Publications associées à Transformation (génétique)

Dangerous acquaintances: the interplay between type IV pili and the type VI secretion system during Vibrio cholerae's environmental lifestyle

Cholera, caused by the bacterium Vibrio cholerae, has affected humanity throughout history and still impacts millions of people every year. Apart from being a human pathogen, V. cholerae is a common member of the aquatic environment. Due to this natural re ...

EPFL2024

Biotechnological Frontiers of DNA Nanomaterials Continue to Expand: Bacterial Infection using Virus-Inspired Capsids

Maartje Martina Cornelia Bastings

The elegant geometry of viruses has inspired bio-engineers to synthetically explore the self-assembly of polyhedral capsids employed to protect new cargo or change an enzymatic microenvironment. Recently, Yang and co-workers used DNA nanotechnology to revi ...

Wiley-VCH Verlag GmbH2023

DNA modifications impact natural transformation of Acinetobacter baumannii

Melanie Blokesch, Nicolas Jean Philippe Guex, Christian Iseli, Alexandre Lemopoulos, Nina Vesel

Acinetobacter baumannii is a dangerous nosocomial pathogen, especially due to its ability to rapidly acquire new genetic traits, including antibiotic resistance genes (ARG). In A. baumannii, natural competence for transformation, one of the primary modes o ...

2023

Engineering Agrobacterium tumefaciens Adhesion to Target Cells

Alexandre Louis André Persat, Xavier Jean-Yves Pierrat, Jeremy Pak Hei Wong, Alix Kim Lan Pham

Agrobacterium tumefaciens is a plant pathogen commonly repurposed for genetic modification of crops. Despite its versatility, it remains inefficient at transferring DNA to many hosts, including to animal cells. Like many pathogens, physical contact between ...

AMER CHEMICAL SOC2022

Natural competence of the pathogen Acinetobacter baumannii: an elusive phenomenon

Nina Vesel

Natural competence for transformation is an important driver of horizontal DNA exchange between different organisms. This can result in accumulation of dangerous genetic features, such as antibiotic resistance genes, in a single organism. One example of an ...

EPFL2022

Engineering synthetic adhesins for biophysical investigation and rewiring of host-microbe interactions

Xavier Jean-Yves Pierrat

Host attachment is often a critical step in the onset of pathogenesis. To attach to host cells, bacteria have evolved a range of adhesins that bind to specific receptors. Some of these adhesins have been thoroughly characterized using biochemical technique ...

EPFL2022

Engineering of chitosan-based derivatives for non-viral gene delivery

Laura Camille Louise Nicolle

Gene therapy offers the possibility to treat or even cure diseases originating from genetic defects by introducing a therapeutic gene in target cells or correcting the initial defective gene. Amongst different options, non-viral vectors rely on the deliver ...

EPFL2022

Measurements of AC Loss Evolution in ITER TF Conductors

Pierluigi Bruzzone, Kamil Sedlák

AC loss in the Nb3Sn cable-in-conduit conductors (CICC) usually decreases after the conductor undergoes electromagnetic cycling. This can be attributed to the increase of inter-strand resistance due to the detachment of the strand-bonding during cyclic loa ...

IEEE Institute of Electrical and Electronics Engineers2022

An optimized high throughput platform, from genetic transformation to fermentation, to screen for secreted laccases in Aspergillus niger

Laure Marie Colette Leynaud-Kieffer Curran

Many challenges are faced in the conversion of biomass into advanced biofuels, one of which is finding the correct organism for the job. The filamentous fungus Aspergillus niger has been chosen as a biocatalyst for cellulose, hemicellulose, and lignin degr ...

EPFL2021

Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae

Melanie Blokesch, David William Adams, Milena Jadwiga Jaskolska

Horizontal gene transfer can trigger rapid shifts in bacterial evolution. Driven by a variety of mobile genetic elements—in particular bacteriophages and plasmids—the ability to share genes within and across species underpins the exceptional adaptability o ...

