Publications associées à Serum protein electrophoresis

Alpha1-antitrypsin improves survival in murine abdominal sepsis model by decreasing inflammation and sequestration of free heme

Background Excessive inflammation, hemolysis, and accumulation of labile heme play an essential role in the pathophysiology of multi-organ dysfunction syndrome (MODS) in sepsis. Alpha1-antitrypsin (AAT), an acute phase protein with heme binding capacity, i ...

Frontiers Media Sa2024

Human PBMCs Form Lipid Droplets in Response to Spike Proteins

Florian Maria Wurm, Guillaume Raussin

Intracellular lipid droplets (LDs) can accumulate in response to inflammation, metabolic stresses, and other physiological/pathological processes. Herein, we investigated whether spike proteins of SARS-CoV-2 induce LDs in human peripheral blood mononuclear ...

MDPI2023

Improved antibody pharmacokinetics by disruption of contiguous positive surface potential and charge reduction using alternate human framework

Andrea Pfeifer, Florence Gauye, Tariq Afroz, Monisha Ratnam

Optimal pharmacokinetic (PK) properties of therapeutic monoclonal antibodies (mAbs) are essential to achieve the desired pharmacological benefits in patients. To accomplish this, we followed an approach comprising structure-based mAb charge engineering in ...

TAYLOR & FRANCIS INC2023

Telomere protection against oxidative stress

Thu Trang Nguyen

Telomeres are nucleoprotein structures at the ends of linear chromosomes, being essential for the maintenance of genomic integrity. Telomeres have a unique structure which distinguishes chromosome termini from DNA damage sites. Shelterin complexes are the ...

EPFL2023

A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

Didier Trono, Priscilla Turelli, Florence Pojer, Charlène Mireille Raymonde Raclot, Valeria Cagno, Jérémy Campos, Céline Pellaton

The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies in the serum of an individual indicates previous infection or vaccination. However, it provides limited insight into the protective nature of this immune resp ...

AMER ASSOC ADVANCEMENT SCIENCE2021

Growth differentiation factor 15 protects against the aging-mediated systemic inflammatory response in humans and mice

Johan Auwerx, Jun Yong Kim, Ludger Jan Elzuë Goeminne, Dongryeol Ryu

Mitochondrial dysfunction is associated with aging-mediated inflammatory responses, leading to metabolic deterioration, development of insulin resistance, and type 2 diabetes. Growth differentiation factor 15 (GDF15) is an important mitokine generated in r ...

2020

An Overview of Serum Albumin Interactions with Biomedical Alloys

Stefano Mischler, Anna Neus Igual Muñoz, Oksana Klok

Understanding the interactions between biomedical alloys and body fluids is of importance for the successful and safe performance of implanted devices. Albumin, as the first protein that comes in contact with an implant surface, can determine the biocompat ...

2020

Antibody-Mediated Trapping of Helminth Larvae Requires CD11b and Fc gamma Receptor I

Arne Seitz, Romain Guiet, Duncan-Bruce Sutherland, Nicola Harris, Benjamin John Marsland, Beatrice Volpe, Julia Esser-von Bieren, Manuel Kulagin

Infections with intestinal helminths severely impact on human and veterinary health, particularly through the damage that these large parasites inflict when migrating through host tissues. Host immunity often targets the motility of tissue-migrating helmin ...

American Association of Immunologists ; Oxford University Press2015

Significance of surface charge and shell material of superparamagnetic iron oxide nanoparticle (SPION) based core/shell nanoparticles on the composition of the protein corona

Heinrich Hofmann, Usawadee Sakulkhu, Lionel André Maurizi, Morteza Mahmoudi, Géraldine Sylvie Coullerez

As nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" nanoparticles, as well as the ...

