Cette séance de cours couvre les techniques d'étiquetage des protéines en utilisant des pièces d'auto-étiquetage comme FIASH et SNAP, en discutant des facteurs importants pour l'étiquetage des cellules vivantes, la spécificité, la vitesse, la taille des étiquettes et la disponibilité du substrat. Il compare également deux systèmes: FIASH vs SNAP.
Matteo Dal Peraro graduated in Physics at the University of Padua in 2000. He obtained his Ph.D. in Biophysics at the International School for Advanced Studies (SISSA, Trieste) in 2004. He then received postdoctoral training at the University of Pennsylvania (Philadelphia) under the guidance of Prof. M. L. Klein. He was nominated Tenure Track Assistant Professor at the EPFL School of Life Sciences in late 2007.His research at the Laboratory for Biomolecular Modeling (LBM), within the Interfaculty Institute of Bioengineering (IBI), focuses on the multiscale modeling of large macromolecular systems.
Throughout my PhD and postdoctoral studies I was trained in world-renowned laboratories and institutions in the United States of America (University of Washington and The Scripps Research Institute). Very early in my scientific career I found out my fascination about protein structure and function. My PhD studies evolved in the direction of immunogen design and vaccine engineering which sparked my interest in the many needs and opportunities in vaccinology and translational research. My efforts resulted in an enlightening piece of work where for the first time, computationally designed immunogens elicited potent neutralizing antibodies. During my postdoctoral studies I joined a chemical biology laboratory at the Scripps Research Institute. In this stage I developed novel chemoproteomics methods for the identification of protein-small molecule interaction sites in complex proteomes. In March 2015, I joined the École Polytechnique Fédérale de Lausanne (EPFL) – Switzerland as a tenure track assistant professor. The focus of my research group is to develop computational tools for protein design with particular emphasis in applying these strategies to immunoengineering (e.g. vaccine and cancer immunotherapy). The activities in my laboratory focus on computational design methods development and experimental characterization of the designed proteins. Our laboratory has been awarded with 2 prestigious research grants from the European Research Council. Lastly, I have been awarded the prize for best teacher of Life sciences in 2019.
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Biochemistry is a key discipline for the Life Sciences. Biological Chemistry I and II are two tightly interconnected courses that aim to describe and understand in molecular terms the processes that m
Couvre les principes de la microscopie électronique à balayage, y compris les signaux SEM, les détecteurs et le spectre d'énergie des électrons, ainsi que l'efficacité de la génération de rayons X.
Explore les caractéristiques de la turbulence, les méthodes de simulation et les défis de modélisation, fournissant des lignes directrices pour le choix et la validation des modèles de turbulence.
Explore l'évolution et la fonction des machines à réparer les protéines, en mettant l'accent sur le rôle des machines à déployer alimentées à l'ATP dans la prévention de l'agrégation des protéines et la promotion d'un pliage approprié.
Explore les réseaux d'interaction protéines-protéines, l'importance de la liaison, les approches expérimentales, l'identification des cibles médicamenteuses et la construction de réseaux.