2022

Pilus production in Acinetobacter baumannii is growth phase dependent and essential for natural transformation

Melanie Blokesch, Nina Vesel

Acinetobacter baumannii is a severe threat to human health as a frequently multidrug-resistant hospital-acquired pathogen. Part of the danger from this bacterium comes from its genome plasticity and ability to evolve quickly by taking up and recombining ex ...

American Society for Microbiology2021

Clofarabine Commandeers the RNR-alpha-ZRANB3 Nuclear Signaling Axis

Yimon Aye, Yi Zhao

Ribonucleotide reductase (RNR) is an essential enzyme in DNA biogenesis and a target of several chemotherapeutics. Here, we investigate how antileukemic drugs (e.g., clofarabine [CIF]) that target one of the two subunits of RNR, RNR-alpha, affect noncanoni ...

CELL PRESS2020

Synthetic Studies toward Amanita Phalloides Toxins

Philipp Georg Werner Seeberger

Amatoxins are ribosomally synthesized and post-translationally modified bicyclic octapeptides biosynthesized by the deadly basidiomycete fungus Amanita phalloides. Amongst this group, alpha-amanitin is the most widely known toxin and is currently under inv ...

EPFL2020

Comparison of chitin‐induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains

Melanie Blokesch, Sandrine Stutzmann

The human pathogen Vibrio cholerae serves as a model organism for many important processes ranging from pathogenesis to natural transformation, which has been extensively studied in this bacterium. Previous work has deciphered important regulatory circuits ...

2020

Measurement and prediction of the transformation strain that controls ductility and toughness in advanced steels

William Curtin, Helena Van Swygenhoven, Francesco Maresca, Efthymios Polatidis

New-generation multi-phase martensitic steels derive their high strength from the body-centered cubic (BCC) phase and high toughness from transformation of the metastable face-centered cubic (FCC) austenite that transforms into martensite upon loading. In ...

PERGAMON-ELSEVIER SCIENCE LTD2020

Growing Ungrowable Bacteria: Overview and Perspectives on Insect Symbiont Culturability

Bruno Lemaitre, Florent François Masson

Insects are often involved in endosymbiosis, that is, the housing of symbiotic microbes within their tissues or within their cells. Endosymbionts are a major driving force in insects' evolution, because they dramatically affect their host physiology and al ...

AMER SOC MICROBIOLOGY2020

Establishing a Ternary System for Optical Monitoring of DNA-Protein Interactions with Single-Walled Carbon Nanotubes

Shang-Jung Wu

DNA-protein interactions lie at the crux of life's essential processes. As such, various technologies have been developed to characterize these interactions. The distinct advantages of these technologies can be leveraged to study different facets of these ...

EPFL2020

Travel Time and Source Variation Explain the Molecular Transformation of Dissolved Organic Matter in an Alpine Stream Network

Tom Ian Battin, Hannes Markus Peter, Amber Joy Ulseth

Streams and rivers are important components of the carbon cycle as they transport and transform dissolved organic matter (DOM). Using high resolution Fourier transform ion cyclotron resonance mass spectrometry, we studied the spatial distribution of DOM at ...

2020

DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction

Melanie Blokesch, Sandrine Stutzmann, Candice Stoudmann, David William Adams

How bacteria colonize surfaces and how they distinguish the individuals around them are fundamental biological questions. Type IV pili are a widespread and multipurpose class of cell surface polymers. Here we directly visualize the DNA-uptake pilus of Vibr ...

2019

Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae

Melanie Blokesch, Nicolas Jean Philippe Guex, Sandrine Stutzmann, Christian Iseli, Candice Stoudmann, Noémie Matthey

Natural competence for transformation is a primary mode of horizontal gene transfer. Competent bacteria are able to absorb free DNA from their surroundings and exchange this DNA against pieces of their own genome when sufficiently homologous. However, the ...

2019