Royal Soc Chemistry2015

Temperature-Controlled Masking/Unmasking of Cell-Adhesive Cues with Poly(ethylene glycol) Methacrylate Based Brushes

Harm-Anton Klok, Solenne Desseaux

Thin, thermoresponsive polymer coatings that allow to reversibly modulate cell adhesion and detachment are attractive substrates for cell sheet engineering. Usually, this is accomplished by applying thin poly(N-isopropylacrylamide) (PNIPAM) coatings, which ...

American Chemical Society2014

Compatible Buffer for Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Hubert Girault, Natalia Gasilova, Liang Qiao, Hong-Xu Chen

A compatible buffer system for the coupling of capillary electrophoresis (CE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was developed. The employed interface consists of a robot to drive a sliver-covered separation capil ...

Royal Society of Chemistry2013

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies

Mohammad Reza Pourhaghighi

Proteomic studies require analytical methods capable of separating, identifying and quantifying thousands of proteins. Consequently, developing separation methodologies with high resolving power necessary to separate complex protein samples prior to protei ...

EPFL2012

Segmented Field OFFGEL® Electrophoresis

Hubert Girault, Julien Maillard, Fernando Cortes Salazar, Dmitry Momotenko, Elena Tobolkina

A multielectrode setup for protein OFFGEL electrophoresis that significantly improves protein separation efficiency has been developed. Here, the electric field is applied by segments between seven electrodes connected in series to six independent power su ...

Wiley-Blackwell2012

Total serum IgE quantification by microfluidic ELISA using magnetic beads

Hubert Girault, Anne-Laure Gassner

The present work reports on the quantification of total IgE in human serum using a microanalytical device whose fluidics is driven by gravity and capillary forces only. Thanks to the eight parallel microchannels in each microchip, calibration and sample an ...

Springer Verlag2012

High-sensitive protein analysis by FESI-CEMALDI-MS

Hubert Girault, Mohammad Reza Pourhaghighi

Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iont ...

Wiley-Blackwell2011

Human serum albumin binding and cytotoxicity studies of surfactant–cobalt(III) complex containing 1,10-phenanthroline ligand

Hubert van den Bergh, Georges Wagnières

The characteristics of the binding reaction of surfactant-cobalt(III) complex, cis-[Co(phen)(2)(C14H29NH2)]Cl-2 center dot 3H(2)O (phen =1,10-phenanthroline, C14H29NH2 = tetradecylamine) with human serum albumin (HSA) were studied by fluorescence and UV-vi ...

Elsevier2011

Bubble cell for magnetic bead trapping in capillary electrophoresis

Hubert Girault, Anne-Laure Gassner

A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case wi ...

Springer Verlag2011

A Novel Cytotoxic Cerium Complex: Aquatrichloridobis(1,10-phenanthroline)cerium(III) (KP776). Synthesis, Characterization, Behavior in H2O, Binding towards Biomolecules, and Antiproliferative Activity

Michael Groessl

The lanthanide complex aquatrichloridobis(1,10-phenanthroline)cerium(III) [Ce(phen)(2)(H2O)Cl-3] (KP776) was fully characterized by elemental analysis, IR-, and H-1- and C-13-NMR spectroscopy, as well as TG/DTA measurements, and its behavior in H2O, import ...

2009

Kinetics of Proteolytic Reactions in Nanoporous Materials

Hubert Girault, Liang Qiao, Hongyan Bi

Proteolysis with proteases preloaded within nanopores of porous material is a very fast process, where proteins can be digested in minutes compared to the conventional bulk enzyme reactions taking place over hours. To model this surprising phenomenon, a mo ...

American Chemical Society2009

Hypercholesterolemic Mice Exhibit Lymphatic Vessel Dysfunction and Degeneration

Melody Swartz, Joseph Rutkowski, Sai Reddy

Lymphatic vessels are essential for lipid absorption and transport. Despite increasing numbers of observations linking lymphatic vessels and lipids, little research has been devoted to address how dysregulation of lipid balance in the blood, ie, dyslipidem ...

American Society for Investigative Pathology2009