Publications associées à Culture tissulaire

First Advanced Bilayer Scaffolds for Tailored Skin Tissue Engineering Produced via Electrospinning and Melt Electrowriting

In vitro skin models are validated methods for screening cosmetics and pharmaceuticals, but still have limitations. The bilayer poly(epsilon-caprolactone) scaffold/membrane model described here overcomes some of these deficits by integrating a solution ele ...

Wiley-V C H Verlag Gmbh2024

Fluorescent fatty acid conjugates for live cell imaging of peroxisomes

Luc Reymond, Fabien Kuttler, Milena Maria Schuhmacher, Manon Sandra Bardyn, Daria Korotkova, Triana Amen

Peroxisomes are eukaryotic organelles that are essential for multiple metabolic pathways, including fatty acid oxidation, degradation of amino acids, and biosynthesis of ether lipids. Consequently, peroxisome dysfunction leads to pediatric-onset neurodegen ...

Nature Research2024

System for automated and dynamic cell culture

Julia Tischler

The present relates to a system for automated cell culture, the system comprising an inflow module, a cell culture plate and an outflow module, said inflow module being in fluid connection with the outflow module via the cell culture plate, the inflow modu ...

2023

Nanoliter Liquid Packaging in a Bioresorbable Microsystem by Additive Manufacturing and its Application as a Controlled Drug Delivery Device

Jürgen Brugger, Arnaud Bertsch, Cristina Martin Olmos, Jongeon Park

Precise packaging of nanoliter amounts of liquid in a microsystem is important for many biomedical applications. However, existing liquid encapsulation technologies have limitations in terms of liquid waste, evaporation, trapped bubbles, and liquid degrada ...

2023

Perfluorocarbon emulsion enhances MR-ARFI displacement and temperature in vitro: Evaluating the response with MRI, NMR, and hydrophone

Mohamed Farhat, Davide Bernardo Preso, Ryan Holman

Sonosensitive perfluorocarbon F(8)TAC(18)-PFOB emulsion is under development to enhance heating, increase thermal contrast, and reduce treatment times during focused ultrasound tumor ablation of highly perfused tissue. The emulsion previously showed enhanc ...

FRONTIERS MEDIA SA2023

Volumetric additive manufacturing in a scattering resin

Jorge Andres Madrid Wolff

Volumetric 3D printing is a novel technique that offers promising new perspectives in tissue engineering. In volumetric 3D printing, photosensitive gels or liquids are solidified by projecting light patterns via reverse tomography. Recent results show that ...

EPFL2023

Neuronal migration prevents spatial competition in retinal morphogenesis

Martin Weigert

The concomitant occurrence of tissue growth and organization is a hallmark of organismal development(1-3). This often means that proliferating and differentiating cells are found at the same time in a continuously changing tissue environment. How cells ada ...

NATURE PORTFOLIO2023

Elucidating and modulating regulatory pathways so as to reprogram the immunosuppressive tumor microenvironment for therapeutic benefit

Agnieszka Alicja Chryplewicz

Glioblastoma (GBM) is the most frequent and highly malignant form of brain cancer. New therapeutic regimens are urgently needed as GBM patients present with a minimal response to currently-approved therapies. The goal of this project is to identify new the ...

EPFL2023

Mechanical Characterisation of Porcine Urethra: Non Linear Constitutive Models and Experimental Approach

Yves Perriard, Yoan René Cyrille Civet, Thomas Guillaume Martinez, Stefania Maria Aliki Konstantinidi, Amine Benouhiba, Quentin Philippe Mario De Menech

Low urinary tract dysfunctions and symptoms (LUTS) and Urinary incontinence (UI) in particular is a medical condition that affects millions of people worldwide. One of the most promising long term treatments for this condition is the use of an artificial u ...

IEEE2023

Methods and devices for cultivating coenocytic algae

Christian Ludwig, Enio Daniel Zanchetta, Eya Nicodeme

The present invention concerns methods and devices for cultivating coenocytic algae and for producing cellulose, in particular microfibrillated cellulose (MFC). In one embodiment, the algae are grown on a solid substrate immersed in liquid culture medium. ...

