Publications de Andrzej Kulik | EPFL Graph Search

Infrared nanospectroscopic mapping of a single metaphase chromosome

Andrzej Kulik, Giovanni Dietler, Francesco Simone Ruggeri, Ewelina Wioletta Lipiec

The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy ...

Oxford University Press (OUP)2019

Nanomechanics of multidomain neuronal cell adhesion protein contactin revealed by single molecule AFM and SMD (vol 7, 2017)

Andrzej Kulik, Giovanni Dietler, Karolina Anna Mikulska Ruminska

Nature Research2018

Nanoscale Investigation into the Cellular Response of Glioblastoma Cells Exposed to Protons

Andrzej Kulik, Giovanni Dietler, Ewelina Wioletta Lipiec

2018

Nanomechanics of multidomain neuronal cell adhesion protein contactin revealed by single molecule AFM and SMD

Andrzej Kulik, Giovanni Dietler, Karolina Anna Mikulska Ruminska

Contactin-4 (CNTN4) is a complex cell adhesion molecule (CAM) localized at neuronal membranes, playing a key role in maintaining the mechanical integrity and signaling properties of the synapse. CNTN4 consists of six immunoglobulin C2 type (IgC2) domains a ...

Springer Nature2017

MechStiff: A New Tool for Evaluating Stress-Induced Dynamics and Application to Cell Adhesion Proteins

Andrzej Kulik, Giovanni Dietler, Karolina Anna Mikulska Ruminska

Cell Press2017

Can Dissipative Properties of Single Molecules Be Extracted from a Force Spectroscopy Experiment?

Andrzej Kulik, Giovanni Dietler, Serguei Sekatski, Dmitry Klinov, Fabrizio Benedetti

We performed dynamic force spectroscopy of single dextran and titin 127 molecules using small-amplitude and low-frequency (40-240 Hz) dithering of an atomic force microscope tip excited by a sine wave voltage fed onto the tip-carrying piezo. We show that f ...

Elsevier2016

Nanoscale resolution in infrared imaging of protein-containing lipid membranes

Andrzej Kulik, Giovanni Dietler

The precise imaging of biomolecular entities contributes to an understanding of the relationship between their structure and function. However, the resolution of conventional infrared microscopic imaging is diffraction limited and does not exceed a few mic ...

Royal Society of Chemistry2015

Probing fibronectin-antibody interactions using AFM force spectroscopy and lateral force microscopy

Andrzej Kulik, Kyumin Lee

The first experiment showing the effects of specific interaction forces using lateral force microscopy (LFM) was demonstrated for lectin-carbohydrate interactions some years ago. Such measurements are possible under the assumption that specific forces stro ...

Beilstein-Institut2015

Nanoscale spatially resolved infrared spectra from single microdroplets

Andrzej Kulik, Giovanni Dietler, Francesco Simone Ruggeri

Droplet microfluidics has emerged as a powerful platform allowing a large number of individual reactions to be carried out in spatially distinct microcompartments. Due to their small size, however, the spectroscopic characterisation of species encapsulated ...

Royal Society of Chemistry2014

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Harald Brune, Andrzej Kulik, Nicolas Mermod, Giovanni Dietler, Guillaume Witz, Stefania Puttini

Aim: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. Materials & methods: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patient ...

Future Medicine2014

Innovative transparent non-scattering TiO2 bactericide thin films inducing increased E. coli cell wall fluidity

Juan Kiwi, Lioubov Kiwi, Andrzej Kulik, César Pulgarin, Rosendo Sanjines, Sami Rtimi, Mariusz Andrzejczuk

This study presents the first account for transparent, non-scattering TiO2 films on radio-frequency (RF) plasma and ultraviolet light in the C-spectral region (UVC) pretreated polyethylene (PE). These pretreatments allow the PE to bind higher amounts of Ti ...

Elsevier Science Sa2014

Nanoscale infrared imaging of lipoprotein multilayers

Andrzej Kulik

Springer2013

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Andrzej Kulik, Nicolas Mermod, Damien Saugy, Stefania Puttini, Déborah Ley

Background: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. Results: In this study, we devised vectors allowing a tight regulatio ...

BioMed Central2013

A single-molecule stretching method for lateral and normal AFM lever calibration

Andrzej Kulik, Giovanni Dietler, Fabrizio Benedetti

A novel method for quantitative lateral force measurement (LFM) calibration has been developed. Using a single-molecule spectroscopy approach it is possible to calibrate the AFM levers for both lateral and normal spring constants with a single image scan. ...

Institute of Physics2013

Photocatalytic and phototoxic properties of TiO2-based nanofilaments: ESR and AFM assays

László Forró, Andrzej Kulik, Andrzej Sienkiewicz, Arnaud Magrez, Katarzyna Pierzchala

There is uncertainty in understanding of the relationship between physico-chemical parameters of nanosized titanium dioxide (nano-TiO2) and its toxicity when brought into contact with living cells. This study provides a multidisciplinary experimental insig ...

Informa Healthcare2012

E. coli Inactivation by High-Power Impulse Magnetron Sputtered (HIPIMS) Cu Surfaces

Juan Kiwi, Andrzej Kulik, César Pulgarin, Oualid Baghriche

This study reports HIPIMS-sputtered samples of Cu-particulate films with currents at 6 and 60 amps leading to E. coil inactivation. The Cu coverage and nanoparticle structure of the fibers is reported by TEM. Evidence is presented of redox processes in the ...

2011

Characterization of N-cadherin unbinding properties in non-malignant (HCV29) and malignant (T24) bladder cells

Andrzej Kulik

The expression of N-cadherin, characteristic of various cancers, very often leads to changes in the cells' adhesive properties. Thus, we sought to find out if N-cadherin expressed in various, but cancer-related cells, differs in its functional properties t ...

Wiley-Blackwell2011

Model-Based Estimation of Three-Dimensional Stiffness Parameters in Photonic-Force Microscopy

Andrzej Kulik, Philippe Thévenaz, Michaël Unser, Elena Bertseva, Amardeep Singh

We propose a system to characterize the 3-D diffusion properties of the probing bead trapped by a photonic-force microscope. We follow a model-based approach, where the model of the dynamics of the bead is given by the Langevin equation. Our procedure comb ...

Institute of Electrical and Electronics Engineers2010

AFM Force Spectroscopy and Steered Molecular Dynamics Simulation of Protein Contactin 4

Andrzej Kulik, Karolina Anna Mikulska Ruminska

We use a single molecule atomic force spectroscopy combined with the steered molecular dynamics simulation to determine a mechanical behavior of neural cell adhesion protein contactin during its unfolding. Force curves typical for modular proteins were obs ...

Polish Academy of Sciences Warsaw2009

Intracellular nanomanipulation by a photonic-force microscope with real-time acquisition of a 3D stiffness matrix

László Forró, Gérard Gremaud, Andrzej Kulik, Philippe Thévenaz, Michaël Unser, Giovanni Dietler, Stefania Puttini, Sylvia Jeney, Elena Bertseva, Amardeep Singh

A traditional photonic-force microscope (PFM) results in huge sets of data, which requires tedious numerical analysis. In this paper, we propose instead an analog signal processor to attain real-time capabilities while retaining the richness of the traditi ...

Institute of Physics2009