Publications associées à Caenorhabditis briggsae

Drug Screening in C. elegans to identify new pharmacological mitochondrial stress inducers

Mitochondria are essential organelles participating in numerous cellular functions, including energy harvesting, regulation of homeostasis and apoptosis. Changes in mitochondrial number, morphology, and function not only impact cellular metabolism but also ...

EPFL2024

Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage Caenorhabditis elegans embryos

Pierre Gönczy, Fernando Romero Balestra

Whereas both sperm and egg contribute nuclear genetic material to the zygote in metazoan organisms, the inheritance of other cellular constituents is unequal between the 2 gametes. Thus, 2 copies of the centriole are contributed solely by the sperm to the ...

GENETICS SOCIETY AMERICA2023

High-resolution imaging and analysis of the intestinal bacterial load of Caenorhabditis elegans during early adulthood

Martinus Gijs, Farzad Rezaeianaran

We study the presence within the worm Caenorhabditis elegans (C. elegans) of a fluorescent strain of the worm's bacterial food (Escherichia coli (E. coli) OP50) during early adulthood. Use of a microfluidic chip based on a thin glass coverslip substrate al ...

ROYAL SOC CHEMISTRY2023

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

Farzad Rezaeianaran

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

EPFL2023

Centriole elimination during Caenorhabditis elegans oogenesis initiates with loss of the central tube protein SAS-1

Pierre Gönczy, Coralie Busso, Alexander Woglar, Mary-Claude Croisier-Coeytaux, Tamara Mikeladze-Dvali, Keshav Jha, Marie Juliette Ségolène Pierron

In most metazoans, centrioles are lost during oogenesis, ensuring that the zygote is endowed with the correct number of two centrioles, which are paternally contributed. How centriole architecture is dismantled during oogenesis is not understood. Here, we ...

Wiley2023

Deformation-aware Unpaired Image Translation for Pose Estimation on Laboratory Animals

Pascal Fua, Pavan P Ramdya, Helge Jochen Rhodin, Semih Günel, Siyuan Li, Mirela Ostrek

Our goal is to capture the pose of neuroscience model organisms, without using any manual supervision, to be able to study how neural circuits orchestrate behaviour. Human pose estimation attains remarkable accuracy when trained on real or simulated datase ...

2020

Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform

Martinus Gijs, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hüseyin Baris Atakan, Tunç Alkanat

The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the sev ...

2020

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Rongrong Xiang, Elena Katsyuba, Hüseyin Baris Atakan

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

Automated high-content phenotyping from the first larval stage till the onset of adulthood of the nematode Caenorhabditis elegans

Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan

The nematode Caenorhabditis elegans is increasingly used as a model for human biology. However, in vivo culturing platforms for C. elegans allowing high-content phenotyping during their life cycle in an automated fashion are lacking so far. Here, a multipl ...

ROYAL SOC CHEMISTRY2019

Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment

Pierre Gönczy, Alexandra Bezler, Frédéric Schütz, Laurent Keller, Raffaella Conconi

RNA interference (RNAi) related pathways are essential for germline development and fertility in metazoa and can contribute to inter- and trans-generational inheritance. In the nematode Caenorhabditis elegans, environmental double-stranded RNA provided by ...

2019

Aurora A depletion reveals centrosome-independent polarization mechanism in Caenorhabditis elegans

Pierre Gönczy, Coralie Busso, Kerstin Klinkert Fabbri, Lukas von Tobel, Christian Gentili, Marie Juliette Ségolène Pierron, Sarah Herrman

How living systems break symmetry in an organized manner is a fundamental question in biology. In wild-type Caenorhabditis elegans zygotes, symmetry breaking during anterior-posterior axis specification is guided by centrosomes, resulting in anterior-direc ...

2019

Microfluidic and micromechanical in vivo studies of Caenorhabditis elegans

Roger Philippe Krenger

Caenorhabditis elegans is one of the most attractive model organisms in biomedical research for understanding human diseases and for drug testing at a whole-organism level, since many biological pathways and genes have been conserved between itself and hum ...

EPFL2019

8-Channel Single Embryo Pipette For Accurate C-Elegans Bioassays

Martinus Gijs, Thomas Lehnert, Li Dong, Jingwei Zhang

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or bioch ...

IEEE2018

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Li Dong

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

EPFL2018

Microfluidic high-content phenotypic screening on a Caenorhabditis elegans model

Maria Cristina Letizia

Caenorhabditis elegans is a performant model system for studying human biological processes and diseases and for pre-clinical phenotyping screenings of compounds. Microfluidics has been instrumental in enabling C. elegans-based drug assays, facilitating th ...

EPFL2018

A microfluidic platform for automated phenotyping during full lifespan of Caenorhabditis elegans at high-throughput

Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan

We present a multiplexed and automated microfluidic platform for phenotyping of C. elegans during their full lifespan and at high-throughput. We used the fluorescent hsp-6::gfp strain of worms and performed experiments to observe the effect of four differe ...

2017

Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

Johan Auwerx, Theo Lasser, Laurent Mouchiroud, Paul James Marchand, Arno Pino Bouwens, Vincenzo Sorrentino, Séverine Coquoz, Daniel Pawel Szlag

Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the ne ...

Public Library of Science2017

Enhancing mitochondrial proteostasis reduces amyloid-beta proteotoxicity

Johan Auwerx, Davide D'Amico, Adrian Schmid, Mario Romani, Norman Lionel Moullan, Vincenzo Sorrentino, Hongbo Zhang, Francesca Potenza, Solene Rietsch

Alzheimer's disease is a common and devastating disease characterized by aggregation of the amyloid-beta peptide. However, we know relatively little about the underlying molecular mechanisms or how to treat patients with Alzheimer's disease. Here we provid ...

2017

Versatile size-dependent sorting of C-elegans nematodes and embryos using a tunable microfluidic filter structure

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

The roundworm Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Its life cycle consists of an embryo stage, four larval stages that can be clearly distinguis ...

Royal Soc Chemistry2016

On-chip microfluidic biocommunication assay for studying male-induced demise in C. elegans hermaphrodites

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

Like other animals, C. elegans nematodes have the ability to socially interact and to communicate through exchange and sensing of small soluble signaling compounds that help them cope with complex environmental conditions. For the time being, worm biocommu ...

Royal Soc Chemistry2016