2023

Actuated 3D microgels for single cell mechanobiology

Mahmut Selman Sakar, Ece Özelçi, Berna Ayse Özkale Edelmann, Christina Myra Tringides

We present a new cell culture technology for large-scale mechanobiology studies capable of generating and applying optically controlled uniform compression on single cells in 3D. Mesenchymal stem cells (MSCs) are individually encapsulated inside an optical ...

ROYAL SOC CHEMISTRY2022

Intrinsically High Capacity of Animal Cells From a Symbiotic Cnidarian to Deal With Pro-Oxidative Conditions

Gaëlle Delphine Toullec

The cnidarian-dinoflagellate symbiosis is a mutualistic intracellular association based on the photosynthetic activity of the endosymbiont. This relationship involves significant constraints and requires co-evolution processes, such as an extensive capacit ...

FRONTIERS MEDIA SA2022

In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging

Patrick Descombes, Jérome Feige, Sylviane Métairon, Omid Mashinchian, Filippo De Franceschi, Nicolas Bouche, Christophe Boss

Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specifi ...

2022

Organoids as tools for fundamental discovery and translation-A Keystone Symposia report

Matthias Lütolf

Complex three-dimensional in vitro organ-like models, or organoids, offer a unique biological tool with distinct advantages over two-dimensional cell culture systems, which can be too simplistic, and animal models, which can be too complex and may fail to ...

WILEY2022

An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates

Matthias Lütolf, Sebastian Maerkl, Hao-An Hsiung, Zoe Newell Swank, Stefano Davide Vianello, Julia Tischler

We present a low-cost, do-it-yourself system for complexmammalian cell culture under dynamically changing medium formulations by integrating conventional multi-well tissue culture plates with simple microfluidic control and system automation. We demonstrat ...

2022

Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis

Didier Trono, Laurence Gouzi Abrami, Sandra Eloise Kjeldsen, Evaristo Jose Planet Letschert, Julien Léonard Duc, Laia Simo Riudalbas, Alexandre Coudray, Sagane Dind

The treatment of colorectal cancer (CRC) is an unmet medical need in absence of early diagnosis. Here, upon characterizing cancer-specific transposable element-driven transpochimeric gene transcripts (TcGTs) produced by this tumor in the SYSCOL cohort, we ...

Nature Research2022

Identification of broad anti-coronavirus chemical agents for repurposing against SARS-CoV-2 and variants of concern

Gerardo Turcatti, Fabien Kuttler, Michael Bauer, Liliane Yang

Endemic human coronaviruses (hCoVs) 229E and OC43 cause respiratory disease with recurrent infections, while severe acute respiratory syndrome (SARS)-CoV-2 spreads across the world with impact on health and societies. Here, we report an image-based multicy ...

2022

Dendritic cell cross-dressing and tumor immunity

Michele De Palma, Amaia Martinez Usatorre

In addition to direct and cross-presentation, dendritic cells (DCs) can present tumor antigens (TAs) to T cells via a hitherto poorly understood mechanism called "cross-dressing." DC cross-dressing involves the acquisition of preformed peptide-major histoc ...

WILEY2022

Prediction of Dispersion Rate of Airborne Nanoparticles in a Gas-Liquid Dual-Microchannel Separated by a Porous Membrane: A Numerical Study

Carlotta Guiducci, Zohreh Sheidaei

Recently, there has been increasing attention toward inhaled nanoparticles (NPs) to develop inhalation therapies for diseases associated with the pulmonary system and investigate the toxic effects of hazardous environmental particles on human lung health. ...

MDPI2022

Hallmarks of Cancer: New Dimensions

Douglas Hanahan

The hallmarks of cancer conceptualization is a heuristic tool for distilling the vast complexity of cancer phenotypes and genotypes into a provisional set of underl y-ing principles. As knowledge of cancer mechanisms has progressed, other facets of the dis ...

AMER ASSOC CANCER RESEARCH2022