Publications de Jean-Jacques Meister

Intercellular Ultrafast Calcium Wave Velocity and Propagation of Spontaneous Electrical Activity in A7r5 Cells at Physiological Temperature

Jean-Jacques Meister, Jairo Camilo Quijano Perez

Cell Press2018

Minimal model for spontaneous cell polarization and edge activity in oscillating, rotating and migrating cells

Jean-Jacques Meister, Alexander Verkhovskiy, Mark Etienne Ambühl, Chiara Gabella, Franck Claude Raynaud, Alicia Carine Bornert

How cells break symmetry and organize activity at their edges to move directionally is a fundamental question in cell biology. Physical models of cell motility commonly incorporate gradients of regulatory proteins and/or feedback from the motion itself to ...

Nature Publishing Group2016

Intercellular ultrafast Ca2+ wave in vascular smooth muscle cells: numerical and experimental study

Jean-Jacques Meister, Niccolo Piacentini, Jairo Camilo Quijano Perez, Franck Claude Raynaud

Vascular smooth muscle cells exhibit intercellular Ca2+ waves in response to local mechanical or KCl stimulation. Recently, a new type of intercellular Ca2+ wave was observed in vitro in a linear arrangement of smooth muscle cells. The intercellular wave w ...

Nature Research2016

Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Benoît Vianay, Céline Jeanne Labouesse

Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through ...

Nature Research2016

Cell Shape Dynamics Reveal Balance of Elasticity and Contractility in Peripheral Arcs

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Benoît Vianay, Céline Jeanne Labouesse

The mechanical interaction between adherent cells and their substrate relies on the formation of adhesion sites and on the stabilization of contractile acto-myosin bundles, or stress fibers. The shape of the cell and the orientation of these fibers can be ...

Cell Press2015

Evaluating Tension in Actomyosin Bundles at the Cell Periphery

Jean-Jacques Meister, Niccolo Piacentini, Benoît Vianay, Céline Jeanne Labouesse

Cell Press2014

Ultra-soft cantilevers and 3-D micro-patterned substrates for contractile bundle tension measurement in living cells

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Niccolo Piacentini, Benoît Vianay

Actin-myosin microfilament bundles or stress-fibers are the principal tension-generating structures in the cell. Their mechanical properties are critical for cell shape, motion, and interaction with other cells and extracellular matrix, but were so far dif ...

Royal Society of Chemistry2014

Contact Angle at the Leading Edge Controls Cell Protrusion Rate

László Forró, Jean-Jacques Meister, Alexander Verkhovskiy, Sylvia Jeney, Chiara Gabella, Elena Bertseva, Niccolo Piacentini, Alicia Carine Bornert

Plasma membrane tension and the pressure generated by actin polymerization are two antagonistic forces believed to define the protrusion rate at the leading edge of migrating cells [1-5]. Quantitatively, resistance to actin protrusion is a product of membr ...

Elsevier2014

Ultrafast Calcium Wave in Cultured Vascular Smooth Muscle Cells

Jean-Jacques Meister, Jairo Camilo Quijano Perez

Cell Press2014

Actin Bundle Stabilization During Cell Spreading on Micropatterned Substrates

Jean-Jacques Meister, Josiane Angèle Charlotte Smith-Clerc, Benoît Vianay

Cell Press2014

The Nano-Scale Mechanical Properties of the Extracellular Matrix Regulate Dermal Fibroblast Function

Jean-Jacques Meister, Boris Hinz, Josiane Angèle Charlotte Smith-Clerc, Lara Buscemi Estefanell

Changes in the mechanical properties of dermis occur during skin aging or tissue remodeling and affect the activity of resident fibroblasts. With the aim to establish elastic culture substrates that reproduce the variable softness of dermis, we determined ...

Nature Publishing Group2014

Ultrafast Ca2+ wave in cultured vascular smooth muscle cells aligned on a micropatterned surface

Jean-Jacques Meister, Jean-Louis Bény, Jairo Camilo Quijano Perez, Benoît Vianay

Communication between vascular smooth muscle cells (SMCs) allows control of their contraction and so regulation of blood flow. The contractile state of SMCs is regulated by cytosolic Ca2+ concentration (Ca2+) which propagates as Ca2+ waves over a sign ...

Elsevier2013

An actin length threshold regulates adhesion maturation at the lamellipodium/lamellum interface

Jean-Jacques Meister, Benoît Vianay, Céline Jeanne Labouesse

The mechanical coupling between adherent cells and their substrates is a major driver of downstream behavior. This coupling relies on the formation of adhesion sites and actin bundles. How cells generate these elements remains only partly understood. A pot ...

Royal Soc Chemistry2013

Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by Connexin43

Jean-Jacques Meister, Jean-Louis Bény, Nadia Halidi

Intercellular Ca2+ wave propagation between vascular smooth muscle cells (SMCs) is associated with the propagation of contraction along the vessel. Here, we characterize the involvement of gap junctions (GJs) in Ca2+ wave propagation between SMCs at the ce ...

2012

High-resolution cell outline segmentation and tracking from phase-contrast microscopy images

Jean-Jacques Meister, Alexander Verkhovskiy, Mark Etienne Ambühl, Charles Brepsant

Accurate extraction of cell outlines from microscopy images is essential for analysing the dynamics of migrating cells. Phase-contrast microscopy is one of the most common and convenient imaging modalities for observing cell motility because it does not re ...

2012

Identification and functional response of interstitial Cajal-like cells from rat mesenteric artery

Jean-Jacques Meister, Jean-Louis Bény, Aurélie Formey de Saint Louvent, Lara Buscemi Estefanell

Cells with irregular shapes, numerous long thin filaments, and morphological similarities to the gastrointestinal interstitial cells of Cajal (ICCs) have been observed in the wall of some blood vessels. These ICC-like cells (ICC-LCs) do not correspond to t ...

Springer-Verlag2011

Propagation of fast and slow intercellular Ca2+ waves in primary cultured arterial smooth muscle cells

Jean-Jacques Meister, Jean-Louis Bény, Nadia Halidi

Smooth muscle contraction is regulated by changes in cytosolic Ca2+ concentration (Ca2+). In response to stimulation, Ca2+ increase in a single cell can propagate to neighbouring cells through gap junctions, as intercellular Ca2+ waves. To investigate ...

2011

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

The Single-Molecule Mechanics of the Latent TGF-beta 1 Complex

Jean-Jacques Meister, Boris Hinz, Josiane Angèle Charlotte Smith-Clerc, Aurélie Formey de Saint Louvent, Lara Buscemi Estefanell, David Ramonet Jimenez

Background: TGF-beta 1 controls many pathophysiological processes including tissue homeostasis, fibrosis, and cancer progression. Together with its latency-associated peptide (LAP), TGF-beta 1 binds to the latent TGF-beta 1-binding protein-1 (LTBP-1), whic ...

Elsevier2011

Interplay between cytoskeletal forces, membrane tension, and hydrostatic pressure in rapidly migrating cells

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Elena Bertseva

Amer Soc Cell Biology2011