC) devices have recently ...

EPFL2011

Fc Block Treatment, Dead Cells Exclusion, and Cell Aggregates Discrimination Concur to Prevent Phenotypical Artifacts in the Analysis of Subpopulations of Tumor-Infiltrating CD11b(+) Myelomonocytic Cells

It is well established that cancer cells can recruit CD11b(+) myeloid cells to promote tumor angiogenesis and tumor growth. Increasing interest has emerged on the identification of subpopulations of tumor-infiltrating CD11b(+) myeloid cells using flow cyto ...

2010

Individual cells immobilization for water-borne pathogen detection and enumeration

A cell counting device has been proposed and implemented for water-borne pathogen detection for drinking water quality monitoring applications. Our approach is based on magnetically-labelled cells immobilization in a high density array of individual cell f ...

Elsevier Science, Reg Sales Off, Customer Support Dept, 655 Ave Of The Americas, New York, Ny 10010 Usa2009

Analytical expression for electric filed between two facing strip electrodes in microchannels

Philippe Renaud, Urban Seger, Olof Pontus Linderholm

An analytical solution is presented for the electric field between two facing strip electrodes situated in a microchannel, as frequently used in impedance cytometry applications. The measured change in resistance as induced by a 5 mm bead moving through th ...

2006

Device for dispensing microfluidic droplets, particularly for cytometry

Philippe Renaud

The invention relates to a device for dispensing droplets comprising a first channel (8, 10), known as the main channel, for circulating a first liquid flow, a second channel (12, 13) for circulating fluid, forming an intersection area (27) with the first ...

2005