Philippe Pierre Girard

À propos
Confidentialité
Mentions légales

Graph Chatbot

Publications associées (6)

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Madiha Derouazi

Microinjection is a gene transfer technique enabling partial control of plasmid delivery into the nucleus or cytoplasm of cultured animal cells. Here this method was used to establish various recombinant mammalian cell lines. The injection volume was estim ...

Kluwer Academic Publishers, Springer2006

Orbital shaker technology for the cultivation of mammalian cells in suspension

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Natalie Muller, David Hacker

For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior ...

2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Philippe Pierre Girard, Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Philippe Pierre Girard, Florian Maria Wurm, Lucia Baldi Unser, David Hacker

Springer2003

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Jason Wright, Lucia Baldi Unser, Frédéric Grosjean, Natalie Muller, David Hacker

Springer2002

Rapid protein expression in mammalian cells : large-scale transient transfection

Philippe Pierre Girard

EPFL2002

Page 1 sur 1