Publications de Nicolas Mermod

Overexpression of transcription factor Foxa1 and target genes remediate therapeutic protein production bottlenecks in Chinese hamster ovary cells

Despite extensive research conducted to increase protein production from Chinese hamster ovary (CHO) cells, cellular bottlenecks often remain, hindering high yields. In this study, a transcriptomic analysis led to the identification of 32 genes that are co ...

WILEY2020

miR363-3p mediates maintenance of breast cancer stem cells (BCSCs) and predicts resistance to neoadjuvant chemotherapy and disease recurrence

Nicolas Mermod, Jean-Yves Meuwly, Cathrin Brisken, Valentina Scabia, Valérian Charles Robert Dormoy, Maryse Fiche, Ioannis Xenarios

AMER ASSOC CANCER RESEARCH2020

Transient vitamin B5 starving improves mammalian cell homeostasis and protein production

Nicolas Mermod

Maintaining a metabolic steady state is essential for an organism's fitness and survival when confronted with environmental stress, and metabolic imbalance can be reversed by exposing the organism to fasting. Here, we attempted to apply this physiological ...

ACADEMIC PRESS INC ELSEVIER SCIENCE2020

Autologous Cell Therapy Approach for Duchenne Muscular Dystrophy using PiggyBac Transposons and Mesoangioblasts

Nicolas Mermod

Duchenne muscular dystrophy (DMD) is a lethal muscle-wasting disease currently without cure. We investigated the use of the PiggyBac transposon for full-length dystrophin expression in murine mesoangioblast (MABs) progenitor cells. DMD murine MABs were tra ...

CELL PRESS2018

MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering

Christian Iseli, Nicolas Mermod, Thomas Junier, Samuel Neuenschwander, Ioannis Xenarios

Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous r ...

2017

Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest

Nicolas Mermod, Amar Rida, Etienne Lançon, Niamh Harraghy

We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles an ...

Wiley2017

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Harald Brune, Andrzej Kulik, Nicolas Mermod, Giovanni Dietler, Guillaume Witz, Stefania Puttini

Aim: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. Materials & methods: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patient ...

Future Medicine2014

MAR elements and transposons for improved transgene integration and expression

Nicolas Mermod, Déborah Ley

Elsevier2014

Nuclear Factor I genomic binding associates with chromatin boundaries

Nicolas Mermod, Philipp Bucher, Giovanni Dietler, Jan Kerschgens, Armelle Gaussin, Giovanna Ambrosini, Petar Pjanic

Background: The Nuclear Factor I (NFI) family of DNA binding proteins (also called CCAAT box transcription factors or CTF) is involved in both DNA replication and gene expression regulation. Using chromatin immuno-precipitation and high throughput sequenci ...

BioMed Central2013

MAR Elements and Transposons for Improved Transgene Integration and Expression

Nicolas Mermod, Niamh Harraghy, Déborah Ley

Reliable and long-term expression of transgenes remain significant challenges for gene therapy and biotechnology applications, especially when antibiotic selection procedures are not applicable. In this context, transposons represent attractive gene transf ...

2013

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Andrzej Kulik, Nicolas Mermod, Damien Saugy, Stefania Puttini, Déborah Ley

Background: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. Results: In this study, we devised vectors allowing a tight regulatio ...

BioMed Central2013

Protein-Binding Microarray Analysis of Tumor Suppressor AP2 alpha Target Gene Specificity

Horst Vogel, Nicolas Mermod, Luigino Grasso, Tanja Egener-Kuhn, Jan Kerschgens, Hans-Anton Lehr

Cheap and massively parallel methods to assess the DNA-binding specificity of transcription factors are actively sought, given their prominent regulatory role in cellular processes and diseases. Here we evaluated the use of protein-binding microarrays (PBM ...

Public Library of Science2011

Nuclear factor I revealed as family of promoter binding transcription activators

Nicolas Mermod, Philipp Bucher, Armelle Gaussin, Giovanna Ambrosini, Petar Pjanic

Background: Multiplex experimental assays coupled to computational predictions are being increasingly employed for the simultaneous analysis of many specimens at the genome scale, which quickly generates very large amounts of data. However, inferring valua ...

BioMed Central2011

Gene-mediated Restoration of Normal Myofiber Elasticity in Dystrophic Muscles

Andrzej Kulik, Nicolas Mermod, Damien Saugy, Stefania Puttini

Dystrophin mediates a physical link between the cytoskeleton of muscle fibers and the extracellular matrix, and its absence leads to muscle degeneration and dystrophy. In this article, we show that the lack of dystrophin affects the elasticity of individua ...

2009

Protein-binding microarrays: probing disease markers at the interface of proteomics and genomics

Nicolas Mermod, Tanja Egener-Kuhn, Jan Kerschgens

DNA-binding proteins mediate a variety of crucial molecular functions, such as transcriptional regulation and chromosome maintenance, replication and repair, which in turn control cell division and differentiation. The roles of these proteins in disease ar ...

2009

Mutations in NR2E3 Can Cause Dominant or Recessive Retinal Degenerations in the Same Family

Nicolas Mermod, Pascal Escher

NR2E3, a photoreceptor, specific nuclear receptor (PNR), represses cone-specific genes and activates several rod-specific genes. In humans, mutations in NR2E3 have been associated with the recessively-inherited enhanced short-wavelength sensitive Scone syn ...

2009