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Publications associées (32)

3D nanometrology of transparent objects by phase calibration of a basic bright-field microscope for multiple illumination apertures

Martinus Gijs, Daniel Migliozzi, Bingying Zhao

Optical retrieval of the structure of transparent objects at the nanoscale requires adapted techniques capable of probing their interaction with light. Here, we considered a method based on calibration of the defocusing with partially coherent illumination ...

2020

Active illumination and computational Methods for Temporal and Spectral Super-Resolution Microscopy

Christian Jaques

Light microscopy is a tool of paramount importance for biologists and has been constantly improved for the past four centuries. Despite many recent developments, microscopy techniques still require improvement, especially to reach better temporal and spect ...

EPFL2020

Near-field phase profile and far-field contrast modulation for micro-lens arrays at the refraction limit

Toralf Scharf, Maryam Yousefi, Daniel Necesal

We investigate the far-field pattern generation for a micro-lens array (MLA) illuminated under different conditions. Plane wave and Gaussian beam illumination are considered for an MLA with a small diameter of 27 microns and 30 microns period. At these dim ...

2020

Graph-based regularization of inverse problems in imaging

Mattia Rossi

As of today, the extension of the human visual capabilities to machines remains both a cornerstone and an open challenge in the attempt to develop intelligent systems. On the one hand, the development of more and more sophisticated imaging devices, capable ...

EPFL2020

Released Diclofenac Sodium Salt From Chitosan Embedding In A Hydrogel Matrix. A Theoretical And Experimental Study

Maria-Alexandra Paun

Two formulations embedding diclofenac sodium salt into a chitosan hydrogel matrix with different crosslinking degree were prepared by in situ hydrogelation. The formulations were investigated by polarized light microscopy and scanning electron microscopy, ...

UNIV POLITEHNICA BUCHAREST, SCI BULL2020

5-Fluorouracil Release from Chitosan-Based Matrix. Experimental and Theoretical Aspects

Maria-Alexandra Paun

A series of four drug release formulations based on 5-fluorouracil encapsulated into a chitosan-based matrix were prepared by in situ hydrogelation with 3,7-dimethyl-2,6-octadienal. The formulations were investigated from structural and morphological aspec ...

2020

W2S: Microscopy Data with Joint Denoising and Super-Resolution for Widefield to SIM Mapping

Sabine Süsstrunk, Daniel Sage, Thierry Laroche, Arne Seitz, Majed El Helou, Ruofan Zhou

In fluorescence microscopy live-cell imaging, there is a critical trade-off between the signal-to-noise ratio and spatial resolution on one side, and the integrity of the biological sample on the other side. To obtain clean high-resolution (HR) images, one ...

2020

Experimental And Theoretical Insight Into Formulations Based On Poly(Vinyl Alcohol Boric Acid) And Diclofenac Sodium Salt

Maria-Alexandra Paun

A series of three formulations were prepared in view of experimental and theoretical investigation of their drug release potential. The morphology of the film formulations, in terms of distribution of drug into the polymeric matrix and the nature of the dr ...

UNIV POLITEHNICA BUCHAREST, SCI BULL2020

Phase resolved characterization of nanoscale features in optical microstructures

Michail Symeonidis

In this study, we apply interferometric microscopy to study the phase, alongside the intensity, of the light field transmitted through a wide variety of samples. Additionally, we conduct those interferometric measurements at different wavelengths within th ...

EPFL2019

Transmission Microsphere-Assisted Dark-Field Microscopy

Giorgio Quaranta, Stéphane Perrin, Hongyu Li

Microsphere-assisted microscopy allows the limit of the diffraction of light to overcome while being non-invasive, full-field, label-free, and easy-to-implement. However, the observation of translucent samples remains difficult using a classical bright-fie ...

WILEY-V C H VERLAG GMBH2019

Hard Phase Crystallization Directs the Phase Segregation of Hydrogen-Bonded Supramolecular Polymers

Emad Oveisi

A growing body of work shows that the phase behavior of supramolecular polymers assembled from telechelic building blocks featuring binding motifs at the two termini is quite similar to that of conventional block copolymers. However, it remains unclear how ...

American Chemical Society2019

Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos

Andrew Charles Oates, Rachna Narayanan

In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non- ...

2019

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